Digital Commons Network^™

Cofactor Fingerprinting With Std Nmr To Characterize Proteins Of Unknown Function: Identification Of A Rare Ccmp Cofactor Preference, Huili Yao, Daniel Sem

Chemistry Faculty Research and Publications

Proteomics efforts have created a need for better strategies to functionally categorize newly discovered proteins. To this end, we have employed saturation transfer difference NMR with pools of closely related cofactors, to determine cofactor preferences. This approach works well for dehydrogenases and has also been applied to cyclic nucleotide‐binding proteins. In the latter application, a protein (radial spoke protein‐2, RSP2) that plays a central role in forming the radial spoke of Chlamydomonas reinhardtii flagella was shown to bind cCMP. cCMP‐binding proteins are rare, although previous reports of their presence in sperm and flagella suggest that cCMP may have a more …

An Informatics Search For The Low-Molecular Weight Chromium-Binding Peptide, Deendayal Dinakarpandian, Vincent Morrissette, Shveta Chaudhary, Kambiz Amini, Brian Bennett, J David Van Horn

Physics Faculty Research and Publications

Background

The amino acid composition of a low molecular weight chromium binding peptide (LMWCr), isolated from bovine liver, is reportedly E:G:C:D::4:2:2:2, though its sequence has not been discovered. There is some controversy surrounding the exact biochemical forms and the action of Cr(III) in biological systems; the topic has been the subject of many experimental reports and continues to be investigated. Clarification of Cr-protein interactions will further understanding Cr(III) biochemistry and provide a basis for novel therapies based on metallocomplexes or small molecules.

Results

A genomic search of the non-redundant database for all possible decapeptides of the reported composition yields three …

Private Savings In Transition Economies: Are There Terms Of Trade Shocks?, Abdur Chowdhury

Economics Faculty Research and Publications

Economic agents in the transition economies are subject to tight credit constraints, which are more pronounced during bad state of nature. Thus, adverse shocks to commodity prices in the world market can force them to reduce savings by a larger amount than they would otherwise have. Empirical analysis using a dynamic panel model and data from 21 transition economies confirm that most of the determinants of savings identified in the literature also apply to the transition economies. The transitory component in the terms of trade have a larger positive impact than the permanent component. This reflects the lack of access …

E-Learning In Business, Heidi Schweizer

College of Education Faculty Research and Publications

e-Learning is replacing face-to-face classroom instruction in a growing number of businesses, but what is the prospect for the continued proliferation of e-learning in business? On one hand, the quality of instruction, the cost effectiveness of new technology, a supportive e-learning educational culture, an expansion of the Internet, an increase in online courses, shorter business cycles, mergers, and increasing competition encourage business use of e-learning. On the other hand, employee reticence in using learning technologies, insufficient corporate investment, lack of business-relevant university courses, narrow bandwidth, and Internet access issues are constricting the business use of these technologies.

Structural Conformation And Leaching From In Vitro Aged And Retrieved Invisalign Appliances, Susan Schuster, George Eliades, Spiros Zinelis, Theodore Eliades, T. Gerard Bradley

School of Dentistry Faculty Research and Publications

The objectives of this study were to investigate the structure of Invisalign appliances (Align Technology, Santa Clara, Calif) after intraoral exposure, and to qualitatively and quantitatively characterize the substances leached from the aligners after accelerated in vitro aging. Samples of Invisalign appliances were randomly selected from 10 patients before intraoral placement and after retrieval, and the prepared specimens were subjected to (1) bright-field optical reflection microscopy to study the surface morphology; (2) Fourier transform infrared microspectroscopy to characterize the in vivo changes in molecular composition induced on appliance surfaces, (3) scanning electron microscopy and energy dispersive X-ray microanalysis to identify …

A Tga/Ftir And Mass Spectral Study On The Thermal Degradation Of Bisphenol A Polycarbonate, Bok Nam Jang, Charles A. Wilkie

Chemistry Faculty Research and Publications

The thermal degradation of polycarbonate under nitrogen was studied using TGA/FTIR, GC/MS and LC/MS as a function of mass loss. The gases evolved during degradation were inspected by in situ FTIR and then the evolved products were collected and analysed using FTIR, GC–MS and LC–MS. The structures of the evolved products are assigned on the basis of FTIR and GC/MS results. The main thermal degradation pathways follow chain scission of the isopropylidene linkage, and hydrolysis/alcoholysis and rearrangement of carbonate linkages. In the case of chain scission, it was proposed that methyl scission of isopropylidene occurs first, according to the bond …

Atoms, Complexes, And Demonstration: Posterior Analytics 96b15-25, Owen Goldin

Philosophy Faculty Research and Publications

There is agreement neither concerning the point that is being made in Posterior analytics 96b15-25 nor the issue Aristotle intends to address. There are two major lines of interpretation of this passage. According to one, sketched by Themistius and developed by Philoponus and Eustratius, Aristotle is primarily concerned with determining the definitions of the infimae species that fall under a certain genus. They understand Aristotle as arguing that this requires collating definitional predictions, seeing which are common to which species. Pacius, on the other hand, takes Aristotle to be saying that a genus is studied scientifically through first determining the …

A Whole-Class Support Model For Early Literacy: The Anna Plan, Pamela A. Miles, Kathy W. Stegle, Karen G. Hubbs, Bill Henk, Marla H. Mallette

College of Education Faculty Research and Publications

The Anna Plan is a unique delivery model for enhancing schoolwide literacy instruction in the primary grades. Based on the principles of Reading Recovery and Four Blocks literacy instruction, it provides supplementary reading instruction through the distinctive use of teaching staff. Over six years, it has resulted in sweeping changes in the way literacy instruction occurs as well as noteworthy increases in children's reading abilities. This article gives a brief history of the authors' work within the Anna Plan, explains each of the model's seven tenets, and describes the research base that drives it. The focal point of the article …

Quantitative Models Of The Rat Pulmonary Arterial Tree Morphometry Applied To Hypoxia-Induced Arterial Remodeling, Robert C. Molthen, Kelly L. Karau, Christopher A. Dawson

Biomedical Engineering Faculty Research and Publications

Little is known about the constituent hemodynamic consequences of structural changes that occur in the pulmonary arteries during the onset and progression of pulmonary arterial remodeling. Many disease processes are known to be responsible for vascular remodeling that leads to pulmonary arterial hypertension, cor pulmonale, and death. Histology has been the primary tool for evaluating pulmonary remodeling, but it does not provide information on intact vascular structure or the vessel mechanical properties. This study is an extension of our previous work in which we developed an alternative imaging technique to evaluate pulmonary arterial structure. The lungs from Sprague-Dawley rats were …

Self-Regulation In A Web-Based Course: A Case Study, Joan Whipp, Stephannie Chiarelli

College of Education Faculty Research and Publications

Little is known about how successful students in Web-based courses self-regulate their learning. This descriptive case study used a social cognitive model of self-regulated learning (SRL) to investigate how six graduate students used and adapted traditional SRL strategies to complete tasks and cope with challenges in a Web-based technology course; it also explored motivational and environmental influences on strategy use. Primary data sources were three transcribed interviews with each of the students over the course of the semester, a transcribed interview with the course instructor, and the students’ reflective journals. Archived course documents, including transcripts of threaded discussions and student …

Measurement Of Handle Forces For Crimping Connectors And Cutting Cable In The Electric Power Industry, Richard W. Marklin, Lia Lazuardi, Jeremy R. Wilzbacher

Mechanical Engineering Faculty Research and Publications

Overhead and underground line work in the electric power industry is physically very strenuous and can expose workers to musculoskeletal disorders (MSDs), particularly in the upper extremity. Crimping compression connectors—such as sleeve connectors and lugs—and cutting cables are two of the most frequent tasks that line workers perform. Line workers at many utilities in the US crimp connectors and cut cable with long-handled manual tools. However, the actual magnitude of the forces applied to the handles of these tools is not known. The objectives of this laboratory study were to measure the forces applied to the handles of a manual …

Spotting The Chick: An Essay On Formation In The Context Of Church As Family, Agbonkhianmeghe E. Orobator

Theology Faculty Research and Publications

No abstract provided.

Ic138 Is A Wd-Repeat Dynein Intermediate Chain Required For Light Chain Assembly And Regulation Of Flagellar Bending, Triscia W. Hendrickson, Catherine A. Perrone, Paul Griffin, Kristin Wuichet, Joshua Mueller, Pinfen Yang, Mary E. Porter, Winfield S. Sale

Biological Sciences Faculty Research and Publications

Increased phosphorylation of dynein IC IC138 correlates with decreases in flagellar microtubule sliding and phototaxis defects. To test the hypothesis that regulation of IC138 phosphorylation controls flagellar bending, we cloned the IC138 gene. IC138 encodes a novel protein with a calculated mass of 111 kDa and is predicted to form seven WD-repeats at the C terminus. IC138 maps near the BOP5 locus, and bop5-1 contains a point mutation resulting in a truncated IC138 lacking the C terminus, including the seventh WD-repeat. bop5-1 cells display wild-type flagellar beat frequency but swim slower than wild-type cells, suggesting that bop5-1 is altered in …

Review Of "Modern Social Imaginaries," By Charles Taylor, Philip J. Rossi

Theology Faculty Research and Publications

No abstract provided.

Condition Monitoring Of Squirrel-Cage Induction Motors Fed By Pwm-Based Drives Using A Parameter Estimation Approach, Behrooz Mirafzal, F. Fateh, Chia-Chou Yeh, Richard J. Povinelli, Nabeel Demerdash

Electrical and Computer Engineering Faculty Research and Publications

Abstract:

A rotor condition monitoring technique is presented in this paper based on a parameter estimation approach. In this technique, the stator currents, voltages and motor speed are used as the input signals, where the outputs will be the rotor's inductance, resistance and consequently rotor time constant. This approach is verified by simulation of two different induction motor cases. These simulations are buttressed by experimental data obtained for a 2-hp induction motor in the case of healthy as well as one, three and five rotor bar breakages. In these tests, the induction motor was energized from a PWM-based drive, in …

Conducting Life History Research With Adolescents, Kristin Haglund

College of Nursing Faculty Research and Publications

This article is a commentary on the life history approach, which specifically described aspects of and offered suggestions for conducting life history research with adolescents. The life history approach is well suited to a wide variety of types of health-related inquiry. In this article, the author used an exemplar study to show how well the life history approach was suited for questions regarding adolescent issues. This approach was also a good match for the developmental skills and needs of adolescents. The author uses the strengths and weaknesses of the approach and a detailed example of how she used the life …

The Yeast Rrna Biosynthesis Factor Ebp2p Is Also Required For Efficient Nuclear Division, Costin N. Ionescu, Sofia Origanti, Michael A. Mcalear

Biological Sciences Faculty Research and Publications

Molecular genetic analysis of the yeast Ebp2 protein has revealed that it is an essential, nucleolar protein that functions in the rRNA biosynthesis pathway. Temperature-sensitive ebp2-1 mutants are defective in the processing of the 27 SA precursor rRNA, and the point substitutions that disrupt this activity cluster towards the central, more highly conserved region of the Ebp2 protein. We report here that other ebp2 mutants exhibit deficiencies associated with defects in chromosome segregation. Yeast cells bearing a 50 amino acid C-terminal truncation allele (ebp2ΔC50) display a slow-growth phenotype and exhibit an increased percentage of cells with …

Deadline Analysis Of Interrupt-Driven Software, Dennis Brylow, Jens Palsberg

Mathematics, Statistics and Computer Science Faculty Research and Publications

Real-time, reactive, and embedded systems are increasingly used throughout society (e.g., flight control, railway signaling, vehicle management, medical devices, and many others). For real-time, interrupt-driven software, timely interrupt handling is part of correctness. It is vital for software verification in such systems to check that all specified deadlines for interrupt handling are met. Such verification is a daunting task because of the large number of different possible interrupt arrival scenarios. For example, for a Z86-based microcontroller, there can be up to six interrupt sources and each interrupt can arrive during any clock cycle. Verification of such systems has traditionally relied …

Function Of The Signal Peptide And N- And C-Terminal Propeptides In The Leucine Aminopeptidase From Aeromonas Proteolytica, Krzysztof P. Bzymek, Ventris M. D'Souza, Guanjing Chen, Heidi Campbell, Alice Mitchell, Richard C. Holz

Chemistry Faculty Research and Publications

The leucine aminopeptidase from Aeromonas proteolytica (also known as Vibrio proteolyticus) (AAP) is a metalloenzyme with broad substrate specificity. The open reading frame (ORF) for AAP encodes a 54 kDa enzyme, however, the extracellular enzyme has a molecular weight of 43 kDa. This form of AAP is further processed to a mature, thermostable 32 kDa form but the exact nature of this process is unknown. Over-expression of different forms of AAP in Escherichia coli (with AAP's native leader sequence, with and without the N- and/or C-terminal propeptides, and as fusion protein) has allowed a model for the processing of …

Asymmetric Atp Binding And Hydrolysis Activity Of The Thermus Aquaticus Muts Dimer Is Key To Modulation Of Its Interactions With Mismatched Dna, Edwin Antony, Manju M. Hingorani

Biological Sciences Faculty Research and Publications

Prokaryotic MutS and eukaryotic Msh proteins recognize base pair mismatches and insertions or deletions in DNA and initiate mismatch repair. These proteins function as dimers (and perhaps higher order oligomers) and possess an ATPase activity that is essential for DNA repair. Previous studies of Escherichia coli MutS and eukaryotic Msh2−Msh6 proteins have revealed asymmetry within the dimer with respect to both DNA binding and ATPase activities. We have found the Thermus aquaticus MutS protein amenable to detailed investigation of the nature and role of this asymmetry. Here, we show that (a) in a MutS dimer one subunit (S1) binds nucleotide …

Islamic Revival Among Second-Generation Arab-American Muslims: The American Experience And Globalization Intersect, Louise Cainkar

Social and Cultural Sciences Faculty Research and Publications

This article examines the chain of events that facilitated an Islamic revival among second-generation Arab-American Muslims. Based upon research in metropolitan Chicago, it argues against trends in the literature that described Western-born Muslims as foreigners, immigrants or, worse, anti-Western. Similarly, it argues against setting their religious experiences solely in a domestic context. The article begins by documenting the lack of religious institutions and practices among immigrant Arab Muslims before the 1990s and the limited religious socialization of their American-born children. These conditions emerged in part from secular trends in the immigrants' homelands. By the 1990s, a period of global Islamic …

Love Your Enemies: Life Sciences In The Ecclesial-Based University, M. Therese Lysaught

Theology Faculty Research and Publications

No abstract provided.

On The Philosophical Consistency Of Season Seven; Or, "It's Not About Right, Not About Wrong…", James B. South

Philosophy Faculty Research and Publications

No abstract provided.

Assessing Student Exposure To And Use Of Computer Technologies Through An Examination Of Course Syllabi, Michael B. Madson, Timothy P. Melchert, Joan Whipp

College of Education Faculty Research and Publications

A syllabus analysis instrument was developed to assist program evaluators, administrators and faculty in the identification of skills that students use as they complete their college coursework. While this instrument can be tailored for use with a variety of learning domains, we used it to assess students' use of and exposure to computer technology skills. The reliability and validity of the instrument was examined through an analysis of 88 syllabi from courses within the teacher education program and the core curriculum at a private Midwest US university. Results indicate that the instrument has good inter‐rater reliability and ratings by and …

Admittance Selection For Force-Guided Assembly Of Polygonal Parts Despite Friction, Shuguang Huang, Joseph M. Schimmels

Mechanical Engineering Faculty Research and Publications

An important issue in the development of force guidance assembly strategies is the specification of an appropriate admittance control law. This paper identifies conditions to be satisfied when selecting the appropriate admittance to achieve force-guided assembly of polygonal parts for multipoint contact with friction. These conditions restrict the admittance behavior for each of the various one-point and two-point contact cases and ensure that the motion that results from contact reduces part misalignment for each case. We show that, for bounded friction and part misalignments, if the identified conditions are satisfied for a finite number of contact configurations and friction coefficients, …

Does Gender Make A Difference? Voices Of Male And Female High School Principals, Ellen Eckman

College of Education Faculty Research and Publications

Eckman examines the similarities and differences between how males and females experience the role of being a high school principal. He also provides insights into the high school principalship that can be useful to both males and females who might consider the position as a career choice.

Modulation Of Airway Inflammation By Immunostimulatory Cpg Oligodeoxynucleotides In A Murine Model Of Allergic Aspergillosis, Banani Banerjee, Kevin J. Kelly, Jordan N. Fink, James D. Henderson Jr., Naveen K. Bansal, Viswanath P. Kurup

Mathematics, Statistics and Computer Science Faculty Research and Publications

Allergic aspergillosis is a Th2 T-lymphocyte-mediated pulmonary complication in patients with atopic asthma and cystic fibrosis. Therefore, any therapeutic strategy that selectively inhibits Th2 T-cell activation may be useful in downregulating allergic lung inflammation in asthma. In the present study, we developed a CpG oligodeoxynucleotide (ODN)-based immune intervention of allergic inflammation in a mouse model of allergic aspergillosis. Four different groups of mice were used in a short-term immunization protocol. Three experimental groups of animals (groups 1 to 3) were sensitized with Aspergillus fumigatus antigens. Animals in group 1 were immunized with A. fumigatus antigen alone, while those in group …

Echoing Narratives, Or Chris Anson At Work And Play, Beth Godbee, Beth Burmester, Tanya R. Cochran

English Faculty Research and Publications

No abstract provided.

Adherence Of Prime-Time Television Advertising Disclosures To The “Clear And Conspicuous" Standard: 1990 Vs. 2002, Mariea Grubbs Hoy, J. Craig Andrews

Marketing Faculty Research and Publications

In 1990, one-fourth of all national television commercials contained disclosures, yet none of the disclosures adhered to all of the Federal Trade Commission's "clear and conspicuous" standard (CCS). As a result of marketplace changes and a 2001 Federal Trade Commission and National Advertising Division joint workshop, the authors anticipate an increase in the number of disclosures and greater adherence to the CCS. The authors find a significant increase in disclosure incidence; however, adherence declines or remains unchanged for most individual guidelines. Finally, the authors provide public policy implications and offer suggestions to increase adherence to the CCS.

Public Perceptions Of The Midwest’S Pavements: Policies And Tradeoffs In Pavement Improvement, Richard Robinson, David Kuemmel

Marketing Faculty Research and Publications

With the budget crisis plaguing so many states, pavement management will compete more vigorously for limited funds. Findings reported from a tri-state pooled-fund research project provide insights and guidelines for pavement improvement derived from the perceptions of the driving public. The Phase II responses from statewide surveys in Iowa, Minnesota, and Wisconsin address the topics of perceptions of the State Departments of Transportation (DOTs), pavement repair tradeoffs, and pavement evaluation. The results disclose specific public perceptions of priorities for spending limited highway funds. Implications for pavement management, policy, planning, as well as for marketing to garner additional funds are explored.