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Lactobacillus Metabolite-Mediated Induction Of Bacteriophage, Taylor Miller-Ensminger
Lactobacillus Metabolite-Mediated Induction Of Bacteriophage, Taylor Miller-Ensminger
Master's Theses
Once considered sterile in the absence of an infection, the female urinary tract is home to a diverse community (microbiota) of bacterial species and bacteriophage (phage), viruses that infect bacteria A dominant member of the female urinary microbiota is the bacterial genus Lactobacillus. Several Lactobacillus species are even associated with urinary health. Phage infectious of bacteria in the urinary tract tend to replicate through one of two life cycles: the lytic (active) life cycle or the lysogenic (dormant) life cycle. Temperate phage can switch from the lysogenic life cycle to the lytic life cycle in the presence of an environmental …
Optimizing Clean Catch Urine Collection And Its Applications In Urinary Microbiome Studies, Danielle Johansen
Optimizing Clean Catch Urine Collection And Its Applications In Urinary Microbiome Studies, Danielle Johansen
Master's Theses
Clean catch was developed before the advancement of more sensitive assays for culturing urinary bacteria. I reassessed clean catch, endeavoring to optimize this method. Periurethral swabs and voided urines were routinely collected, while analyzing different aspects of the method. I determined that midstream urine contained the least amount of vulvo-vaginal contamination and that the time of collection did not impact the results. I also determined that the use of antiseptic wipes prior to void increased the level of vulvo-vaginal contamination. A small cohort of females collected daily urines over a 17-day period and kept a strict alcohol diary. Females who …
Characterization Of Inhibitory Molecules Produced By Lactobacillus Crispatus, Giuseppe Anthony Pistone
Characterization Of Inhibitory Molecules Produced By Lactobacillus Crispatus, Giuseppe Anthony Pistone
Master's Theses
Lactobacillus species are widely accepted as beneficial bacteria of the human microbiota1-8. Lactobacilli spp. are well documented to inhibit pathogens by production and secretion of hydrogen peroxide (H2O2), organic acids, and/or proteinaceous bacteriocins into their environment. Most research attributes bactericidal activity of cell free supernatant (CFS) to H2O2 and/or lactic acid. Here, I demonstrate that CFS from a clinical isolate of Lactobacillus crispatus contains a molecule(s) that inhibits uropathogenic Escherichia coli (UPEC) colony formation independently of H2O2 and organic acids. Physiologic concentrations of H2O2 and organic acids produced by L. crispatus do not inhibit UPEC colony formation. Incubation of UPEC …
The Contribution Of The Female Urinary Microbiota To Lower Urinary Tract Symptoms, Travis Kyle Price
The Contribution Of The Female Urinary Microbiota To Lower Urinary Tract Symptoms, Travis Kyle Price
Master's Theses
The recent finding that urine is not sterile raises a lot of questions: first and foremost, what microorganisms are present, and are these microorganisms correlated with clinical urinary symptoms? Recent work on the female urinary microbiota (FUM) found that the communities of microorganisms differ between women with no lower urinary tract symptoms (LUTS) and those with Urgency Urinary Incontinence (UUI) a form of over-active bladder syndrome. Specifically, a diverse or dysbiotic urinary microbiota was found to be associated with symptomatic women. This suggests the possibility that the FUM can be contributing to urinary symptoms. Conversely, it could also suggest that …