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The Effect Of Holding Bovine Oocytes In Follicular Fluid On Subsequent Fertilization And Embryonic Development, Angela Marie Klumpp
The Effect Of Holding Bovine Oocytes In Follicular Fluid On Subsequent Fertilization And Embryonic Development, Angela Marie Klumpp
LSU Master's Theses
The objective of Experiment 1 was to determine the effect of bovine follicular fluid (bFF) on nuclear maturation. Treatment A (Control) oocytes were stained with Hoechst-33342 immediately after aspiration from follicles, whereas, oocytes in Treatment B were held in bFF for 12 hours at 38¢ªC and then stained to determine nuclear status. No significant difference was detected between treatment groups. Results indicate that bFF inhibits resumption of meiosis. The objective of Experiment 2 was to determine the effect of bFF on embryonic development. Oocytes in Treatment A (Control) were placed into in vitro maturation (IVM) for 22 hours followed by …