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Louisiana State University

LSU Doctoral Dissertations

Theses/Dissertations

Molecular markers

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Phenotypic And Genotypic Characterization Of Grain Quality In Rice And Implications For Breeding, Raul A. Guerra-Lacayo Jun 2023

Phenotypic And Genotypic Characterization Of Grain Quality In Rice And Implications For Breeding, Raul A. Guerra-Lacayo

LSU Doctoral Dissertations

Rice will continue to be a staple food for the foreseeable future, and plant breeding can foment the resilience of this crop. Applied breeding programs with clear objectives can effectively develop varieties that satisfy stakeholder needs. The majority of U.S.-produced rice is destined to export markets, and Latin America is the largest importer of U.S. rice, receiving 59% of total exports in 2021. Since 2010, Latin American importers voiced concerns about the quality of U.S. rice, mainly the high chalk content of milled rice and the stickiness of cooked rice. To address these issues, the objectives of this project were: …


Development Of Sheath Blight-Resistant Breeding Lines For Southern U.S. Environments And Morphological And Genetic Survey Of Giant Salvinia Populations In Louisiana And Texas, Dominique Clark Galam Mar 2019

Development Of Sheath Blight-Resistant Breeding Lines For Southern U.S. Environments And Morphological And Genetic Survey Of Giant Salvinia Populations In Louisiana And Texas, Dominique Clark Galam

LSU Doctoral Dissertations

The southern US environment is a very conducive environment for agriculture and fisheries. Rice farming, shrimping and water related activities help drive the local economy. However, there are several factors that impede the success of these activities. Sheath blight (SB) disease caused by Rhizoctonia solani is one of the major biotic constraints to high grain yield and quality for most commercial U.S. rice varieties. Although different breeding lines with high levels of "partial resistance" have been developed none has been used directly as a commercial variety. The first research objective of this research was to identify and develop advanced breeding …


Pathogenicity And Reproduction Of Isolates Of Reniform Nematode, Rotylenchulus Reniformis, From Louisiana On Soybean And Utility Of Single Nucleotide Polymorphisms To Evaluate Genetic Variability, Herath Mudiyanselage Kularathna Nov 2017

Pathogenicity And Reproduction Of Isolates Of Reniform Nematode, Rotylenchulus Reniformis, From Louisiana On Soybean And Utility Of Single Nucleotide Polymorphisms To Evaluate Genetic Variability, Herath Mudiyanselage Kularathna

LSU Doctoral Dissertations

Experiments were conducted to evaluate soybean, Glycine max (L.) Merr., responses to indigenous isolates of the reniform nematode (Rotylenchulus reniformis) in Louisiana and to understand the genetic variability of these native isolates. Microplot and greenhouse experiments were conducted to evaluate the comparative reproduction and pathogenicity of single egg-mass populations of R. reniformis isolated from West Carroll (WC), Rapides, Tensas and Morehouse (MOR) parishes of Louisiana. Data from full-season microplot trials, displayed significant differences in reproduction and pathogenicity of the nematode with the commercial soybean cultivars REV 56R63, Pioneer P54T94R, and Dyna-Gro 39RY57. Significantly low population density was observed …


Development Of Functional Markers For Resistance To Leaf Scald In Sugarcane, Andres Felipe Gutierrez Viveros Jan 2016

Development Of Functional Markers For Resistance To Leaf Scald In Sugarcane, Andres Felipe Gutierrez Viveros

LSU Doctoral Dissertations

Leaf scald, caused by Xanthomonas albilineans, is a major sugarcane disease worldwide. The disease is managed primarily with resistant cultivars obtained through classical breeding; however, the erratic symptom expression hinders the reliability and reproducibility of the selection process. The development of molecular markers associated with incompatible/compatible reaction can overcome this limitation. Suppression subtractive hybridization (SSH) and quantitative trait locus (QTL) mapping were the strategies used to find leaf scald resistance-associated genes and molecular markers in sugarcane. SSH results showed that genes involved in signal perception and transduction, and DNA binding, were highly expressed in the resistant clone LCP 85-384 compared …


Development Of Disease Resistant Rice Using Whole Genome Sequencing And Standard Breeding Methods, Yamid Sanabria Gongora Jan 2015

Development Of Disease Resistant Rice Using Whole Genome Sequencing And Standard Breeding Methods, Yamid Sanabria Gongora

LSU Doctoral Dissertations

Cultivated rice is the most important staple crop in the world, but diseases cause substantial losses in grain yield and quality. Sheath blight disease caused by the fungus Rhizoctonia solani is the second most important disease in rice. Most U.S varieties are tropical japonica type, but known sources of resistance in this subspecies are rare. Silva et al. (2012) identified candidate SNP associated with resistance to sheath blight by whole genome sequencing. The objectives of this study were to develop SNP-based markers from the information reported by Silva et al. (2012), to validate the markers by selective genotyping in the …


Genetics And Genomics Studies Of Rice Disease Resistance And Development Of Alternative Disease Management Methods For Bacterial Panicle Blight And Sheath Blight, Bishnu Kumar Shrestha Jan 2014

Genetics And Genomics Studies Of Rice Disease Resistance And Development Of Alternative Disease Management Methods For Bacterial Panicle Blight And Sheath Blight, Bishnu Kumar Shrestha

LSU Doctoral Dissertations

Bacterial panicle blight (BPB) and sheath blight (SB), caused by the bacteria Burkholderia glumae and B. gladioli, and the fungus Rhizoctonia solani, respectively, are two major rice diseases in southern rice growing regions of US. No completely resistant rice cultivars have been identified for these diseases. However, a medium-grain cultivar, Jupiter, showed partial resistance to BPB. In order to understand the mechanisms of rice resistance against BPB and SB, rice genetics and genomics studies have been conducted. Alternative methods to suppress BPB and SB were also studied. Broad-sense heritability and correlations were calculated for the traits, BPB and SB disease …