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Louisiana State University

LSU Doctoral Dissertations

Theses/Dissertations

2004

In vitro fertilization

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Developing Embryo Technologies For The Eland Antelope (Taurotragus Oryx), Gemechu G. Wirtu Jan 2004

Developing Embryo Technologies For The Eland Antelope (Taurotragus Oryx), Gemechu G. Wirtu

LSU Doctoral Dissertations

Assisted reproductive technologies developed in domestic cattle serve as a starting point in similar studies on nondomestic bovids. The common eland is a useful model species for studies on rare tragelaphine antelopes. In Chapter 3 of the present study, effects of components/attributes of protein-free embryo culture media on the in vitro development of in vitro-derived bovine embryos were evaluated. A 2 x 2 factorial study comparing effects of groups of amino acids (20aa or 11aa) in two base media (modified KSOM or BM-3) demonstrated that amino acids and base medium affected embryonic development. A subsequent 7 x 2 factorial experiment …


The Chick Embryo Amnion As An In Vitro Culture System For Ivf And Nt Embryos, Tonya Renea Davidson Jan 2004

The Chick Embryo Amnion As An In Vitro Culture System For Ivf And Nt Embryos, Tonya Renea Davidson

LSU Doctoral Dissertations

Calving rates are significantly reduced following in vitro production of embryos. Thus, if a technique could be developed that would increase calving rates by as little as one viable offspring, significant research advances could be made. Therefore, in a series of experiments, the efficiency and quality of culturing IVP bovine embryos in the amnion of a domestic chicken egg was tested. In Experiment I, by culturing IVP bovine embryos in the chick amnion (day 4 to 7 of incubation) it was discovered that there was no significant difference in blastocyst rates compared with controls. In Experiment II, it was shown …