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Initiation Of Apoptosis And Autophagy By Photodynamic Therapy, David Kessel, M. Graça H. Vicente, John J. Reiners
Initiation Of Apoptosis And Autophagy By Photodynamic Therapy, David Kessel, M. Graça H. Vicente, John J. Reiners
Faculty Publications
Background and Objectives: This study was designed to examine modes of cell death after photodynamic therapy (PDT). Study Design: Murine leukemia L1210 cells and human prostate Bax-deficient DU145 cells were examined after PDT-induced photodamage to the endoplasmic reticulum (ER). Phase contrast, fluorescence and electron microscopy were used to identify changes in cellular morphology, chromatin condensation, loss of mitochondrial membrane potential, and formation of phagolysosomes. Western blots were used to assess the processing of LC3-I to LC3-II, a marker for autophagy. Inhibitors of apoptosis and/or autophagy were used to delineate the contributions of the two pathways to the effects of PDT. …
Initiation Of Apoptosis And Autophagy By Photodynamic Therapy, David Kessel, M. Graça H. Vicente, John J. Reiners
Initiation Of Apoptosis And Autophagy By Photodynamic Therapy, David Kessel, M. Graça H. Vicente, John J. Reiners
Faculty Publications
This study was designed to examine modes of cell death after photodynamic therapy (PDT). Murine leukemia L1210 cells and human prostate Box-deficient DU-145 cells were examined after PDT-induced photodamage to the endoplasmic reticulum (ER). Previous studies indicated that this treatment resulted in a substantial loss of Bcl-2 function. Both apoptosis and autophagy occurred in L1210 cells after ER photodamage with the latter predominating after 24 hr. These processes were characterized by altered cellular morphology, chromatin condensation, loss of mitochondrial membrane potential and formation of vacuoles containing cytosolic components. Western blots demonstrated processing of LC3-I to LC3-II, a marker for autophagy. …