Open Access. Powered by Scholars. Published by Universities.®

Digital Commons Network

Open Access. Powered by Scholars. Published by Universities.®

Articles 1 - 2 of 2

Full-Text Articles in Entire DC Network

An Inexpensive, Accurate Method For Measuring Leaf Area And Defoliation Through Digital Image Analysis, Matthew E. O'Neal, Douglas A. Landis, Rufus Isaacs Dec 2002

An Inexpensive, Accurate Method For Measuring Leaf Area And Defoliation Through Digital Image Analysis, Matthew E. O'Neal, Douglas A. Landis, Rufus Isaacs

Matthew E. O'Neal

We report a protocol using a common desk-top scanner and public domain software for measuring existing leaf area and leaf area removed as a result of herbivory. We compared the accuracy and precision of this method to that of a standard leaf area meter. Both methods were used to measure metal disks of a known area, the area of soybean (Glycine max L.) leaves, and the area removed by simulating leaf feeding with a hole-punch. We varied the amount of injury across a low, medium, and high degree of simulated feeding. The mean area of 10 cm2 and 50 cm2 ...


Microscopy Of A Cytoplasmic Male-Sterile Soybean From An Interspecific Cross Between Glycine Max And G. Soja (Leguminosae), Marianne B. Smith, Reid G. Palmer, Harry T. Horner Mar 2002

Microscopy Of A Cytoplasmic Male-Sterile Soybean From An Interspecific Cross Between Glycine Max And G. Soja (Leguminosae), Marianne B. Smith, Reid G. Palmer, Harry T. Horner

Botany Publication and Papers

Cytoplasmic male sterility has been found independently in soybean three times since 1995, but no microscopic investigation has been published. The purpose of this microscopic study was to establish the developmental sequence leading to sterility in a cytoplasmic male-sterile soybean line that has been found to be stable under all environmental conditions tested and to demarcate the temporal and spatial parameters that result in degeneration of the microspores and pollen grains. Light microscopy showed an abnormal development and/or premature degeneration of the tapetum after meiosis II, but some pollen grains persisted until after microspore mitosis. The pollen grains never ...