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Effects Of Rag1 On The Preference And Performance Of Soybean Defoliators, Robert F. Bruner, Erin W. Hodgson, Aaron J. Gassmann Dec 2013

Effects Of Rag1 On The Preference And Performance Of Soybean Defoliators, Robert F. Bruner, Erin W. Hodgson, Aaron J. Gassmann

Entomology Publications

The Rag1 gene confers antibiotic resistance to soybean aphid, Aphis glycines Matsumura (Hemiptera: Aphididae), and in 2010, varieties expressing Rag1 were released for commercial use in the United States. We do not know how Rag1 varieties will influence the broader community of defoliating insects that inhabit soybean fields. In 2010 and 2011, the preference and performance of pest insects that defoliate soybeans [Glycines max (L.) Merr] were tested using Rag1 and aphidsusceptible varieties. Three coleopterans and four lepidopterans were used: northern corn rootworm, Diabrotica barberi Smith & Lawrence (Coleoptera: Chrysomelidae); southern corn rootworm, Diabrotica undecimpunctata howardi Barber (Coleoptera: Chrysomelidae); bean leaf beetle, Ceratoma trifurcata ...


Investigation Of The Arabidopsis Nonhost Resistance Mechanism Against The Soybean Pathogen, Phytophthora Sojae, Rishi Sumit Jan 2013

Investigation Of The Arabidopsis Nonhost Resistance Mechanism Against The Soybean Pathogen, Phytophthora Sojae, Rishi Sumit

Graduate Theses and Dissertations

Nonhost resistance (NHR) provides immunity to all members of a plant species against all isolates of a microorganism that is pathogenic to other plant species. Three Arabidopsis thaliana PEN (penetration deficient) genes, PEN1, 2 and 3 have been shown to provide prehaustorial NHR against the barley pathogen Blumeria graminis f. sp. hordei. Arabidopsis pen1-1 mutant is penetrated by the hemibiotrophic oomycete pathogen, Phytophthora sojae that causes root and stem rot disease in soybean. The P. sojae susceptible (pss) 1 mutant is infected by both P. sojae and the hemibiotrophic fungal pathogen, Fusarium virguliforme that causes sudden death syndrome in soybean ...


Transcriptome Analyses And Virus Induced Gene Silencing Identify Genes In The Rpp4 -Mediated Asian Soybean Rust Resistance Pathway, Aguida A.M.P. Morales, Jamie A. O'Rourke, Martijn Van De Mortel, Katherine T. Scheider, Timothy J. Bancroft, Aluizio Borem, Rex T. Nelson, Daniel S. Nettleton, Thomas J. Baum, Randy C. Shoemaker, Reid D. Frederick, Ricardo V. Abdelnoor, Kerry F. Pedley, Steven A. Whitham, Michelle Graham Jan 2013

Transcriptome Analyses And Virus Induced Gene Silencing Identify Genes In The Rpp4 -Mediated Asian Soybean Rust Resistance Pathway, Aguida A.M.P. Morales, Jamie A. O'Rourke, Martijn Van De Mortel, Katherine T. Scheider, Timothy J. Bancroft, Aluizio Borem, Rex T. Nelson, Daniel S. Nettleton, Thomas J. Baum, Randy C. Shoemaker, Reid D. Frederick, Ricardo V. Abdelnoor, Kerry F. Pedley, Steven A. Whitham, Michelle Graham

Plant Pathology and Microbiology Publications

Rpp4 (Resistance to Phakopsora pachyrhizi 4) confers resistance to Phakopsora pachyrhizi Sydow, the causal agent of Asian soybean rust (ASR). By combining expression profiling and virus induced gene silencing (VIGS), we are developing a genetic framework for Rpp4-mediated resistance. We measured gene expression in mock-inoculated and P. pachyrhizi-infected leaves of resistant soybean accession PI459025B (Rpp4) and the susceptible cultivar (Williams 82) across a 12-day time course. Unexpectedly, two biphasic responses were identified. In the incompatible reaction, genes induced at 12 h after infection (hai) were not differentially expressed at 24 hai, but were induced at 72 hai. In contrast, genes ...


A Virus-Induced Gene Silencing Method To Study Soybean Cyst Nematode Parasitism In Glycine Max, Pramod K. Kandoth, Robert Heinz, Gregory Yeckel, Nathan W. Gross, Parijat S. Juvale, John H. Hill, Steven A. Whitham, Thomas J. Baum, Melissa G. Mitchum Jan 2013

A Virus-Induced Gene Silencing Method To Study Soybean Cyst Nematode Parasitism In Glycine Max, Pramod K. Kandoth, Robert Heinz, Gregory Yeckel, Nathan W. Gross, Parijat S. Juvale, John H. Hill, Steven A. Whitham, Thomas J. Baum, Melissa G. Mitchum

Plant Pathology and Microbiology Publications

Background

Bean pod mottle virus (BPMV) based virus-induced gene silencing (VIGS) vectors have been developed and used in soybean for the functional analysis of genes involved in disease resistance to foliar pathogens. However, BPMV-VIGS protocols for studying genes involved in disease resistance or symbiotic associations with root microbes have not been developed.

Findings

Here we describe a BPMV-VIGS protocol suitable for reverse genetic studies in soybean roots. We use this method for analyzing soybean genes involved in resistance to soybean cyst nematode (SCN). A detailed SCN screening pipeline is described.

Conclusions

The VIGS method described here provides a new tool ...