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Iowa State University

Soybean

Plant Pathology

Plant Pathology and Microbiology Publications

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Targeted Suppression Of Soybean Bag6‐Induced Cell Death In Yeast By Soybean Cyst Nematode Effectors, Jianying Wang, Greg Yeckel, Pramod K. Kandoth, Lakmini Wasala, Richard S. Hussey, Eric L. Davis, Thomas J. Baum, Melissa G. Mitchum Jan 2020

Targeted Suppression Of Soybean Bag6‐Induced Cell Death In Yeast By Soybean Cyst Nematode Effectors, Jianying Wang, Greg Yeckel, Pramod K. Kandoth, Lakmini Wasala, Richard S. Hussey, Eric L. Davis, Thomas J. Baum, Melissa G. Mitchum

Plant Pathology and Microbiology Publications

While numerous effectors that suppress plant immunity have been identified from bacteria, fungi, and oomycete pathogens, relatively little is known for nematode effectors. Several dozen effectors have been reported from the soybean cyst nematode (SCN). Previous studies suggest that a hypersensitive response‐like programmed cell death is triggered at nematode feeding sites in soybean during an incompatible interaction. However, virulent SCN populations overcome this incompatibility using unknown mechanisms. A soybean BAG6 (Bcl‐2 associated anthanogene 6) gene previously reported by us to be highly up‐regulated in degenerating feeding sites induced by SCN in a resistant soybean line was attenuated ...


Screening Soybean Cyst Nematode Effectors For Their Ability To Suppress Plant Immunity, Gennady Pogorelko, Jianying Wang, Parijat S. Juvale, Melissa G. Mitchum, Thomas J. Baum Jan 2020

Screening Soybean Cyst Nematode Effectors For Their Ability To Suppress Plant Immunity, Gennady Pogorelko, Jianying Wang, Parijat S. Juvale, Melissa G. Mitchum, Thomas J. Baum

Plant Pathology and Microbiology Publications

The soybean cyst nematode (SCN), Heterodera glycines , is one of the most destructive pathogens of soybeans. SCN is an obligate and sedentary parasite that transforms host plant root cells into an elaborate permanent feeding site, a syncytium. Formation and maintenance of a viable syncytium is an absolute requirement for nematode growth and reproduction. In turn, sensing pathogen attack, plants activate defence responses and may trigger programmed cell death at the sites of infection. For successful parasitism, H. glycines must suppress these host defence responses to establish and maintain viable syncytia. Similar to other pathogens, H. glycines engages in these molecular ...


Homeostasis In The Soybean Mirna396–Grf Network Is Essential For Productive Soybean Cyst Nematode Infections, Jason B. Noon, Tarek Hewezi, Thomas J. Baum Jan 2019

Homeostasis In The Soybean Mirna396–Grf Network Is Essential For Productive Soybean Cyst Nematode Infections, Jason B. Noon, Tarek Hewezi, Thomas J. Baum

Plant Pathology and Microbiology Publications

Heterodera glycines, the soybean cyst nematode, penetrates soybean roots and migrates to the vascular cylinder where it forms a feeding site called the syncytium. MiRNA396 (miR396) targets growth-regulating factor (GRF) genes, and the miR396–GRF1/3 module is a master regulator of syncytium development in model cyst nematode H. schachtii infection of Arabidopsis. Here, we investigated whether this regulatory system operates similarly in soybean roots and is likewise important for H. glycines infection. We found that a network involving nine MIR396 and 23 GRF genes is important for normal development of soybean roots and that GRF function is specified in ...


Virus-Induced Gene Silencing And Transient Gene Expression In Soybean (Glycine Max) Using Bean Pod Mottle Virus Infectious Clones, Steven A. Whitham, Lori M. Lincoln, R. V. Chowda-Reddy, Jaime D. Dittman, Jamie A. O'Rourke, Michelle A. Graham Jun 2016

Virus-Induced Gene Silencing And Transient Gene Expression In Soybean (Glycine Max) Using Bean Pod Mottle Virus Infectious Clones, Steven A. Whitham, Lori M. Lincoln, R. V. Chowda-Reddy, Jaime D. Dittman, Jamie A. O'Rourke, Michelle A. Graham

Plant Pathology and Microbiology Publications

Virus-induced gene silencing (VIGS) is a powerful and rapid approach for determining the functions of plant genes. The basis of VIGS is that a viral genome is engineered so that it can carry fragments of plant genes, typically in the 200 to 300 base pair size range. The recombinant viruses are used to infect experimental plants, and wherever the virus invades, the target gene or genes will be silenced. VIGS is thus transient, and in the span of a few weeks, it is possible to design VIGS constructs and then generate loss-of-function phenotypes through RNA silencing of the target genes ...


Identification Of Candidate Genes Involved In Early Iron Deficiency Chlorosis Signaling In Soybean (Glycine Max) Roots And Leaves, Adrienne N. Moran-Lauter, Gregory Allan Peiffer, Tengfei Yin, Steven A. Whitham, Dianne Cook, Randy Shoemaker, Michelle Graham Jan 2014

Identification Of Candidate Genes Involved In Early Iron Deficiency Chlorosis Signaling In Soybean (Glycine Max) Roots And Leaves, Adrienne N. Moran-Lauter, Gregory Allan Peiffer, Tengfei Yin, Steven A. Whitham, Dianne Cook, Randy Shoemaker, Michelle Graham

Plant Pathology and Microbiology Publications

Background

Iron is an essential micronutrient for all living things, required in plants for photosynthesis, respiration and metabolism. A lack of bioavailable iron in soil leads to iron deficiency chlorosis (IDC), causing a reduction in photosynthesis and interveinal yellowing of leaves. Soybeans (Glycine max (L.) Merr.) grown in high pH soils often suffer from IDC, resulting in substantial yield losses. Iron efficient soybean cultivars maintain photosynthesis and have higher yields under IDC-promoting conditions than inefficient cultivars.

Results

To capture signaling between roots and leaves and identify genes acting early in the iron efficient cultivar Clark, we conducted a RNA-Seq study ...


Transcriptome Analyses And Virus Induced Gene Silencing Identify Genes In The Rpp4 -Mediated Asian Soybean Rust Resistance Pathway, Aguida A.M.P. Morales, Jamie A. O'Rourke, Martijn Van De Mortel, Katherine T. Scheider, Timothy J. Bancroft, Aluizio Borem, Rex T. Nelson, Daniel S. Nettleton, Thomas J. Baum, Randy C. Shoemaker, Reid D. Frederick, Ricardo V. Abdelnoor, Kerry F. Pedley, Steven A. Whitham, Michelle Graham Jan 2013

Transcriptome Analyses And Virus Induced Gene Silencing Identify Genes In The Rpp4 -Mediated Asian Soybean Rust Resistance Pathway, Aguida A.M.P. Morales, Jamie A. O'Rourke, Martijn Van De Mortel, Katherine T. Scheider, Timothy J. Bancroft, Aluizio Borem, Rex T. Nelson, Daniel S. Nettleton, Thomas J. Baum, Randy C. Shoemaker, Reid D. Frederick, Ricardo V. Abdelnoor, Kerry F. Pedley, Steven A. Whitham, Michelle Graham

Plant Pathology and Microbiology Publications

Rpp4 (Resistance to Phakopsora pachyrhizi 4) confers resistance to Phakopsora pachyrhizi Sydow, the causal agent of Asian soybean rust (ASR). By combining expression profiling and virus induced gene silencing (VIGS), we are developing a genetic framework for Rpp4-mediated resistance. We measured gene expression in mock-inoculated and P. pachyrhizi-infected leaves of resistant soybean accession PI459025B (Rpp4) and the susceptible cultivar (Williams 82) across a 12-day time course. Unexpectedly, two biphasic responses were identified. In the incompatible reaction, genes induced at 12 h after infection (hai) were not differentially expressed at 24 hai, but were induced at 72 hai. In contrast, genes ...


A Virus-Induced Gene Silencing Method To Study Soybean Cyst Nematode Parasitism In Glycine Max, Pramod K. Kandoth, Robert Heinz, Gregory Yeckel, Nathan W. Gross, Parijat S. Juvale, John H. Hill, Steven A. Whitham, Thomas J. Baum, Melissa G. Mitchum Jan 2013

A Virus-Induced Gene Silencing Method To Study Soybean Cyst Nematode Parasitism In Glycine Max, Pramod K. Kandoth, Robert Heinz, Gregory Yeckel, Nathan W. Gross, Parijat S. Juvale, John H. Hill, Steven A. Whitham, Thomas J. Baum, Melissa G. Mitchum

Plant Pathology and Microbiology Publications

Background

Bean pod mottle virus (BPMV) based virus-induced gene silencing (VIGS) vectors have been developed and used in soybean for the functional analysis of genes involved in disease resistance to foliar pathogens. However, BPMV-VIGS protocols for studying genes involved in disease resistance or symbiotic associations with root microbes have not been developed.

Findings

Here we describe a BPMV-VIGS protocol suitable for reverse genetic studies in soybean roots. We use this method for analyzing soybean genes involved in resistance to soybean cyst nematode (SCN). A detailed SCN screening pipeline is described.

Conclusions

The VIGS method described here provides a new tool ...


Studies On Seed Colouring In Soybean And Tomato, Vilas A. Tonapi, P. Harinath Babu, S. Varanavasiappan, Ch Ravinder Reddy, Shrishail S. Navi, N. Seetharama Jan 2006

Studies On Seed Colouring In Soybean And Tomato, Vilas A. Tonapi, P. Harinath Babu, S. Varanavasiappan, Ch Ravinder Reddy, Shrishail S. Navi, N. Seetharama

Plant Pathology and Microbiology Publications

The effects of seed colouring using 25 dyes (i.e. 20 chemical and 5 natural dyes) on the quality of soyabean (cv. JS 335) and tomato (cv. Pusa Ruby) seeds were studied in the laboratory. Data were recorded for seed germination percentage, root and shoot length, whole seedling length, dry weight of seedling, vigour index, speed of germination and electrical conductivity. Rhodamine-B, Fast green and Malachite green for soyabean and Rhodamine-B and Fast green for tomato were the best dyes for seed colouring.


Expression Of An Arabidopsis Phosphoglycerate Mutase Homologue Is Localized To Apical Meristems, Regulated By Hormones, And Induced By Sedentary Plant-Parasitic Nematodes, Mitra Mazarei, Kristen A. Lennon, David P. Puthoff, Steven R. Rodermel, Thomas J. Baum Nov 2003

Expression Of An Arabidopsis Phosphoglycerate Mutase Homologue Is Localized To Apical Meristems, Regulated By Hormones, And Induced By Sedentary Plant-Parasitic Nematodes, Mitra Mazarei, Kristen A. Lennon, David P. Puthoff, Steven R. Rodermel, Thomas J. Baum

Plant Pathology and Microbiology Publications

We previously isolated a partial soybean cDNA clone whose transcript abundance is increased upon infection by the sedentary, endoparasitic soybean cyst nematode Heterodera glycines. We now isolated the corresponding full-length cDNA and determined that the predicted gene product was similar to the group of cofactor-dependent phosphoglycerate mutase/bisphosphoglycerate mutase enzymes (PGM/bPGM; EC 5.4.2.1/5.4.2.4). We designated the corresponding soybean gene GmPGM. PGM and bPGM are key catalysts of glycolysis that have been well characterized in animals but not plants. Using the GmPGM cDNA sequence, we identified a homologous Arabidopsis thaliana gene, which we ...


Influence Of Herbicide Application To Soybeans On Soybean Cyst Nematode Egg Hatching, Brian C. Levene, Michael D. K. Owen, Gregory L. Tylka Sep 1998

Influence Of Herbicide Application To Soybeans On Soybean Cyst Nematode Egg Hatching, Brian C. Levene, Michael D. K. Owen, Gregory L. Tylka

Plant Pathology and Microbiology Publications

The hatching of Heterodera glycines eggs in soybean root exudates collected after postemergence application of three herbicides, and the hatching potential of H. glycines eggs from females feeding on herbicide-treated plants, were measured in vitro. Hatching in all root exudate solutions (RES) was greater than in deionized water but less than in 0.003 M ZnSO[sub4] solution. Filtering RES with a 0.22-[mu]m-filter increased H. glycines hatching in RES. Application of acifluorfen, bentazon, and lactofen to foliage of soybean plants inhibited hatching of H. glycines eggs from the same plants. Hatching in RES from the different herbicide-treated ...


Responses Of Heterodera Glycines Populations To A Postemergence Herbicide Mixture And Simulated Insect Defoliation, J. A. Browde, G. L. Tylka, L. P. Pedigo, M. D. K. Owen Dec 1994

Responses Of Heterodera Glycines Populations To A Postemergence Herbicide Mixture And Simulated Insect Defoliation, J. A. Browde, G. L. Tylka, L. P. Pedigo, M. D. K. Owen

Plant Pathology and Microbiology Publications

Effects of a mixture of the postemergence herbicides acifluorfen and bentazon, and simulated defoliation expected from green cloverworm on population densities of Heterodera glycines were determined in field plots in Iowa. The herbicide mixture and defoliation each suppressed soybean growth. Population densities of H. glycines were generally lower in herbicide-treated than untreated plots. Population densities of the nematode were unaffected by defoliation in 1988 and 1990-91, but were increased by the treatment in 1989. Key words: acifluorfen, bentazon, defoliation, Glycine max, green cloverworm, herbicide, Heterodera glycines, nematode, Plathypena scabra, postemergence herbicides, soybean, soybean cyst nematode.