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Iowa State University

Soybean

Plant Biology

2016

Articles 1 - 2 of 2

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Virus-Induced Gene Silencing And Transient Gene Expression In Soybean (Glycine Max) Using Bean Pod Mottle Virus Infectious Clones, Steven A. Whitham, Lori M. Lincoln, R. V. Chowda-Reddy, Jaime D. Dittman, Jamie A. O'Rourke, Michelle A. Graham Jun 2016

Virus-Induced Gene Silencing And Transient Gene Expression In Soybean (Glycine Max) Using Bean Pod Mottle Virus Infectious Clones, Steven A. Whitham, Lori M. Lincoln, R. V. Chowda-Reddy, Jaime D. Dittman, Jamie A. O'Rourke, Michelle A. Graham

Plant Pathology and Microbiology Publications

Virus-induced gene silencing (VIGS) is a powerful and rapid approach for determining the functions of plant genes. The basis of VIGS is that a viral genome is engineered so that it can carry fragments of plant genes, typically in the 200 to 300 base pair size range. The recombinant viruses are used to infect experimental plants, and wherever the virus invades, the target gene or genes will be silenced. VIGS is thus transient, and in the span of a few weeks, it is possible to design VIGS constructs and then generate loss-of-function phenotypes through RNA silencing of the target genes ...


Microscopic Characterization Of A Transposon-Induced Male-Sterile, Female-Sterile Mutant In Glycine Max L., Katherine A. Thilges Jan 2016

Microscopic Characterization Of A Transposon-Induced Male-Sterile, Female-Sterile Mutant In Glycine Max L., Katherine A. Thilges

Graduate Theses and Dissertations

A male-sterile, female-sterile mutant was identified in a transposon-tagging study in soybean (Glycine max L.). This mutant displayed abnormalities in both micro- and mega-sporogenesis, as well as gametogenesis. Vegetatively, the mutant showed no visible differences from the wild-type phenotype. Analyses of male meiotic chromosomes were done to better understand any issues that could occur to cause sterility. Wild-type and mutant anthers and ovules were cleared throughout their development, followed by confocal scanning laser microscopy to look for any abnormalities, and to determine the timing of abortion in both the male and female organs in the mutant. Additionally, scanning electron microscopy ...