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Iowa State University

Soybean

Plant Biology

Theses/Dissertations

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Microscopic Characterization Of A Transposon-Induced Male-Sterile, Female-Sterile Mutant In Glycine Max L., Katherine A. Thilges Jan 2016

Microscopic Characterization Of A Transposon-Induced Male-Sterile, Female-Sterile Mutant In Glycine Max L., Katherine A. Thilges

Graduate Theses and Dissertations

A male-sterile, female-sterile mutant was identified in a transposon-tagging study in soybean (Glycine max L.). This mutant displayed abnormalities in both micro- and mega-sporogenesis, as well as gametogenesis. Vegetatively, the mutant showed no visible differences from the wild-type phenotype. Analyses of male meiotic chromosomes were done to better understand any issues that could occur to cause sterility. Wild-type and mutant anthers and ovules were cleared throughout their development, followed by confocal scanning laser microscopy to look for any abnormalities, and to determine the timing of abortion in both the male and female organs in the mutant. Additionally, scanning electron microscopy ...


A Type Ii Metacaspase Interacts With Rps1-K-2 In Soybean And Analysis Of The Soybean Metacaspase Gene Family, James Andrew Baskett Jan 2012

A Type Ii Metacaspase Interacts With Rps1-K-2 In Soybean And Analysis Of The Soybean Metacaspase Gene Family, James Andrew Baskett

Graduate Theses and Dissertations

Soybean suffers from the root and stem rot disease caused by the oomycete pathogen, Phytophthora sojae. A series of Rps genes confer Phytophthora resistance, and have been utilized in fighting this serious disease in soybean. Earlier we reported the cloning of the coiled coil-nucleotide binding-leucine rich repeat gene, Rps1-k-2. Here we report the identification of a type II metacaspase, Gmmc11 that interacted with the coiled-coil (CC) domain of Rps1-k-2 in vivo in yeast. The interaction was verified by conducting in vitro pull down assays in unpublished work. RNA interference of Gmmc11 in cotyledons resulted in loss of Phytophthora resistance. Gmmc11 ...


Mycorrhizal Fungi-Bradyrhizobium-Soybean Tripartite Symbiosis In Iowa Soils , Samina Khalil Jan 1991

Mycorrhizal Fungi-Bradyrhizobium-Soybean Tripartite Symbiosis In Iowa Soils , Samina Khalil

Retrospective Theses and Dissertations

Soybean [Glycine max (L.) Merr.] makes use of two important symbiotic microbial systems: the rhizobial system, in which Bradyrhizobium japonicum fix atmospheric N2 in root nodules, and the vesicular-arbuscular mycorrhizal system, in which VAM fungi increase uptake of phosphate and/or other nutrients from the soil or improve plant drought tolerance. Research has confirmed the advantage of bradyrhizobial and mycorrhizal associations in N and P deficient soils; however, these associations may be inhibitory, or have little effect on plant growth in well-fertilized soils. In Iowa over the past 30-40 years, farmers have been adding large quantities of fertilizers to get ...


Effect Of Growth Regulators On Reproduction In Soybeans , Howard A. L. Greer Jan 1964

Effect Of Growth Regulators On Reproduction In Soybeans , Howard A. L. Greer

Retrospective Theses and Dissertations

No abstract provided.


Floral Induction In Soja Max , John Edwin Fisher Jan 1953

Floral Induction In Soja Max , John Edwin Fisher

Retrospective Theses and Dissertations

No abstract provided.


Translocation In Soybean Plants , Leo Preston Vernon Jan 1951

Translocation In Soybean Plants , Leo Preston Vernon

Retrospective Theses and Dissertations

No abstract provided.