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Iowa State University

Soybean

Plant Biology

Graduate Theses and Dissertations

Publication Year

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Microscopic Characterization Of A Transposon-Induced Male-Sterile, Female-Sterile Mutant In Glycine Max L., Katherine A. Thilges Jan 2016

Microscopic Characterization Of A Transposon-Induced Male-Sterile, Female-Sterile Mutant In Glycine Max L., Katherine A. Thilges

Graduate Theses and Dissertations

A male-sterile, female-sterile mutant was identified in a transposon-tagging study in soybean (Glycine max L.). This mutant displayed abnormalities in both micro- and mega-sporogenesis, as well as gametogenesis. Vegetatively, the mutant showed no visible differences from the wild-type phenotype. Analyses of male meiotic chromosomes were done to better understand any issues that could occur to cause sterility. Wild-type and mutant anthers and ovules were cleared throughout their development, followed by confocal scanning laser microscopy to look for any abnormalities, and to determine the timing of abortion in both the male and female organs in the mutant. Additionally, scanning electron microscopy ...


A Type Ii Metacaspase Interacts With Rps1-K-2 In Soybean And Analysis Of The Soybean Metacaspase Gene Family, James Andrew Baskett Jan 2012

A Type Ii Metacaspase Interacts With Rps1-K-2 In Soybean And Analysis Of The Soybean Metacaspase Gene Family, James Andrew Baskett

Graduate Theses and Dissertations

Soybean suffers from the root and stem rot disease caused by the oomycete pathogen, Phytophthora sojae. A series of Rps genes confer Phytophthora resistance, and have been utilized in fighting this serious disease in soybean. Earlier we reported the cloning of the coiled coil-nucleotide binding-leucine rich repeat gene, Rps1-k-2. Here we report the identification of a type II metacaspase, Gmmc11 that interacted with the coiled-coil (CC) domain of Rps1-k-2 in vivo in yeast. The interaction was verified by conducting in vitro pull down assays in unpublished work. RNA interference of Gmmc11 in cotyledons resulted in loss of Phytophthora resistance. Gmmc11 ...