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Iowa State University

Soybean

Plant Biology

Genetics, Development and Cell Biology Publications

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Microscopic Characterization Of A Transposon-Induced Male-Sterile, Female-Sterile Mutant In Glycine Max L., Katherine A. Thilges, Mark A. Chamberlin, Marc C. Albertsen, Harry T. Horner Oct 2017

Microscopic Characterization Of A Transposon-Induced Male-Sterile, Female-Sterile Mutant In Glycine Max L., Katherine A. Thilges, Mark A. Chamberlin, Marc C. Albertsen, Harry T. Horner

Genetics, Development and Cell Biology Publications

Premise of research. A male-sterile, female-sterile mutant was discovered in a w4-m mutable line of Glycine max L. The mechanism of its sterility was not well understood. Therefore, different cytological and microscopic techniques were undertaken to better understand the process of mutant phenotype development. Molecular research indicated that mer3 was responsible for the sterility.

Methodology. Macro images were collected of whole plants, flowers, anthers, pods, and ovules. Chromosome spreads from anthers at various meiotic stages were examined. Confocal scanning laser microscopy using optical sectioning was utilized on whole anthers and ovules at various developmental stages. Whole mature anthers and ...


Multicellular Secretory Trichome Development On Soybean And Related Glycine Gynoecia, R. A. Healy, R. G. Palmer, Harry T. Horner May 2009

Multicellular Secretory Trichome Development On Soybean And Related Glycine Gynoecia, R. A. Healy, R. G. Palmer, Harry T. Horner

Genetics, Development and Cell Biology Publications

Multicellular glandular trichomes form on gynoecia of wild annual Glycine species, annual soybean cultivars, and wild perennial Glycine species. These trichomes occur from the ovary base to the style base and, in perennial species, along the style as well.Trichomes form at least 2 d before anthesis, and new trichomes develop throughout flowering and also on young seed pods. Trichome structure is similar in all taxa examined, usually five to seven linearly arranged cells. Stalk cells with callose walls become highly vacuolate, and their cytoplasms have reduced numbers of Golgi bodies and endoplasmic reticulum. During secretion, two to four distal ...


Insect-Mediated Cross-Pollination In Soybean [Glycine Max (L.) Merrill]: Ii. Phenotypic Recurrent Selection, Evelyn Ortiz-Perez, Hunt Wiley, Harry T. Horner, William H. Davis, Reid G. Palmer Jul 2008

Insect-Mediated Cross-Pollination In Soybean [Glycine Max (L.) Merrill]: Ii. Phenotypic Recurrent Selection, Evelyn Ortiz-Perez, Hunt Wiley, Harry T. Horner, William H. Davis, Reid G. Palmer

Genetics, Development and Cell Biology Publications

Recurrent selection is a method for population improvement which has been used in soybean [Glycine max (L.) Merrill] to modify traits such as grain yield, seed-protein content, seed-oil content, tolerance to iron-deficiency chlorosis, and seed size. Nuclear male-sterility with insect-mediated cross-pollination has been successfully used in recurrent selection schemes in soybean. However, little attention has been given to selection to increase the agronomic performance of male-sterile plants per se. The objective of this study was to evaluate the response of male-sterile lines segregating for male-sterile alleles ms2, ms3, ms6, ms8, and ms9 to phenotypic recurrent selection for increased seed-set after ...


Insect-Mediated Cross-Pollination In Soybean [Glycine Max (L.) Merrill] : I. Agronomic Performance, E. Ortiz-Perez, S. R. Cianzio, H. Wiley, Harry T. Horner, W. H. Davis, R. G. Palmer Jan 2007

Insect-Mediated Cross-Pollination In Soybean [Glycine Max (L.) Merrill] : I. Agronomic Performance, E. Ortiz-Perez, S. R. Cianzio, H. Wiley, Harry T. Horner, W. H. Davis, R. G. Palmer

Genetics, Development and Cell Biology Publications

In soybean, manual cross-pollination to produce large quantities of F1 hybrid seed for yield trials is difficult and time-consuming. Conversely, insect-mediated cross-pollination has been shown to produce large quantities of hybrid seed in soybean and could facilitate the identification of heterotic patterns. The objective of our study was: (1) evaluate F1hybrid soybean plants from single crosses for yield and agronomic traits over several environments and (2) compare hybrid performance of the single crosses to lines developed from three-way crosses and backcrosses. In 2003, F1 seed of single-crosses and their parent lines were evaluated in replicated experiments ...


Insect-Mediated Seed-Set Evaluation Of 21 Soybean Lines Segregating For Male Sterility At 10 Different Loci, E. Ortiz-Perez, Harry T. Horner, S. J. Hanlin, R. G. Palmer Dec 2006

Insect-Mediated Seed-Set Evaluation Of 21 Soybean Lines Segregating For Male Sterility At 10 Different Loci, E. Ortiz-Perez, Harry T. Horner, S. J. Hanlin, R. G. Palmer

Genetics, Development and Cell Biology Publications

The first requirement to establish a successful hybrid soybean program is the availability of a stable male-sterile, female-fertile system. Male sterility has been an important tool in soybean breeding programs to improve traits such as yield, seed-protein and seed-oil content, and seed size. However, improvement of seed-set per se on male-sterile plants has not been an important breeding objective. The evaluation of the out-crossing potential of the available male-sterile, female-fertile soybean lines is crucial to determine the future of hybrid soybean. The objective of this study was to evaluate seed-set among 21 soybean lines segregating for male sterility at 10 ...


Evaluation Of Insect-Mediated Seed Set Among Soybean Lines Segregating For Male Sterility At The Ms6 Locus, E. Ortiz-Perez, Harry T. Horner, S. J. Hanlin, R. G. Palmer Jun 2006

Evaluation Of Insect-Mediated Seed Set Among Soybean Lines Segregating For Male Sterility At The Ms6 Locus, E. Ortiz-Perez, Harry T. Horner, S. J. Hanlin, R. G. Palmer

Genetics, Development and Cell Biology Publications

Currently, there is no economical way to produce large quantities of F1 hybrid soybean seed in the USA. One of the fundamental requirements for hybrid seed production is the availability of a stable male-sterile, female-fertile system. However, the more challenging barrier is the efficient transfer of pollen from the male parent to the female parent. This could potentially be achieved through pollinator insects. Our observations suggested that seed set on male-sterile, female-fertile plants is a good indicator of insect attraction. The objective of this study was to evaluate seed set among male-sterile, female-fertile lines segregating for male-sterile, female-fertile ms6 ...


A Microscopic Study Of The Trichomes On Gynoecia Of Normal And Tetraploid Clark Cultivars Of Glycine Max And Seven Near Isogenic Lines, Rosaria A. Healy, Harry T. Horner, Theodore B. Bailey, Reid G. Palmer May 2005

A Microscopic Study Of The Trichomes On Gynoecia Of Normal And Tetraploid Clark Cultivars Of Glycine Max And Seven Near Isogenic Lines, Rosaria A. Healy, Harry T. Horner, Theodore B. Bailey, Reid G. Palmer

Genetics, Development and Cell Biology Publications

The surfaces of gynoecia of Glycine max cultivars—normal Clark, a tetraploid Clark, and seven isolines—display variations in at least three types of trichomes. The normal Clark soybean gynoecium has at least three and possibly four types of trichomes: a two‐ to four‐celled, elongate, thick‐walled trichome (TWT), an elongate thin‐walled unicellular trichome (UCT), a secretory multicellular trichome (MCT), and an elongate thin‐walled bicellular trichome that we have interpreted as an immature TWT. All these types are present on the gynoecium by 1 d before anthesis. After fertilization, the UCT is rare, but the other types ...