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Iowa State University

Soybean

Entomology

2002

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An Inexpensive, Accurate Method For Measuring Leaf Area And Defoliation Through Digital Image Analysis, Matthew E. O'Neal, Douglas A. Landis, Rufus Isaacs Dec 2002

An Inexpensive, Accurate Method For Measuring Leaf Area And Defoliation Through Digital Image Analysis, Matthew E. O'Neal, Douglas A. Landis, Rufus Isaacs

Matthew E. O'Neal

We report a protocol using a common desk-top scanner and public domain software for measuring existing leaf area and leaf area removed as a result of herbivory. We compared the accuracy and precision of this method to that of a standard leaf area meter. Both methods were used to measure metal disks of a known area, the area of soybean (Glycine max L.) leaves, and the area removed by simulating leaf feeding with a hole-punch. We varied the amount of injury across a low, medium, and high degree of simulated feeding. The mean area of 10 cm2 and 50 cm2 ...