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Iowa State University

Soybean

Cell and Developmental Biology

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Microscopic Characterization Of A Transposon-Induced Male-Sterile, Female-Sterile Mutant In Glycine Max L., Katherine A. Thilges, Mark A. Chamberlin, Marc C. Albertsen, Harry T. Horner Oct 2017

Microscopic Characterization Of A Transposon-Induced Male-Sterile, Female-Sterile Mutant In Glycine Max L., Katherine A. Thilges, Mark A. Chamberlin, Marc C. Albertsen, Harry T. Horner

Genetics, Development and Cell Biology Publications

Premise of research. A male-sterile, female-sterile mutant was discovered in a w4-m mutable line of Glycine max L. The mechanism of its sterility was not well understood. Therefore, different cytological and microscopic techniques were undertaken to better understand the process of mutant phenotype development. Molecular research indicated that mer3 was responsible for the sterility.

Methodology. Macro images were collected of whole plants, flowers, anthers, pods, and ovules. Chromosome spreads from anthers at various meiotic stages were examined. Confocal scanning laser microscopy using optical sectioning was utilized on whole anthers and ovules at various developmental stages. Whole mature anthers and ...


Cloning, Mapping, And Analyses Of Expression Of The Em-Like Gene Family In Soybean [Glycine Max (L). Merr.], E. S. Calvo, E. S. Wurtele, R. C. Shoemaker Jun 1997

Cloning, Mapping, And Analyses Of Expression Of The Em-Like Gene Family In Soybean [Glycine Max (L). Merr.], E. S. Calvo, E. S. Wurtele, R. C. Shoemaker

Botany Publication and Papers

The entire Em-like Group-1 late embryogenesis abundant (Lea) gene family from soybean was cloned and characterized. The five Group-1 Lea genes (Sle1-5) were divided into two classes based on sequence identity. Sle1-4 were genetically mapped to four different linkage groups. Nucleotide sequencing indicated that Sle1, Sle2, Sle3, and Sle5 encode polypeptides differing primarily by the presence of a repeated 20-amino acid motif. Sle1 and Sle5 were shown by Northern analysis to be expressed in developing embryos weeks earlier than Sle2 and Sle3. Sle4 was shown ...