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Iowa State University

Soybean

Cell and Developmental Biology

Botany Publication and Papers

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Cloning, Mapping, And Analyses Of Expression Of The Em-Like Gene Family In Soybean [Glycine Max (L). Merr.], E. S. Calvo, E. S. Wurtele, R. C. Shoemaker Jun 1997

Cloning, Mapping, And Analyses Of Expression Of The Em-Like Gene Family In Soybean [Glycine Max (L). Merr.], E. S. Calvo, E. S. Wurtele, R. C. Shoemaker

Botany Publication and Papers

The entire Em-like Group-1 late embryogenesis abundant (Lea) gene family from soybean was cloned and characterized. The five Group-1 Lea genes (Sle1-5) were divided into two classes based on sequence identity. Sle1-4 were genetically mapped to four different linkage groups. Nucleotide sequencing indicated that Sle1, Sle2, Sle3, and Sle5 encode polypeptides differing primarily by the presence of a repeated 20-amino acid motif. Sle1 and Sle5 were shown by Northern analysis to be expressed in developing embryos weeks earlier than Sle2 and Sle3. Sle4 was shown ...