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Preparation Of Pyrrol And Oxazole Compounds; Formation Of Porphyrins And C-Acyl-Α-Amino Acid Esters Therefrom, John G. Verkade, Jianseng Tang Sep 1997

Preparation Of Pyrrol And Oxazole Compounds; Formation Of Porphyrins And C-Acyl-Α-Amino Acid Esters Therefrom, John G. Verkade, Jianseng Tang

Iowa State University Patents

A process for preparation of new or previously known pyrrol derivatives is provided. The process involves reacting a nitroalkane or a nitroalkene with an isocyanoacetate in the presence of a prophosphatrane "super base" to prepare the pyrrol compounds. Through production of new pyrrol compounds, new pyrrol derivatives such as dipyrromethanes and porphyrins may be synthesized. In addition, the high yields of pyrrol compounds produced according to the method of the invention provide for more efficient production of previously known dipyrromethane and porphyrin compounds. The invention further provides a mild, high yield process for de-esterification of esterified pyrrol and dipyrromethane compounds ...


Ketodiacid Compounds That Inhibit Nematode Egg Hatching, George A. Kraus, Gregory L. Tylka Jul 1997

Ketodiacid Compounds That Inhibit Nematode Egg Hatching, George A. Kraus, Gregory L. Tylka

Iowa State University Patents

A method for inhibiting hatching of soybean cyst nematode eggs is provided comprising contacting said eggs with an effective inhibitory amount of a compound of formula (I): ##STR1## wherein R.sup.1 is H or OH, R.sup.2 is H or OH, R.sup.3 is H or (C.sub.1 -C.sub.4)alkyl, R.sup.4 is H or (C.sub.1 -C.sub.4)alkyl, R.sup.5 and R.sup.6 are individually H, (C.sub.1 -C.sub.5)alkyl, C.sub.3 -C.sub.6 (cycloalkyl)alkyl, C.sub.5 -C.sub.7 ...


Method Of Isolating An Analyte Using A Solid Phase Extraction Medium, James S. Fritz, Philip J. Dumont, Donald F. Hagen, Craig G. Markell, Luther W. Schmidt Apr 1997

Method Of Isolating An Analyte Using A Solid Phase Extraction Medium, James S. Fritz, Philip J. Dumont, Donald F. Hagen, Craig G. Markell, Luther W. Schmidt

Iowa State University Patents

A functionalized macroporous poly(styrene divinylbenzene) particle comprises at least one ionic functional group covalently bonded thereto, the functionalized particle having sorptive capability towards an analyte, said functional group being present in the range of 0.1 to 2.5 milliequivalents per gram of poly(styrene divinylbenzene). The functionalized particles can be used in a packed column or enmeshed in a nonwoven web for utility in solid phase extraction applications.


Palladium Catalyzed Annulation Of Internal Alkynes, Richard C. Larock Jan 1997

Palladium Catalyzed Annulation Of Internal Alkynes, Richard C. Larock

Iowa State University Patents

A method is provided for the regioselective, palladium catalyzed carbo- or heteroannulation of internal alkynes by aryl iodides containing o-substituted side chains to yield aromatic carbocyclic and heterocyclic ring systems.


The Use Of Fourier Reverse Transforms In Crystallographic Phase Refinement , Sharon Lynn Ringrose Jan 1997

The Use Of Fourier Reverse Transforms In Crystallographic Phase Refinement , Sharon Lynn Ringrose

Retrospective Theses and Dissertations

Often a crystallographer obtains an electron density map which shows only part of the structure. In such cases, the phasing of the trial model is poor enough that the electron density map may show peaks in some of the atomic positions, but other atomic positions are not visible. There may also be extraneous peaks present which are not due to atomic positions. A method for determination of crystal structures that have resisted solution through normal crystallographic methods has been developed. PHASER is a series of FORTRAN programs which aids in the structure solution of poorly phased electron density maps by ...


Physicochemical Properties Of The Anion-Modified Surfaces Of Silver Nanostructures , Morgan Scot Sibbald Jan 1997

Physicochemical Properties Of The Anion-Modified Surfaces Of Silver Nanostructures , Morgan Scot Sibbald

Retrospective Theses and Dissertations

Nano-sized structures of silver were prepared, characterized, and then chemically-modified with the adsorption of molecules and ions on the metal surface. Nanostructures prepared as aqueous colloids were found to have highly regular polyhedral shapes by transmission electron microscopy. Electron diffraction techniques indicated that isolated Ag structures were composed of a single crystalline phase or were multiply-twinned, both having a lattice constant of 4.05 A;Adsorption of iodide and bromide ions on the silver surface was monitored by surface-enhanced Raman spectroscopy. A characteristic halide-metal stretching vibration was observed at 112 cm-1 for I- and at 156 cm-1 for Br-. Extinction ...


Ligand Effects On The Heats Of Protonation Of Metal-Metal Bonds In Organometallic Complexes , Chip Nataro Jan 1997

Ligand Effects On The Heats Of Protonation Of Metal-Metal Bonds In Organometallic Complexes , Chip Nataro

Retrospective Theses and Dissertations

Calorimetric determination of the metal center basicity in organometallic complexes has proven to be a useful tool for understanding reactivity trends. Similar studies conducted on metal-metal bonded species to determine discrete metal-metal bond basicity could offer a simple model of reactivity for transition metal surfaces. However, few examples of dinuclear compounds exist in which protonation occurs at the metal-metal bond to form the bridging hydride. In this research, the basicities of the metal-metal bond in Ru2Cp'2(CO)3L and Mo2Cp2(CO)4L2 have been determined. Basicities have been measured by the heat evolved ([delta] HMHM) when a metal complex ...


Monitoring Protein Refolding Using Diverse Separation And Spectroscopy Techniques , Pamela Kay Jensen Jan 1997

Monitoring Protein Refolding Using Diverse Separation And Spectroscopy Techniques , Pamela Kay Jensen

Retrospective Theses and Dissertations

Rapid growth in the biotechnology industry has led to a dramatic increase in attention to the protein folding problem. Understanding of protein folding is essential to the production of biopharmaceuticals since commercial production of recombinant proteins often requires a protein refolding process to recover high yields;Capillary zone electrophoresis (CZE) equipped with laser induced fluorescence detection (LIFD) is developed as a tool for monitoring the refolding of a model protein, phage P22 tailspike endorhamnosidase. Intermediates on the folding pathway are separated in CZE and monitored by their intrinsic tryptophan fluorescence. Monitoring refolding of tailspike by traditional methods, sodium dodecyl sulfate-polyacrylamide ...


Palladium(Ii)-Catalyzed Organic Transformations Employing Oxygen Gas As The Stoichiometric Reoxidant , Karl Patrick Peterson Jan 1997

Palladium(Ii)-Catalyzed Organic Transformations Employing Oxygen Gas As The Stoichiometric Reoxidant , Karl Patrick Peterson

Retrospective Theses and Dissertations

The organic transformations promoted by palladium(II) species have historically required either stoichiometric amounts of metal or the stoichiometric use of a chemical reoxidant, such as benzoquinone or Cu(II) salts. In recent years, several reports have described the use of oxygen gas as the stoichiometric reoxidant in Pd(II) chemistry. This advance has made the chemistry of Pd(II) more attractive because of reductions in cost and waste generation. This development has also encouraged researchers to probe the scope and limitation of Pd(II) chemistry using oxygen gas as the sole reoxidant of the metal. Three such organic transformations ...


New Chemical Sources Of Energy: A Theoretical Study , Galina Chaban Jan 1997

New Chemical Sources Of Energy: A Theoretical Study , Galina Chaban

Retrospective Theses and Dissertations

The research presented in this dissertation employs methods of quantum chemistry for the search of highly energetic chemical compounds that can have applications as possible energy sources. The areas of research include: (1) improvement of orbital optimization methods for different types of wavefunctions which leads to substantial savings of computer time and memory; (2) predicting new high energy isomers for singlet and triplet states of N3F and their kinetic stability with respect to isomerisation and dissociation reactions; (3) estimation of minimum energy reaction paths for dissociation reactions of high energy isomers of N2O2 including potential energy barriers and minimum energy ...


Novel Materials And Methods For Solid-Phase Extraction And High-Performance Liquid Chromatography , Dianna Lynn Ambrose Jan 1997

Novel Materials And Methods For Solid-Phase Extraction And High-Performance Liquid Chromatography , Dianna Lynn Ambrose

Retrospective Theses and Dissertations

Solid-phase extraction (SPE) is one of the most popular sample preparation techniques for the isolation and preconcentration of organic compounds from aqueous samples. Modified porous polystyrene-divinylbenzene (PS-DVB) resins containing polar groups are superior for SPE of organic solutes from aqueous samples. These modified resins display excellent surface hydrophilicity and improved extraction efficiencies. Silicalite is a hydrophobic molecular sieve with an unusual crystal structure. Because of its unique structure, Silicalite adsorbs organic analytes from aqueous samples due to hydrophobic interactions;The ability of Silicalite and a lightly sulfonated PS-DVB resin to extract various organic test compounds from aqueous samples was compared ...


Photodissociation Dynamics Of Polyatomic Molecules , Hequan Zhao Jan 1997

Photodissociation Dynamics Of Polyatomic Molecules , Hequan Zhao

Retrospective Theses and Dissertations

In the photodissociation of acetophenone (C6H5COCH3) at 193 nm, two major primary processes, C6H5COCH3 + h[nu] → C6H5CO + CH3 (process 1) and C6H5 + COCH3 (process 2), are observed. Data analysis shows that excited C6H5CO and CH3CO thus formed further decompose, yielding secondary products C6H5 + CO and CH3 + CO, respectively. For 248 nm excitation, the C6H5CO-CH3 bond energy has been determined to be 80.2 ± 3 kcal/mol. Evidence is also found that the dissociation reaction C6H5COCH3 + hv→ C6H5CH3 + CO occurs for excitation at both 193 and 248 nm;In the photodissociation of dimethylsulfoxide [(CH3)2SO] at 193.3 nm, in addition ...


Unimolecular Dissociation Of Polyatomic Ions By Molecular Beam Photoionization Mass Spectrometry And Collision-Induced Dissociation , Yu-Ju Chen Jan 1997

Unimolecular Dissociation Of Polyatomic Ions By Molecular Beam Photoionization Mass Spectrometry And Collision-Induced Dissociation , Yu-Ju Chen

Retrospective Theses and Dissertations

The photoionization efficiency (PIE) specta for M(CO)n+ (n = 0-6) from M(CO)6, M = Cr, Mo, and W, have been measured in the photon energy range of 650-1600 A. Based on the ionization energies for M(CO)6 and appearance energies (AEs) for M(CO)n+ (n = 0-5) determined here, we have obtained estimates for the sequential bond dissociation energies (D0) for CO-M(CO)n-1+ (n = 1-6). The comparison of the first D0 values for M(CO)6+ obtained here and those for M(CO)6 provides strong support for the theoretical analysis that the importance of relativistic ...


Derivatives, Dimers, And Solutions , Simon Paul Webb Jan 1997

Derivatives, Dimers, And Solutions , Simon Paul Webb

Retrospective Theses and Dissertations

The research presented in this thesis may be divided into three areas: derivative theory, transition metal chemistry, and solvation effects;Analytic derivatives. A derivation of the closed shell frozen-core analytic gradient for second order Moller Plesset perturbation (MP2) theory is presented, as well as a derivation of an expression for the localized orbital dipole polarizability. Both methods have been implemented in the electronic structure code GAMESS and are currently in wide use;Transition metal chemistry. Ab initio electronic structure theory methods have been used to investigate the chemistry of simple titanium compounds. These include the closed shell TiH4 dimer: Ti2H8 ...


Synthesis And Characterization Of Novel Group Vi Metal (Mo, W) Nitride And Oxide Compounds , Zhihong Zhang Jan 1997

Synthesis And Characterization Of Novel Group Vi Metal (Mo, W) Nitride And Oxide Compounds , Zhihong Zhang

Retrospective Theses and Dissertations

Binary Group VI metal nitrides are characterized by thermodynamic instability towards dissociation to N2 and the N-saturated elements at high temperature. Our investigations into the preparation of tungsten nitrides have involved the synthesis of molecular precursors, and their conversion to tungsten nitrides at relatively low temperatures. Two interesting molecular precursors, (WNCl3·NCCH3] 4 and WN(N3)3· xNCCH3, have been prepared and characterized. The molecular structure of (WNCl3·NCCH3] 4 consists of a W4N4 tetrameric core with multiple and single W-N bonds arranged in an alternating fashion. Three new solid state phases, amorphous W3N5, cubic WN, and W2N2(C2N2), have ...


Separation Of Ions In Acidic Solution By Capillary Electrophoresis, Michelle Jo Thornton Jan 1997

Separation Of Ions In Acidic Solution By Capillary Electrophoresis, Michelle Jo Thornton

Retrospective Theses and Dissertations

Capillary electrophoresis (CE) is an effective method for separating ionic species according to differences in their electrophoretic mobilities. Separations of anions are usually achieved by adding a quaternary ammonium salt to the electrolyte solution to reverse the electroosmotic flow (EOF). It is now shown that excellent separations of inorganic anions such as the anionic chloro complexes of the platinum group elements (PGEs) and anionic metal oxides are obtained using an acidic aqueous electrolyte carrier. The EOF is minimized at an acidic pH which eliminates the need to reverse it. The acidic solution also helps to stabilize some analytes such as ...


Design, Synthesis, Characterization And Study Of Novel Conjugated Polymers , Wu Chen Jan 1997

Design, Synthesis, Characterization And Study Of Novel Conjugated Polymers , Wu Chen

Retrospective Theses and Dissertations

A series of conjugated polymers was designed and synthesized. The chemical structures of the polymers were characterized by 1H- and 13C-NMR, and the molecular weights were measured by GPC. Their thermal behaviors were studied by DSC and TGA. Their UV/Vis absorption, fluorescence and conductivity were also studied. The copolymers having repeating units with combinations of poly(phenylenevinylene) (PPV), poly(phenyleneethynylene) (PPE) and poly(paraphenylene) (PPP) were usually strongly luminescent. The poly(phenylenevinylene) and poly(phenyleneethynylene) copolymer-based LED device showed symmetric I-V curve, which was quite different from non-symmetric curves reported for PPV-based or PPE-based LEDs. The values of the ...


Synthetic Approaches To Natural Products , John Herman Malpert Jan 1997

Synthetic Approaches To Natural Products , John Herman Malpert

Retrospective Theses and Dissertations

The total synthesis of natural products and their analogs is a very exciting and challenging area of organic chemistry. The biological activities of these natural products make this area of chemistry popular. Our synthetic approaches towards the biologically active molecules eburnamonine, MS-444, halenaquinone and their analogs represent a valuable contribution to the field of organic chemistry;We have devised a synthetic pathway that completes four of the five rings of eburnamonine. In addition this pathway can be used to synthesize a variety of indolo(2,3-a) quinolizines, demonstrating the wide versatility of our pathway;We have also devised a synthetic ...


Interfacial Characterizations And Analytical Applications Of Chemically-Modified Surfaces , Jianhong Wang Jan 1997

Interfacial Characterizations And Analytical Applications Of Chemically-Modified Surfaces , Jianhong Wang

Retrospective Theses and Dissertations

This dissertation explores several new strategies and approaches to the surface modifications for applications in environmental monitoring, and the characterizations of interfaces at the microscopic level. The first of the four papers included in this dissertation describes the development of optical pH sensors based on the immobilization of fluoresceinamine at a base-hydrolyzed cellulose acetate film. The advantages of the sensors include a rapid response time (7 pH units), and exceptional long-term stability. The ionic strength and temperature effects, metal-ion interference, and fluorescence properties of the sensors were examined;The second paper demonstrates the in situ monitoring of the base-hydrolysis of ...


Development And Application Of Post-Capillary Affinity Detection For Capillary Electrophoresis , Jean Abler Kelly Jan 1997

Development And Application Of Post-Capillary Affinity Detection For Capillary Electrophoresis , Jean Abler Kelly

Retrospective Theses and Dissertations

Protein variants can arise from transcription errors, post-translational modifications, as well as chemical or proteolytic degradation. In this work post-capillary affinity detection for capillary electrophoresis is developed to assess protein microheterogeneity in complex sample matrices. Analyte variants are first separated by capillary zone electrophoresis due to differences in their molecular structure. Following separation, a fluorescently labeled analyte-binding protein is introduced which forms affinity complexes with protein variants. A fluorescence enhancement results from complex formation and is monitored by laser-induced fluorescence detection;Two unique affinity reaction systems are employed in post-capillary affinity detection. One system utilizes the affinity interaction of fragment ...


Exploring Aluminum-Rich Intermetallics With Experiment And Theory , Karen Jane Nordell Jan 1997

Exploring Aluminum-Rich Intermetallics With Experiment And Theory , Karen Jane Nordell

Retrospective Theses and Dissertations

A large group of ternary trielide-rich (Al, Ga, In) intermetallics forming variants of the NaZn13, ThMn12, BaCd11, BaNi2Al9, ErZn5, CaCu5, BaAl4, and [alpha]-La3Al11 structure types has been synthesized from stoichiometric combinations of the elements in an arc melting furnace or conventional high-temperature furnaces. The majority of these compounds have the general formula AMxTy, where A = Ba, Sr, La... Yb, M = Cu, Ag, Au, and T = Al, Ga, In, with x < y. Many of these phases contain complex three-dimensional networks of interconnected polyhedra, and exhibit both ordered and disordered arrangements of atoms within the structures. These ternary compounds have been characterized by both powder and single crystal X-ray diffraction. Magnetic susceptibility measurements, and energy dispersive spectroscopic measurements are reported for many of the new phases. The electronic structures of these ternary trielides have been characterized using both extended Huckel calculations, and second moment scaling calculations. The extended Huckel calculations demonstrate the tuning of these system's stoichiometries to maximize the bonding within the intermetallic frameworks, and the second moment scaling calculations were used to sort certain structure types based on narrow ranges of valence electron concentrations.


Electrochemically-Modulated Liquid Chromatography (Emlc): Column Design, Retention Processes, And Applications , En-Yi Ting Jan 1997

Electrochemically-Modulated Liquid Chromatography (Emlc): Column Design, Retention Processes, And Applications , En-Yi Ting

Retrospective Theses and Dissertations

This dissertation explores a new separation technique, electrochemically modulated liquid chromatography (EMLC), from the column design, retention processes, to the pharmaceutical applications. A literature review, general summary, and perspectives of this technique are also described. Chapter 1 presents the newly designed EMLC column. The principal modification of this design is to connect the porous stainless steel column as counter electrode as opposed to part of working electrode in the previous design. The improvement in performance from this modification results in a shorter response time to changes in applied potential (E appl) and a better control of E appl at cathodic ...


Synthesis Of Biomimetic Compounds , Steven John Vander Louw Jan 1997

Synthesis Of Biomimetic Compounds , Steven John Vander Louw

Retrospective Theses and Dissertations

The purpose of this research was to develop efficient synthesis to biologically interesting molecules. The three projects undertaken were the synthesis of natural antennas, the synthesis of soybean cyst nematode hatch inhibitors, and a study towards the total synthesis of glycinoclepin A;We have devised a synthetic pathway that completes the basic structure for an artificial light harvesting antenna. In addition this pathway can be used to synthesize a variety phthalocyanine dendrimers;We have also devised a synthetic route to potent analogs of glycinoclepin A. The analogs should prove useful in the prevention of soybean cyst nematode proliferation;Finally, we ...


Selective Spectroscopic Methods For Water Analysis , Bikas Vaidya Jan 1997

Selective Spectroscopic Methods For Water Analysis , Bikas Vaidya

Retrospective Theses and Dissertations

This dissertation explores in large part the development of a few types of spectroscopic methods in analysis of water. Methods for the determination of some of the most important properties of water like pH, metal ion content, and chemical oxygen demand are investigated in detail. The first of the five papers included in this dissertation describes the synthesis, acid-base reactivity and metal ion binding selectivity of two novel crown ether compounds, N,N'-bis(2-hydroxy-5-nitrobenzyl)-4,13-diazadibenzo-18-crown- 6 (CCE) and N,N'-bis(7-hydroxy-4-methylcoumarin-8-methylene)-4,13-diazadibenzo-18-crown-6 (FCE). Extraction constants for Ba(II), Ca(II), Cd(II), Cu(II), Hg(II ...


Photostimulated Tert-Butylations Of A,B-Unsaturated Nitriles, Cyclizations Of 1,6-Dienes And Olefinic Nitriles, And Homolytic Aromatic Alkylations , Ping Chen Jan 1997

Photostimulated Tert-Butylations Of A,B-Unsaturated Nitriles, Cyclizations Of 1,6-Dienes And Olefinic Nitriles, And Homolytic Aromatic Alkylations , Ping Chen

Retrospective Theses and Dissertations

[alpha],[beta]-Unsaturated nitriles undergo radical chain addition reactions with tert-butylmercury halides in the presence of iodide ions. The mercury adduct products are formed in high yields and can be converted to the reductive alkylation products with NH4+ or by NaBH4. The addition reaction involves the attack of the adduct radical upon the ate complex, t-BuHgI2-. Proton donors also promote the reactions by protonating the adduct radicals to form keteniminyl radicals cations which accept an electron from t-BuHgI2- to produce the alkylation products. Acrylonitrile, crotononitrile and fumaronitrile give nearly quantitative yields of the products in DMSO via photolysis or even ...


Improved Resins And Novel Materials And Methods For Solid Phase Extraction And High Performance Liquid Chromatography , Ronald Carl Freeze Jan 1997

Improved Resins And Novel Materials And Methods For Solid Phase Extraction And High Performance Liquid Chromatography , Ronald Carl Freeze

Retrospective Theses and Dissertations

Solid-phase extraction (SPE) has grown to be one of the most widely used methods for isolation and preconcentration of a vast range of compounds from aqueous solutions. By modifying polymeric SPE resins with chelating functional groups, the selective uptake of metals was accomplished. By reducing the size of the resin beads and optimizing reaction conditions, resins were produced which had excellent capacities both in the packed-column and the suspension mode. By reducing bead sizes to 1 [mu]m, direct injection of the beads into an inductively coupled plasma (ICP) torch was possible. The resin, along with adsorbed metals, was vaporized ...


Synthesis, Characterization And Reactions Of Complexes Containing New Ligands With Carbon-Phosphorus Multiple Bonds , Wayde Vincent Konze Jan 1997

Synthesis, Characterization And Reactions Of Complexes Containing New Ligands With Carbon-Phosphorus Multiple Bonds , Wayde Vincent Konze

Retrospective Theses and Dissertations

The coordination chemistry of complexes containing carbon-phosphorus multiple bonds has recently evolved at a very rapid pace. Recently, two different types of coordinated isocyaphide (C≡PR) ligands have been prepared; complex 1 exhibits a semibridging isocyaphide ligand, while complex 2 contains a symmetrically-bridged isocyaphide ligand. These ligands are phosphorus analogs of isocyanide ligands (C≡NR) and were prepared in the coordination spheres of transition metal complexes, as free C≡PR ligands are unknown. In attempts to prepare further examples of coordinated isocyaphide ligands to compare their bonding properties with those of isocyanides, we have succeeded in preparing some very interesting ...


Equilibrium Binding Studies Of Mono, Di, And Triisocyanide Ligands On Au Powder Surfaces , Allyn Ontko Jan 1997

Equilibrium Binding Studies Of Mono, Di, And Triisocyanide Ligands On Au Powder Surfaces , Allyn Ontko

Retrospective Theses and Dissertations

Our group has previously shown that isocyanides are readily adsorbed from solutions to Au powder and bind to the Au surface in an end-on fashion through the terminal carbon. Later work demonstrated that the equilibrium constants for the reversible adsorption (eq 1) of electronically inequivalent isocyanides could be obtained using the Langmuir isotherm technique. This Au(s) + RNC ⇌ Au(s)/(RNC) (1)dissertation describes two projects completed which complement the initial findings of this group;Initially, several alkylisocyanides (RNC = n-C4H9NC, n-C6H13NC, n-C8H17NC, n-C12H25NC, and n-C18H37NC) were synthesized to examine the effect of tail length on Au powder adsorption. It was ...


Photoinduced Metalloprotein Electron-Transfer In Solid And In Solution, Chengyu Shen Jan 1997

Photoinduced Metalloprotein Electron-Transfer In Solid And In Solution, Chengyu Shen

Retrospective Theses and Dissertations

This dissertation consists of two parts: the electron-transfer reaction in solution and in sol-gel glasses. In the first part, circular dichroism and resonance Raman spectra were used to characterize the solution structure of a very important protein, zinc-substituted cytochrome c, (designated Zncyt). The solution reactions of Zncyt with small redox quenchers and large metalloprotein (plastocyanin) were also studied. In the former case, the first example of reductive quenching of triplet Zncytc were proposed; in the later case, we studied the effects of plastocyanin mutation and the effects of solution viscosity on the rates of photoinduced electron transfer. Activation parameters were ...


Direct Methods For Dynamic Monitoring Of Secretions From Single Cells By Capillary Electrophoresis And Microscopy With Laser-Induced Native Fluorescence Detection , Wei Tong Jan 1997

Direct Methods For Dynamic Monitoring Of Secretions From Single Cells By Capillary Electrophoresis And Microscopy With Laser-Induced Native Fluorescence Detection , Wei Tong

Retrospective Theses and Dissertations

Microscale separation and detection methods for real-time monitoring of dynamic cellular processes (e.g., secretion) by capillary electrophoresis (CE) and microscopic imaging were developed. Ultraviolet laser-induced native fluorescence (LINF) provides simple, sensitive and direct detection of neurotransmitters and proteins without any derivatization;An on-column CE-LINF protocol for quantification of the release from single cell was demonstrated. Quantitative measurements of both the amount of insulin released from and the amount remaining in the cell ([beta]PTC3) were achieved simultaneously;Secretion of catecholamines (norepinephrine (NE) and epinephrine (E)) from individual bovine adrenal chromaffin cells was determined using the on-column CE-LINF. No apparent ...