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Allozyme Variation In Domesticated Annual Sunflower And Its Wild Relatives, Richard Clark Cronn, M. Brothers, Kay Ellen Klier, P. K. Bretting, Jonathan F. Wendel Sep 1997

Allozyme Variation In Domesticated Annual Sunflower And Its Wild Relatives, Richard Clark Cronn, M. Brothers, Kay Ellen Klier, P. K. Bretting, Jonathan F. Wendel

Botany Publication and Papers

The annual sunflower (Helianthus annuus L.) is a morphologically and genetically variable species composed of wild, weedy, and domesticated forms that are used for ornament, oilseed, and edible seeds. In this study, we evaluated genetic variation in 146 germplasm accessions of wild and domesticated sunflowers using allozyme analysis. Results from this survey showed that wild sunflower exhibits geographically structured genetic variation, as samples from the Great Plains region of the central United States were genetically divergent from accessions from California and the southwestern United States. Sunflower populations from the Great Plains harbored greater allelic diversity than did wild sunflower from ...


Quantitative Determination Of Calcium Oxalate And Oxalate In Developing Seeds Of Soybean (Leguminosae), H. Ilarslan, R. G. Palmer, J. Imsande, Harry T. Horner Aug 1997

Quantitative Determination Of Calcium Oxalate And Oxalate In Developing Seeds Of Soybean (Leguminosae), H. Ilarslan, R. G. Palmer, J. Imsande, Harry T. Horner

Botany Publication and Papers

Developing soybean seeds accumulate very large amounts of both soluble oxalate and insoluble crystalline calcium (Ca) oxalate. Use of two methods of detection for the determination of total, soluble, and insoluble oxalate revealed that at +16 d postfertilization, the seeds were 24% dry mass of oxalate, and three-fourths of this oxalate (18%) was bound Ca oxalate. During later seed development, the dry mass of oxalate decreased. Crystals were isolated from the seeds, and X-ray diffraction and polarizing microscopy identified them as Ca oxalate monohydrate. These crystals were a mixture of kinked and straight prismatics. Even though certain plant tissues are ...


Cloning, Mapping, And Analyses Of Expression Of The Em-Like Gene Family In Soybean [Glycine Max (L). Merr.], E. S. Calvo, E. S. Wurtele, R. C. Shoemaker Jun 1997

Cloning, Mapping, And Analyses Of Expression Of The Em-Like Gene Family In Soybean [Glycine Max (L). Merr.], E. S. Calvo, E. S. Wurtele, R. C. Shoemaker

Botany Publication and Papers

The entire Em-like Group-1 late embryogenesis abundant (Lea) gene family from soybean was cloned and characterized. The five Group-1 Lea genes (Sle1-5) were divided into two classes based on sequence identity. Sle1-4 were genetically mapped to four different linkage groups. Nucleotide sequencing indicated that Sle1, Sle2, Sle3, and Sle5 encode polypeptides differing primarily by the presence of a repeated 20-amino acid motif. Sle1 and Sle5 were shown by Northern analysis to be expressed in developing embryos weeks earlier than Sle2 and Sle3. Sle4 was shown ...


Development And Implementation Of An Integrated Science Course For Elementary Education Majors, Mickey E. Gunter, Steven D. Gammon, Robert J. Kearney, Brenda E. Waller, David J. Oliver Feb 1997

Development And Implementation Of An Integrated Science Course For Elementary Education Majors, Mickey E. Gunter, Steven D. Gammon, Robert J. Kearney, Brenda E. Waller, David J. Oliver

Botany Publication and Papers

In an effort to improve teacher preparation, a group of faculty from four separate disciplines—chemistry, geology, biology, and physics—worked together to develop an integrated science course tailored for elementary education majors. This four-credit course offered through the university's Interdisciplinary Studies Division fulfills part of the elementary education science requirements. The participating faculty receive released time from their individual departments for their involvement in the development and implementation of the course. The course is offered during both the fall and spring semesters. Currently, the university is seeking to develop a university-wide integrated science course using the elementary education ...


Genetics And Cytology Of A New Genic Male-Sterile Soybean [Glycine Max (L.) Merr.], Wei Jin, Harry T. Horner, Reid G. Palmer Feb 1997

Genetics And Cytology Of A New Genic Male-Sterile Soybean [Glycine Max (L.) Merr.], Wei Jin, Harry T. Horner, Reid G. Palmer

Botany Publication and Papers

Genetic and cytological studies were conducted with a new male-sterile, female-fertile soybean [Glycine max (L.) Merr.] mutant. This mutant was completely male sterile and was inherited as a single-recessive gene. No differences in female or male gamete transmission of the recessive allele were observed between reciprocal cross-pollinations in the F1 or F2 generations. This mutant was not allelic to any previously identified soybean genic male-sterile mutants: ms1, ms2, ms3, ms4, ms5, or ms6. No linkage was detected between sterility and flower color (W1 locus), or between sterility and pubescence color (T1 locus). Light microscopic and cytological observations of microsporogenesis in ...


Structure Of The Cac1 Gene And In Situ Characterization Of Its Expression (The Arabidopsis Thaliana Gene Coding For The Biotin-Containing Subunit Of The Plastidic Acetyl-Coenzyme A Carboxylase), Jinshan Ke, Joong-Kook Choi, Marianne Smith, Harry T. Horner, Basil J. Nikolau, Eve Syrkin Wurtele Feb 1997

Structure Of The Cac1 Gene And In Situ Characterization Of Its Expression (The Arabidopsis Thaliana Gene Coding For The Biotin-Containing Subunit Of The Plastidic Acetyl-Coenzyme A Carboxylase), Jinshan Ke, Joong-Kook Choi, Marianne Smith, Harry T. Horner, Basil J. Nikolau, Eve Syrkin Wurtele

Botany Publication and Papers

The CAC1 gene of Arabidopsis thaliana that codes for the biotin carboxyl-carrier subunit of the heteromeric acetyl-coenzyme A carboxylase was isolated and sequenced. CAC1 is a single-copy gene interrupted by six introns. Subcellular immunogold labeling indicates that the biotin carboxyl-carrier subunit is localized in the stroma of the plastids and chloroplasts. The CAC1 mRNA accumulates throughout developing embryos and ovules of siliques at a time of rapid growth and oil accumulation (7 d after flowering), but is present at much lower levels in wall cells and central septal cells of the silique. Immunolocalization studies show that the pattern of accumulation ...


Cytology And Genetics Of A Tissue Culture-Derived Soybean Genic Male-Sterile, Female-Sterile, H. Ilarslan, H. T. Skorupska, Harry T. Horner, R. G. Palmer Jan 1997

Cytology And Genetics Of A Tissue Culture-Derived Soybean Genic Male-Sterile, Female-Sterile, H. Ilarslan, H. T. Skorupska, Harry T. Horner, R. G. Palmer

Botany Publication and Papers

A completely male-sterile, female-sterile mutant was derived from tissue culture of cv. Calland. The primary goal was to identify its inheritance, allelism, and cytology. Calland sterile (TC) was nonallelic with st2, st3, st4, and st5 female-sterile and malesterile mutants. In all crosses, except st5, single-gene recessive inheritance was documented. Critical ratios, 15 fertile: 1 sterile (for two loci) and 45 fertile: 19 sterile (for three loci), were observed in the F2 families from the cross of heterozygous Calland TC andSt5st5. F3 segregation data confirmed the duplicate factor inheritance of ...