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Iowa State University

1997

Animal Science Publications

Poultry or Avian Science

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Functional Characterization Of A Chicken Major Histocompatibility Complex Class Ii B Gene Promoter, Yunfei Chen, Hyun S. Lillehoj, Chung-Hsin Hsu, Susan L. Carpenter, Susan J. Lamont Jan 1997

Functional Characterization Of A Chicken Major Histocompatibility Complex Class Ii B Gene Promoter, Yunfei Chen, Hyun S. Lillehoj, Chung-Hsin Hsu, Susan L. Carpenter, Susan J. Lamont

Animal Science Publications

A 0.7 kilobase (kb) DNA fragment from the 5' flanking region of a chicken major histocompatibility complex (MHC) class II B gene was cloned into chloramphenicol acetyltransferase (CAT) reporter vectors and was transfected into a chicken macrophage cell line that expresses a low level of MHC class II antigens. Positive orientation-dependent promoter activity of the chicken DNA was evident in a reporter construct containing an SV40 enhancer. Deletion analysis of this 0.7 kb DNA fragment revealed a short fragment in the 3' end that was crucial for the promoter function and negative regulatory elements (NRE) located further upstream ...