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A Nuclear Juvenile Hormone-Binding Protein From Larvae Of Manduca Sexta: A Putative Receptor For The Metamorphic Action Of Juvenile Hormone, Subba R. Palli, Kazushige Touhara, Jean-Philippe Charles, Bryony C. Bonning, Jeffrey K. Atkinson, Stephen C. Trowell, Kiyoshi Hiruma, Walter G. Goodman, Themis Kyriakides, Glenn D. Prestwich, Bruce D. Hammock, Lynn M. Ridiford
A Nuclear Juvenile Hormone-Binding Protein From Larvae Of Manduca Sexta: A Putative Receptor For The Metamorphic Action Of Juvenile Hormone, Subba R. Palli, Kazushige Touhara, Jean-Philippe Charles, Bryony C. Bonning, Jeffrey K. Atkinson, Stephen C. Trowell, Kiyoshi Hiruma, Walter G. Goodman, Themis Kyriakides, Glenn D. Prestwich, Bruce D. Hammock, Lynn M. Ridiford
Bryony C. Bonning
A 29-kDa nuclear juvenile hormone (JH)-binding protein from the epidermis of Manduca sexta larvae was purified by using the photoaffinity analog for JH II ([3H]epoxyhomofarnesyldiazoacetate) and partially sequenced. A 1.1-kb cDNA was isolated by using degenerate oligonucleotide primers for PCR based on these sequences. The cDNA encoded a 262-amino acid protein that showed no similarity with other known proteins, except for short stretches of the interphotoreceptor retinoid-binding protein, rhodopsin, and human nuclear protein p68. Recombinant baculovirus containing this cDNA made a 29-kDa protein that was covalently modified by [3H]epoxyhomofarnesyldiazoacetate and specifically bound the natural enantiomer of JH I (Kd = …
Insect Control By Use Of Recombinant Baculoviruses Expressing Juvenile Hormone Esterase, Bryony C. Bonning, Bruce D. Hammock
Insect Control By Use Of Recombinant Baculoviruses Expressing Juvenile Hormone Esterase, Bryony C. Bonning, Bruce D. Hammock
Bryony C. Bonning
Anti-juvenile hormone activity, and the resulting cessation of feeding, has long been considered by the agricultural chemical industry as a means for insect control. Juvenile hormone esterase (JHE) contributes to the decline in JH titer at certain times during larval development. This chapter reviews the efforts made to develop a fast acting recombinant baculovirus insecticide by creating a virus which will express artificially high levels of JHE in the insect at inappropriate times. The findings are discussed in the light of our current understanding of the regulation of larval development and the role of JHE. Prospects for the newly developed, …