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Prediction Of Chicken Embryo Lethality With The Avian Escherichia Coli Traits Complement Resistance, Colicin V Production, And Presence Of The Increased Serum Survival Gene Cluster (Iss), Penelope S. Gibbs, John J. Maurer, Lisa K. Nolan, Richard E. Wooley
Prediction Of Chicken Embryo Lethality With The Avian Escherichia Coli Traits Complement Resistance, Colicin V Production, And Presence Of The Increased Serum Survival Gene Cluster (Iss), Penelope S. Gibbs, John J. Maurer, Lisa K. Nolan, Richard E. Wooley
Lisa K. Nolan
Differentiating between virulent and avirulent avian Escherichia coli isolates continues to be a problem for poultry diagnostic laboratories and the study of colibacillosis in poultry. The ability of a laboratory to conduct one simple test that correlates with virulence would simplify studies in these areas; however, previous studies have not enabled researchers to establish such a test. In this study, the occurrence of certain phenotypic and genotypic traits purported to contribute to avian E. coli virulence in 20 avian E. coli isolates was correlated with the results of embryo challenge studies. This analysis was undertaken in an effort to determine …
Analysis Of Plasmids Cloned From A Virulent Avian Escherichia Coli And Transformed Into Escherichia Coli Dh5Α, Richard E. Wooley, Penelope S. Gibbs, Harry W. Dickerson, John Brown, Lisa K. Nolan
Analysis Of Plasmids Cloned From A Virulent Avian Escherichia Coli And Transformed Into Escherichia Coli Dh5Α, Richard E. Wooley, Penelope S. Gibbs, Harry W. Dickerson, John Brown, Lisa K. Nolan
Lisa K. Nolan
Three of four plasmids from a virulent wild-type avian Escherichia coli were cloned or transformed into an avirulent laboratory recipient E. coli DH5a and tested for the ability to confer a virulence phenotype. The three plasmids transformed into E. coli DH5a were 5, 6, and 56 kb. A fourth plasmid of 64 kb was not successfully transformed. Parameters used to measure virulence included presence of type 1 pili and a smooth lipopolysaccharide (LPS) layer, motility, production of Colicin V, resistance to host complement, and embryo lethality. The 5-kb plasmid encoded for ampicillin resistance, whereas the 6-kb plasmid encoded for tetracycline …