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Monoclonal Antibodies To Avian Escherichia Coli Iss, Steven L. Foley, Shelley M. Horne, Catherine W. Giddings, Thomas R. Gustad, Erick D. Handegard, Michael Robinson, Lisa K. Nolan
Monoclonal Antibodies To Avian Escherichia Coli Iss, Steven L. Foley, Shelley M. Horne, Catherine W. Giddings, Thomas R. Gustad, Erick D. Handegard, Michael Robinson, Lisa K. Nolan
Lisa K. Nolan
Escherichia coli infections are a major problem for the poultry industry in the United States. Yet, the virulence mechanisms operative in avian E. coli are poorly understood. In the present studies, monoclonal antibodies (MAbs) have been generated that may facilitate study of the pathogenesis of avian colibacillosis. These MAbs are directed against the Iss protein because results from our laboratory have shown that the possession of iss DNA sequences is strongly correlated with the E. coli implicated in avian colibacillosis. As part of an overall effort to explore the role of iss/Iss in colibacillosis pathogenesis, Iss protein has been purified, …
Complement Resistance, As Determined By Viable Count And Flow Cytometric Methods, And Its Association With The Presence Of Iss And The Virulence Of Avian Escherichia Coli, Lisa K. Nolan, Catherine W. Giddings, Shelley M. Horne, Curt Doetkott, Penelope S. Gibbs, Richard E. Wooley, Steven L. Foley
Complement Resistance, As Determined By Viable Count And Flow Cytometric Methods, And Its Association With The Presence Of Iss And The Virulence Of Avian Escherichia Coli, Lisa K. Nolan, Catherine W. Giddings, Shelley M. Horne, Curt Doetkott, Penelope S. Gibbs, Richard E. Wooley, Steven L. Foley
Lisa K. Nolan
Previous work in our labs has shown that avian Escherichia coli virulence is correlated with resistance to complement. Also, our studies have revealed that the presence of the increased serum survival gene (iss), known to contribute to the complement resistance and virulence of mammalian E. coli, may predict the virulent nature of an avian E. coli isolate. This relationship warrants further research, but further clarification of the relationship among virulence, complement resistance, and iss sequences requires use of complement susceptibility assays. Such assays, unfortunately, are labor-intensive, expensive, and difficult to perform. In the present study, the results of two complement …
Location Of Increased Serum Survival Gene And Selected Virulence Traits On A Conjugative R Plasmid In An Avian Escherichia Coli Isolate, Timothy J. Johnson, Catherine W. Giddings, Shelley M. Horne, Penelope S. Gibbs, Richard E. Wooley, Jerod Skyberg, Pam Olah, Ronda Kercher, Julie S. Sherwood, Steven L. Foley, Lisa K. Nolan
Location Of Increased Serum Survival Gene And Selected Virulence Traits On A Conjugative R Plasmid In An Avian Escherichia Coli Isolate, Timothy J. Johnson, Catherine W. Giddings, Shelley M. Horne, Penelope S. Gibbs, Richard E. Wooley, Jerod Skyberg, Pam Olah, Ronda Kercher, Julie S. Sherwood, Steven L. Foley, Lisa K. Nolan
Lisa K. Nolan
Avian colibacillosis is a costly disease for the poultry industry. The mechanisms of virulence employed by the etiologic agent of this disease remain ill defined. However, accumulated evidence suggests that complement resistance and the presence of the increased serum survival gene (iss) in an avian Escherichia coli isolate may be indicative of its ability to cause disease. This association of iss with the E. coli implicated in avian disease may mean that iss and/or, perhaps, the genes associated with it are important contributors to avian E. coli virulence. For this reason, we have begun a search for iss's location in …
Virulence Factors Of Escherichia Coli From Cellulitis Or Colisepticemia Lesions In Chickens, J. S. Jeffrey, Lisa K. Nolan, K. H. Tonooka, S. Wolfe, C. W. Giddings, S. M. Horne, S. L. Foley, A. M. Lynne, J. O. Ebert, L. M. Elijah, G. Bjorklund, S. J. Pfaff-Mcdonough, R. S. Singer, C. Doetkott
Virulence Factors Of Escherichia Coli From Cellulitis Or Colisepticemia Lesions In Chickens, J. S. Jeffrey, Lisa K. Nolan, K. H. Tonooka, S. Wolfe, C. W. Giddings, S. M. Horne, S. L. Foley, A. M. Lynne, J. O. Ebert, L. M. Elijah, G. Bjorklund, S. J. Pfaff-Mcdonough, R. S. Singer, C. Doetkott
Lisa K. Nolan
This study was designed to compare virulence factors of cellulitis-derived Escherichia coli to colisepticemic E. coli in order to clarify whether E. coli associated with cellulitis comprise a unique subset of pathogenic E. coli. Isolates were tested for serotype, capsule, aerobactin production, colicin production, the presence of the iss gene, and serum resistance. Untypable isolates made up the greatest percentage of each group. Serotypes O2 and O78 were the most commonly identified among both groups of isolates. No statistical differences in the distribution of aerobactin or colicin production, capsule, or iss gene were observed between groups. Cluster analysis showed that …
Complement Resistance-Related Traits Among Escherichia Coli Isolates From Apparently Healthy Birds And Birds With Colibacillosis, Samantha J. Pfaff-Mcdonough, Shelley M. Horne, Catherine W. Giddings, Jessica O. Ebert, Curt Doetkott, M. Herbert Smith, Lisa K. Nolan
Complement Resistance-Related Traits Among Escherichia Coli Isolates From Apparently Healthy Birds And Birds With Colibacillosis, Samantha J. Pfaff-Mcdonough, Shelley M. Horne, Catherine W. Giddings, Jessica O. Ebert, Curt Doetkott, M. Herbert Smith, Lisa K. Nolan
Lisa K. Nolan
In this study, 294 Escherichia coli isolates from birds with colibacillosis were collected from disease outbreaks throughout the United States and were compared with 75 fecal E. coli isolates of apparently healthy chickens by their possession of several purported virulence genes, resistance to rough-lipopolysaccharide-specific bacteriophages (rLPSr), and elaboration of capsule. Traits were selected for study on the basis of their association with complement resistance. The genes targeted in this study included those encoding colicin V (cvaC) and the outer membrane proteins TraT (traT), OmpA (ompA), and Iss (iss). No significant differences were found between the two groups of isolates in …
Further Characterization Of A Complement-Sensitive Mutant Of A Virulent Avian Escherichia Coli Isolate, Theodore J. Kottom, Lisa K. Nolan, Michael Robinson, John Brown, Tom Gustad, Shelley M. Horne, Catherine W. Giddings
Further Characterization Of A Complement-Sensitive Mutant Of A Virulent Avian Escherichia Coli Isolate, Theodore J. Kottom, Lisa K. Nolan, Michael Robinson, John Brown, Tom Gustad, Shelley M. Horne, Catherine W. Giddings
Lisa K. Nolan
An attempt was made to characterize the mechanism of complement resistance operating in a virulent avian Escherichia coli isolate. Using flow cytometry to detect antibody to C3, we found that there was significantly more antibody bound to a complement-sensitive mutant of this wild type than to the parent organism, suggesting that more C3 subunits were bound to the wild type. Neither the wild type nor the mutant degraded C3. Further, the mutant was phagocytosed to a significantly greater degree than the wild type by cultured phagocytes in the presence of C5-deficient serum. These data suggest that the wild type is …