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Further Characterization Of A Complement-Sensitive Mutant Of A Virulent Avian Escherichia Coli Isolate, Theodore J. Kottom, Lisa K. Nolan, Michael Robinson, John Brown, Tom Gustad, Shelley M. Horne, Catherine W. Giddings
Further Characterization Of A Complement-Sensitive Mutant Of A Virulent Avian Escherichia Coli Isolate, Theodore J. Kottom, Lisa K. Nolan, Michael Robinson, John Brown, Tom Gustad, Shelley M. Horne, Catherine W. Giddings
Lisa K. Nolan
An attempt was made to characterize the mechanism of complement resistance operating in a virulent avian Escherichia coli isolate. Using flow cytometry to detect antibody to C3, we found that there was significantly more antibody bound to a complement-sensitive mutant of this wild type than to the parent organism, suggesting that more C3 subunits were bound to the wild type. Neither the wild type nor the mutant degraded C3. Further, the mutant was phagocytosed to a significantly greater degree than the wild type by cultured phagocytes in the presence of C5-deficient serum. These data suggest that the wild type is …