Open Access. Powered by Scholars. Published by Universities.®
Articles 1 - 3 of 3
Full-Text Articles in Entire DC Network
Cloning And Sequencing Of The Iss Gene From A Virulent Avian Escherichia Coli, Shelley M. Horne, Samantha J. Pfaff-Mcdonough, Catherine W. Giddings, Lisa K. Nolan
Cloning And Sequencing Of The Iss Gene From A Virulent Avian Escherichia Coli, Shelley M. Horne, Samantha J. Pfaff-Mcdonough, Catherine W. Giddings, Lisa K. Nolan
Lisa K. Nolan
Control of colibacillosis is important to the poultry industry. We have found that the presence of a gene for increased serum survival, iss, is strongly correlated with Escherichia coli isolated from birds with colibacillosis. Therefore, the iss gene and its protein product, Iss, are potential targets for detection and control of avian colibacillosis. The iss gene was amplified from a virulent avian E. coli isolate and sequenced. The sequences of the gene and the predicted protein product were compared with those of iss from a human E. coli isolate and lambda bor. The iss gene from the avian E. coli …
Iss From A Virulent Avian Escherichia Coli, Steven L. Foley, Shelley M. Horne, Catherine W. Giddings, Michael Robinson, Lisa K. Nolan
Iss From A Virulent Avian Escherichia Coli, Steven L. Foley, Shelley M. Horne, Catherine W. Giddings, Michael Robinson, Lisa K. Nolan
Lisa K. Nolan
No single characteristic of virulent avian Escherichia coli has been identified that can be exploited in colibacillosis detection protocols. Research in our lab suggests a strong association between the presence of an iss DNA sequence with an isolate's disease-causing ability. The study presented here focuses on the techniques used in the expression, purification, and characterization of avian E. coli Iss protein. In brief, iss was cloned into an expression vector, the construct was transformed into a protease-deficient E. coli, and expression was induced. The protein was expressed as a glutathione-S-transferase (GST) fusion and purified by affinity chromatography. The GST portion …
Complement Resistance-Related Traits Among Escherichia Coli Isolates From Apparently Healthy Birds And Birds With Colibacillosis, Samantha J. Pfaff-Mcdonough, Shelley M. Horne, Catherine W. Giddings, Jessica O. Ebert, Curt Doetkott, M. Herbert Smith, Lisa K. Nolan
Complement Resistance-Related Traits Among Escherichia Coli Isolates From Apparently Healthy Birds And Birds With Colibacillosis, Samantha J. Pfaff-Mcdonough, Shelley M. Horne, Catherine W. Giddings, Jessica O. Ebert, Curt Doetkott, M. Herbert Smith, Lisa K. Nolan
Lisa K. Nolan
In this study, 294 Escherichia coli isolates from birds with colibacillosis were collected from disease outbreaks throughout the United States and were compared with 75 fecal E. coli isolates of apparently healthy chickens by their possession of several purported virulence genes, resistance to rough-lipopolysaccharide-specific bacteriophages (rLPSr), and elaboration of capsule. Traits were selected for study on the basis of their association with complement resistance. The genes targeted in this study included those encoding colicin V (cvaC) and the outer membrane proteins TraT (traT), OmpA (ompA), and Iss (iss). No significant differences were found between the two groups of isolates in …