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13c-Metabolic Flux Analysis Of Soybean Somatic Embryos For Identification Of Metabolic Control Points In Developing Seed, Quyen Xuan Truong Dec 2012

13c-Metabolic Flux Analysis Of Soybean Somatic Embryos For Identification Of Metabolic Control Points In Developing Seed, Quyen Xuan Truong

Graduate Theses and Dissertations

Of the world's major agronomic food crops soybeans rank highest in protein content (~40% Dwt) while also containing significant quantities of oil (~20% Dwt). Based on these unique characteristics soy has become a mainstay in world agriculture, providing a protein source for livestock and human nutrition (68% of global vegetable protein meal consumption in 2011) as well as a primary source of vegetable oil (28% of global vegetable oil consumption in 2011; http://www.soystats.com/2012). The value of soy is therefore found both in its oil and protein content and increasing the content of both is therefore ...


Variability In Soybean Agronomic Performance Traits In Response To 41 C Heat And High Relative Humidity Seed Stress, Ruth Ann Rolling Jan 2012

Variability In Soybean Agronomic Performance Traits In Response To 41 C Heat And High Relative Humidity Seed Stress, Ruth Ann Rolling

Graduate Theses and Dissertations

Incorporation of genetic diversity into soybean [Glycine max (L.) Merr.] cultivars can be used to increase yield and plant adaptation to stresses. Genetic variation has been found in commercial soybean cultivars, but using a seed stress to generate intra-cultivar variation has not been studied. The first objective of this study was to use seed stress in an attempt to generate variation in soybean agronomic traits of field stand, plant maturity, plant height, seed size, seed yield, seed protein content, seed oil content, seed standard germination and seed vigor. The second objective was to evaluate if changes in variation were heritable ...


Agronomic And Seed Traits Of Soybean Lines With Genes For Aphid Resistance, Inheritance And Mapping Of Cowpea Mild Mottle Virus-Like Resistance In Soybean, And Fatty Ester Composition Of Low-Phytate, Low-Saturate Soybean Lines, Ryan Christopher Brace Jan 2012

Agronomic And Seed Traits Of Soybean Lines With Genes For Aphid Resistance, Inheritance And Mapping Of Cowpea Mild Mottle Virus-Like Resistance In Soybean, And Fatty Ester Composition Of Low-Phytate, Low-Saturate Soybean Lines, Ryan Christopher Brace

Graduate Theses and Dissertations

Evaluation of aphid resistant backcross (BC) lines containing the Rag1 or Rag2 alleles alone, or with the two alleles combined, indicated that the mean yields of resistant BC lines were less than the mean of the two entries of their susceptible recurrent parent in each of the three experiments. This reduction in mean yield was significant for BC lines containing Rag1 and its combination with Rag2, but not for BC lines with only Rag2. Among the BC lines in each experiment, only a small percentage were significantly different from the mean yield of their susceptible recurrent parent. Significant differences in ...


Marker-Assisted Selection For Elevated Concentrations Of The Α′ Subunit Of Β-, Sheilah Emilie Oltmans-Deardorff Jan 2012

Marker-Assisted Selection For Elevated Concentrations Of The Α′ Subunit Of Β-, Sheilah Emilie Oltmans-Deardorff

Graduate Theses and Dissertations

Soybean [Glycine max (L.) Merr.] contains two major storage proteins, glycinin and β-conglycinin (BC). Glycinin accounts for approximately 40% and BC approximately 25% of total soybean seed protein (Nielsen et al., 1989). Glycinin is controlled by five genes coding for the acidic (A) and basic (B) subunits: Gy1, Gy2, Gy3, Gy4, and Gy5 (Cho et al., 1989; Nielsen et al., 1989). BC consists of gene families that encode the three major subunits α, α′, and β. The relative concentrations of the BC subunits are about 45% α, 35% α′, and 20% β (Maruyama et al., 1999). Soybean cultivars were discovered ...


Agronomic, Seed Traits And Oleate Stability Of Soybean Lines Containing The High-Oleate Transgene Dp-305423-1 And The Molecular Characterization Of The Genomic Region Flanking The High-Oleate Transgene, Jordan Dustin Spear Jan 2012

Agronomic, Seed Traits And Oleate Stability Of Soybean Lines Containing The High-Oleate Transgene Dp-305423-1 And The Molecular Characterization Of The Genomic Region Flanking The High-Oleate Transgene, Jordan Dustin Spear

Graduate Theses and Dissertations

To improve the oxidative stability of soybean [Glycine max (L.) Merr.] oil, it is desirable to increase the percentage of the monounsaturated fatty ester oleate. By altering the endogenous FAD2-1gene in soybean, oleate concentrations of greater than 750 g kg-1 can be obtained. From a commercial perspective, it is important to understand the influence of this genetic alteration on agronomic and seed traits, as well as the stability of the increased oleate concentration across a range of environments.

Objective one of my research was to evaluate the influence of the DP-305423-1 high-oleate (HO) transgene on agronomic and seed ...


The Genetics Of Fertility In Soybean, Reid G. Palmer, Devinder Sandhu, Harry T. Horner, Ram J. Singh Jan 2012

The Genetics Of Fertility In Soybean, Reid G. Palmer, Devinder Sandhu, Harry T. Horner, Ram J. Singh

Genetics, Development and Cell Biology Presentations, Posters and Proceedings

Male and female reproductive structures play an important role in seed development in plants. Abnormalities in male or female reproductive structures can lead to sterility. In soybean, Glycine max (L.) Merr., about 75 sterility mutants have been identified and most of them have been mapped to chromosomes. Mapping results have shown that some chromosomal regions are hotspots for fertility genes. Fine mapping of some of the male-sterile, female-fertile mutants and male-sterile, femalesterile mutants resulted in identification of candidate genes for fertility. Sequence comparisons further helped in locating a few putative candidates. A CACTA- like transposable element that is responsible for ...


Analysis Of Iron Transporters In The Soybean (Glycine Max (L.) Merr.) Genome, Dan Stribe Jan 2012

Analysis Of Iron Transporters In The Soybean (Glycine Max (L.) Merr.) Genome, Dan Stribe

Graduate Theses and Dissertations

Iron plays a significant role in numerous cellular functions. Improved uptake and efficiency of use can have strong positive effects on the health of plants, animals and humans. Major players in iron homeostasis are the Iron-Regulated Transporters (IRT). The purpose of this research is to identify putative Iron-Regulated Transporter genes in soybean, structurally characterize these genes and examine their relational phylogeny. Additionally, gene sequence data and gene expression patterns will be considered to differentiate each gene's potential role in iron homeostasis of soybean. In this study, we identified fourteen soybean gene models with significant predicted protein similarity to A ...


A Type Ii Metacaspase Interacts With Rps1-K-2 In Soybean And Analysis Of The Soybean Metacaspase Gene Family, James Andrew Baskett Jan 2012

A Type Ii Metacaspase Interacts With Rps1-K-2 In Soybean And Analysis Of The Soybean Metacaspase Gene Family, James Andrew Baskett

Graduate Theses and Dissertations

Soybean suffers from the root and stem rot disease caused by the oomycete pathogen, Phytophthora sojae. A series of Rps genes confer Phytophthora resistance, and have been utilized in fighting this serious disease in soybean. Earlier we reported the cloning of the coiled coil-nucleotide binding-leucine rich repeat gene, Rps1-k-2. Here we report the identification of a type II metacaspase, Gmmc11 that interacted with the coiled-coil (CC) domain of Rps1-k-2 in vivo in yeast. The interaction was verified by conducting in vitro pull down assays in unpublished work. RNA interference of Gmmc11 in cotyledons resulted in loss of Phytophthora resistance. Gmmc11 ...


Fusarium Species Infecting Soybean Roots: Frequency, Aggressiveness, Yield Impact And Interaction With The Soybean Cyst Nematode, Maria Mercedes Diaz Arias Jan 2012

Fusarium Species Infecting Soybean Roots: Frequency, Aggressiveness, Yield Impact And Interaction With The Soybean Cyst Nematode, Maria Mercedes Diaz Arias

Graduate Theses and Dissertations

Fusarium root rot is a widespread and common disease in soybean. Several Fusarium species have been reported to infect soybean roots but their occurrence, aggressiveness and impact on yield have not been quantified. To gain a better understanding of the importance of root-infecting Fusarium species in soybean productivity in Iowa, a three year root survey, and greenhouse and field experiments were conducted in order to accomplish the following objectives, 1) characterize the frequency of Fusarium species associated with soybean roots from Iowa soybean fields, 2) determine aggressiveness of predominant Fusarium species towards soybean, 3) estimate the impact of Fusarium species ...