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Iowa State University

Plant Sciences

1992

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Diversity Among Rhizobia Effective With Robinia Pseudoacacia L, Janet Mccray-Batzli, William R. Graves, Peter Van Berkum Jul 1992

Diversity Among Rhizobia Effective With Robinia Pseudoacacia L, Janet Mccray-Batzli, William R. Graves, Peter Van Berkum

William R Graves

The diversity of rhizobia that form symbioses with roots of black locust (Robinia pseudoacacia L.), an economically important leguminous tree species, was examined by inoculating seedling root zones with samples of soil collected from the United States, Canada, and China. Bacteria were isolated from nodules, subcultured, and verified to be rhizobia. The 186 isolates varied significantly in their resistance to antibiotics and NaCl, their growth on different carbohydrates, and their effect on the pH of culture media. Most isolates showed intermediate antibiotic resistance, the capacity to use numerous carbohydrates, and a neutral to acid pH response. Isolates had greater similarity …


Forage Quality Of Alfalfa Protected By Resistance To Bacterial Leaf Spot, Andrew W. Lenssen, E. L. Sorensen, G. L. Posler, D. L. Stuteville Jan 1992

Forage Quality Of Alfalfa Protected By Resistance To Bacterial Leaf Spot, Andrew W. Lenssen, E. L. Sorensen, G. L. Posler, D. L. Stuteville

Andrew W. Lenssen

Bacterial leaf spot (BLS), caused by Xanthomonas campestris pv. arfalfae Dye (Xca ), can prevent stand establishment and decrease the yield and persistence of alfalfa (Medicago sativa L.) in the central USA. Previous studies have shown that BLS inoculation may affect concentrations of monophenolic compounds and thus alter the forage quality of alfalfa. In a controlled environment, comparisons were made of forage quality, including phenolic monomers, of alfalfa cv. Kanza (susceptible) and KS76 germplasm (derived from Kanza, resistant) with and without inoculation with Xca. The cultivarx inoculation interactions were non-significant (P> 0.05 ) for all parameters determined for leaves and …