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Iowa State University

Plant Sciences

Graduate Theses and Dissertations

Protein

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13c-Metabolic Flux Analysis Of Soybean Somatic Embryos For Identification Of Metabolic Control Points In Developing Seed, Quyen Xuan Truong Dec 2012

13c-Metabolic Flux Analysis Of Soybean Somatic Embryos For Identification Of Metabolic Control Points In Developing Seed, Quyen Xuan Truong

Graduate Theses and Dissertations

Of the world's major agronomic food crops soybeans rank highest in protein content (~40% Dwt) while also containing significant quantities of oil (~20% Dwt). Based on these unique characteristics soy has become a mainstay in world agriculture, providing a protein source for livestock and human nutrition (68% of global vegetable protein meal consumption in 2011) as well as a primary source of vegetable oil (28% of global vegetable oil consumption in 2011; http://www.soystats.com/2012). The value of soy is therefore found both in its oil and protein content and increasing the content of both is therefore ...


Marker-Assisted Selection For Elevated Concentrations Of The Α′ Subunit Of Β-, Sheilah Emilie Oltmans-Deardorff Jan 2012

Marker-Assisted Selection For Elevated Concentrations Of The Α′ Subunit Of Β-, Sheilah Emilie Oltmans-Deardorff

Graduate Theses and Dissertations

Soybean [Glycine max (L.) Merr.] contains two major storage proteins, glycinin and β-conglycinin (BC). Glycinin accounts for approximately 40% and BC approximately 25% of total soybean seed protein (Nielsen et al., 1989). Glycinin is controlled by five genes coding for the acidic (A) and basic (B) subunits: Gy1, Gy2, Gy3, Gy4, and Gy5 (Cho et al., 1989; Nielsen et al., 1989). BC consists of gene families that encode the three major subunits α, α′, and β. The relative concentrations of the BC subunits are about 45% α, 35% α′, and 20% β (Maruyama et al., 1999). Soybean cultivars were discovered ...


Agronomic, Seed Composition And Molecular Characteristics Of Soybean Lines With Novel Genes For Modified Glycinin And Beta-Conglycinin Content, Jonathan Jenkinson Jan 2009

Agronomic, Seed Composition And Molecular Characteristics Of Soybean Lines With Novel Genes For Modified Glycinin And Beta-Conglycinin Content, Jonathan Jenkinson

Graduate Theses and Dissertations

Soybean [Glycine max (L.) Merr.] genotypes with increased beta-conglycinin (BC) and reduced glycinin (Gly) may be beneficial to food product quality and to human health. A soybean line, B2G2, developed in Japan, has the recessive alleles gy1, gy2, gy3, gy4, and gy5 that control Gly content. Its seed protein has about 50 g kg-1 BC and 0 g kg-1 Gly. Monsanto Co. has used B2G2 to develop lines with different combinations of recessive and wild-type alleles at the five Gy loci. The first objective of this research was to determine the influence of different combinations of the gy and Gy ...


Evaluation Fo Frost Damage In Seed Corn (Zea Mays): With Special Emphasis On Seed Composition And Moisture Content, Heather Ann Hall Jan 2009

Evaluation Fo Frost Damage In Seed Corn (Zea Mays): With Special Emphasis On Seed Composition And Moisture Content, Heather Ann Hall

Graduate Theses and Dissertations

Fall frost is a serious concern to seed corn (Zea mays L.) producers in the Midwest. A frost event that happens prior to seed maturity can cause physical, biochemical, and physiological changes in the seed. These changes lead to decreased vigor and viability resulting in revenue loss for seed corn producers. Genetics and seed maturity greatly influence seed tolerance to frost damage. The central hypothesis of this study is that differences in frost tolerance can occur among the same genotype and these differences may be the result of varying seed oil and protein contents. Information gathered from this study may ...


Horticultural Evaluation Of Zein-Based Bioplastic Containers, Matthew Steven Helgeson Jan 2009

Horticultural Evaluation Of Zein-Based Bioplastic Containers, Matthew Steven Helgeson

Graduate Theses and Dissertations

Environmental problems caused by petroleum-based plastics have led to interest in alternatives made from biodegradable polymers (bioplastics), but little effort has been made to evaluate horticultural containers made from these materials. Containers were fabricated from polymers of the corn (Zea mays L.) protein, zein. My first objective was to determine the longevity of zein-based containers under conditions typical of horticultural production. Zein containers of two wall thicknesses were filled with either a peat-based, soilless potting substrate or coarse perlite, and they were irrigated every 2 or 4 days. After 10 weeks, weight loss of containers was determined as a measure ...