Open Access. Powered by Scholars. Published by Universities.®

Digital Commons Network

Open Access. Powered by Scholars. Published by Universities.®

PDF

Iowa State University

Plant Sciences

Graduate Theses and Dissertations

Autophagy

Publication Year

Articles 1 - 5 of 5

Full-Text Articles in Entire DC Network

A Tale Of Regulation: Analysis Of The Role Of Snrk1 And Glutathione In Autophagy In Arabidopsis Thaliana, Junmarie Soto-Burgos Jan 2018

A Tale Of Regulation: Analysis Of The Role Of Snrk1 And Glutathione In Autophagy In Arabidopsis Thaliana, Junmarie Soto-Burgos

Graduate Theses and Dissertations

Autophagy is a degradation process in which cells break down and recycle their cytoplasmic contents when subjected to environmental stress or during development. Upon activation of autophagy, a double membrane vesicle called the autophagosome forms around the cargo and delivers it to the vacuole/lysosome for degradation. In mammals, regulation of autophagy has been extensively studied. In plants, key regulators of autophagy have been identified, but their upstream components are poorly understood. This dissertation summarizes my efforts in studying the regulation of autophagy in Arabidopsis thaliana.

AMPK in animals, and its yeast homolog Snf1, are positive regulators of autophagy. The ...


To Grow Or Survive: Plants Modulate Brassinosteroid-Regulated Transcription Factor Bes1 During Drought To Balance Growth And Stress Responses, Trevor Nolan Jan 2018

To Grow Or Survive: Plants Modulate Brassinosteroid-Regulated Transcription Factor Bes1 During Drought To Balance Growth And Stress Responses, Trevor Nolan

Graduate Theses and Dissertations

Understanding how plants balance growth and stress responses is essential to optimize crop yield in an ever-changing environment. Brassinosteroids (BRs) regulate plant growth and stress responses, including that of drought. BRs signal to control the activities of the BES1/BZR1 family transcription factors (TFs), which in turn mediate the expression of more than 5,000 BR-responsive genes. The network through which BES1 regulates the large number of target genes and the factors that modulate BES1 during stress are only beginning to be understood. In this thesis, I investigated several mechanisms that converge on BES1 to balance BR-regulated growth and stress ...


Autophagy- And Rns2-Mediated Mechanism Of Ribosomal Rna Degradation In Arabidopsis Thaliana, Stephanie C. Morriss Jan 2016

Autophagy- And Rns2-Mediated Mechanism Of Ribosomal Rna Degradation In Arabidopsis Thaliana, Stephanie C. Morriss

Graduate Theses and Dissertations

Ribosomal RNA degradation is necessary to remove nonfunctional rRNA and maintain homeostasis through degradation of aging or normal rRNA. While pathways for surveillance of nonfunctional rRNA have been characterized, mechanisms of degradation of normal rRNA are not yet well understood. Ribosomal RNA, the majority of cellular RNA, forms a massive resource sink. These studies examine the mechanism of degradation of rRNA in Arabidopsis and the effects of disruption of this process through mutation of key genes.

Ribosomal RNA in many organisms, including yeast, zebrafish, Tetrahymena and Arabidopsis, is degraded by the RNase T2 family of enzymes, a family highly conserved ...


Analysis Of The Role Of Rns2 And Autophagy In The Turnover Of Ribosomal Rna In Arabidopsis Thaliana, Brice Edward Floyd Jan 2015

Analysis Of The Role Of Rns2 And Autophagy In The Turnover Of Ribosomal Rna In Arabidopsis Thaliana, Brice Edward Floyd

Graduate Theses and Dissertations

The degradation and turnover of cellular components is essential for biological systems to remove damaged components and maintain functional intracellular contents. Autophagy is a vesicle-mediated process by which cellular contents can be degraded through trafficking to the lysosome or vacuole where lytic processes occur. The majority of a cell’s RNA and large quantities of its protein can be found in ribosomes. Ribosomes represent a significant metabolic investment. However, little is known about the turnover of these highly abundant ribonuclear organelles in plants.

RNase T2 enzymes represent a widely conserved class of ribonucleases present in the genomes of nearly all ...


Analysis Of The Function And The Regulation Of Autophagy In Arabidopsis Thaliana, Yimo Liu Jan 2012

Analysis Of The Function And The Regulation Of Autophagy In Arabidopsis Thaliana, Yimo Liu

Graduate Theses and Dissertations

Plants have developed sophisticated mechanisms to survive under adverse growth conditions. Autophagy is activated in response to multiple abiotic stresses, pathogen infection and senescence in plants. Upon induction of autophagy, portions of cytoplasm are engulfed by double membrane structures termed autophagosomes and are delivered to vacuole for degradation. Over the past decade, the identification of many autophagy-related genes in plants has greatly enhanced our molecular and physiological understanding of plant autophagy. However, the upstream regulatory components in the autophagy pathway and the function of autophagy under various stresses are still unclear. This thesis summarizes my efforts in studying the function ...