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Iowa State University

Plant Sciences

Ecology, Evolution and Organismal Biology Publications

Brassica

Publication Year

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Cenh3 Evolution In Diploids And Polyploids Of Three Angiosperm Genera, Rick E. Masonbrink, Joseph P. Gallagher, Josef J. Jareczek, Simon Renny-Byfield, Corrinne E. Grover, Lei Gong, Jonathan F. Wendel Dec 2014

Cenh3 Evolution In Diploids And Polyploids Of Three Angiosperm Genera, Rick E. Masonbrink, Joseph P. Gallagher, Josef J. Jareczek, Simon Renny-Byfield, Corrinne E. Grover, Lei Gong, Jonathan F. Wendel

Ecology, Evolution and Organismal Biology Publications

Centromeric DNA sequences alone are neither necessary nor sufficient for centromere specification. The centromere specific histone, CenH3, evolves rapidly in many species, perhaps as a coevolutionary response to rapidly evolving centromeric DNA. To gain insight into CenH3 evolution, we characterized patterns of nucleotide and protein diversity among diploids and allopolyploids within three diverse angiosperm genera, Brassica, Oryza, and Gossypium (cotton), with a focus on evidence for diversifying selection in the various domains of the CenH3 gene. In addition, we compare expression profiles and alternative splicing patterns for CenH3 in representatives of each genus. All three genera retain both duplicated CenH3 ...


Analysis Of Bulked And Redundant Accessions Of Brassica Germplasm Using Assignment Tests Of Microsatellite Markers, Von Mark V. Cruz, John D. Nason, Richard Luhman, Laura F. Marek, Randy C. Shoemaker, E. Charles Brummer, Candice A. C. Gardner Dec 2006

Analysis Of Bulked And Redundant Accessions Of Brassica Germplasm Using Assignment Tests Of Microsatellite Markers, Von Mark V. Cruz, John D. Nason, Richard Luhman, Laura F. Marek, Randy C. Shoemaker, E. Charles Brummer, Candice A. C. Gardner

Ecology, Evolution and Organismal Biology Publications

This study was conducted to determine if Brassica germplasm bulks created and maintained by the USDA-ARS North Central Plant Introduction Station (NCRPIS) were made with genetically indistinguishable component accessions and to examine newly identified putative duplicate accessions to determine if they can be bulked. Using ten microsatellite primer pairs, we genotyped two bulks of B. rapa L. ssp. dichotoma (Roxb.) Hanelt comprising four accessions and three bulks of B. rapa L. ssp. trilocularis (Roxb.) Hanelt comprising fourteen accessions, as well as four pairs of putatively duplicate accessions of B.‚ź£napus L. Assignment tests on ten individual plants per accession were ...