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Jncml-Like, An Ef-Hand Motif-Containing Gene Seasonally Upregulated In The Transition Zone Of Black Walnut (Juglans Nigra L.), Priyanka Surana, Zhonglian Huang, Daisuke Kihara, Richard Meilan, Keith Woeste

Priyanka Surana

The economic value of a black walnut (Juglans nigra L.) tree is strongly determined by the quality and quantity of darkly colored heartwood in its stem. To understand the regulation of heartwood formation, we analyzed the region of heartwood formation in walnut stems (i.e., the transition zone, TZ) for the expression of 80 ESTs. Semi-quantitative RT-PCR and real-time PCR was performed to detect expression changes of candidate genes in the TZ and sapwood of trees harvested in summer and fall. Results revealed that the transcript of a clone containing two presumed EF-hand motifs was expressed at higher levels in the …