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Rapid Biocompatibility Analysis Of Materials Via In Vivo Fluorescence Imaging Of Mouse Models, Kaitlin M. Bratlie, Tram T. Dang, Stephen Lyle, Matthias Nahrendorf, Ralph Weissleder, Robert Langer, Daniel G. Anderson

Kaitlin M. Bratlie

Background: Many materials are unsuitable for medical use because of poor biocompatibility. Recently, advances in the high throughput synthesis of biomaterials has significantly increased the number of potential biomaterials, however current biocompatibility analysis methods are slow and require histological analysis. Methodology/Principal Findings: Here we develop rapid, non-invasive methods for in vivo quantification of the inflammatory response to implanted biomaterials. Materials were placed subcutaneously in an array format and monitored for host responses as per ISO 10993-6: 2001. Host cell activity in response to these materials was imaged kinetically, in vivo using fluorescent whole animal imaging. Data captured using whole animal …