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Iowa State University

Agricultural Science

3' untranslated region

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Cation-Dependent Folding Of 30 Cap-Independent Translation Elements Facilitates Interaction Of A 17-Nucleotide Conserved Sequence With Eif4g, Jelena Jeremic Kraft, Krzysztof Treder, Mariko Sada Peterson, W. Allen Miller Jan 2014

Cation-Dependent Folding Of 30 Cap-Independent Translation Elements Facilitates Interaction Of A 17-Nucleotide Conserved Sequence With Eif4g, Jelena Jeremic Kraft, Krzysztof Treder, Mariko Sada Peterson, W. Allen Miller

Plant Pathology and Microbiology Publications

The 3'-untranslated regions of many plant viral RNAs contain cap-independent translation elements (CITEs) that drive translation initiation at the 5'-end of the mRNA. The barley yellow dwarf virus-like CITE (BTE) stimulates translation by binding the eIF4G subunit of translation initiation factor eIF4F with high affinity. To understand this interaction, we characterized the dynamic structural properties of the BTE, mapped the eIF4G-binding sites on the BTE and identified a region of eIF4G that is crucial for BTE binding. BTE folding involves cooperative uptake of magnesium ions and is driven primarily by charge neutralization. Footprinting experiments revealed that functional eIF4G ...


The 3′ Cap-Independent Translation Element Of Barley Yellow Dwarf Virus Binds Eif4f Via The Eif4g Subunit To Initiate Translation, Krzysztof Treder, Elizabeth L. Pettit Kneller, Edwards M. Allen, Zhaohui Wang, Karen S. Browning, W. Allen Miller Jan 2008

The 3′ Cap-Independent Translation Element Of Barley Yellow Dwarf Virus Binds Eif4f Via The Eif4g Subunit To Initiate Translation, Krzysztof Treder, Elizabeth L. Pettit Kneller, Edwards M. Allen, Zhaohui Wang, Karen S. Browning, W. Allen Miller

Plant Pathology and Microbiology Publications

The 3′ cap-independent translation element (BTE) of Barley yellow dwarf virus RNA confers efficient translation initiation at the 5′ end via long-distance base pairing with the 5′-untranslated region (UTR). Here we provide evidence that the BTE functions by recruiting translation initiation factor eIF4F. We show that the BTE interacts specifically with the cap-binding initiation factor complexes eIF4F and eIFiso4F in a wheat germ extract (wge). In wge depleted of cap-interacting factors, addition of eIF4F (and to a lesser extent, eIFiso4F) allowed efficient translation of an uncapped reporter construct (BLucB) containing the BTE in its 3′ UTR. Translation of BLucB ...


Trans Regulation Of Cap-Independent Translation By A Viral Subgenomic Rna, Ruizhong Shen, Aurélie Mamisoa Rakotondrafara, W. Allen Miller Oct 2006

Trans Regulation Of Cap-Independent Translation By A Viral Subgenomic Rna, Ruizhong Shen, Aurélie Mamisoa Rakotondrafara, W. Allen Miller

Plant Pathology and Microbiology Publications

Many positive-strand RNA viruses generate 3′-coterminal subgenomic mRNAs to allow translation of 5′-distal open reading frames. It is unclear how viral genomic and subgenomic mRNAs compete with each other for the cellular translation machinery. Translation of the uncapped Barley yellow dwarf virus genomic RNA (gRNA) and subgenomic RNAI (sgRNAI) is driven by the powerful cap-independent translation element (BTE) in their 3′ untranslated regions (UTRs). The BTE forms a kissing stem-loop interaction with the 5′ UTR to mediate translation initiation at the 5′ end. Here, using reporter mRNAs that mimic gRNA and SgRNA1, we show that the abundant sgRNA2 ...


A Potential Mechanism For Selective Control Of Cap-Independent Translation By A Viral Rna Sequence In Cis And In Trans, Shanping Wang, Liang Guo, Edwards Allen, W. Allen Miller Jan 1999

A Potential Mechanism For Selective Control Of Cap-Independent Translation By A Viral Rna Sequence In Cis And In Trans, Shanping Wang, Liang Guo, Edwards Allen, W. Allen Miller

Plant Pathology and Microbiology Publications

Highly efficient cap-independent translation initiation at the 5'- proximal AUG is facilitated by the 3' translation enhancer sequence (3'TE) located near the 3' end of barley yellow dwarf virus (BYDV) genomic RNA. The role of the 3'TE in regulating viral translation was examined. The 3'TE is required for translation and thus replication of the genomic RNA that lacks a 5' cap (Allen et al., 1999, Virology 253:139-144). Here we show that the 3'TE also mediates translation of uncapped viral subgenomic mRNAs (sgRNA1 and sgRNA2). A 109-nt viral sequence is sufficient for 3'TE activity in ...