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Articles 1 - 9 of 9
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Inhalation Toxicity Of (1→3)-Β-D-Glucan: Recent Advances, Birgitts Fogelmark, Margarets Sjöstrand, David Williams, Ragnar Rylander
Inhalation Toxicity Of (1→3)-Β-D-Glucan: Recent Advances, Birgitts Fogelmark, Margarets Sjöstrand, David Williams, Ragnar Rylander
ETSU Faculty Works
To investigate the effects of (1→3)-β-D-glucan after inhalation, animals were exposed to different forms of glucan and the number of lung lavage cells was determined 24 h after exposure. None of the different forms assayed caused any increase in cell numbers. In animals exposed to endotoxin, all types of cells were increased after 24 h. A simultaneous exposure to curdlan reduced this increase in a dose-related fashion. The results suggest that (1→3)-β-D-glucan-related acute injury to the lung is induced by mechanisms other than those induced by inflammagenic agents such as endotoxin.
Overview Of (1→3)-Β-D-Glucan Immunobiology, David L. Williams
Overview Of (1→3)-Β-D-Glucan Immunobiology, David L. Williams
ETSU Faculty Works
Glucans are (1→3)-β-D-glucose polymers that are found in the cell wall of fungi, bacteria and plants. Glucans are known to stimulate humoral and cell-mediated immunity in humans and animals. In addition to the potent immune stimulatory effects of (1→3)-β-D-glucans, there are a number of toxicological effects associated with exposure to the water-insoluble, microparticulate form of the polymer. Recent investigations have suggested a potential role for (1→3)-β-D-glucans in inhalational toxicity. Specifically, (1→3)-β-D-glucans have been implicated in the symptomatology associated with 'sick building' syndrome. The mechanisms by which (1→3)-β- D-glucans mediate immune stimulation and, perhaps, toxicity are currently under investigation. It is …
An Animal Cell Mutant With A Deficiency In Acyl/Alkyl-Dihydroxyacetone- Phosphate Reductase Activity. Effects On The Biosynthesis Of Ether-Linked And Diacyl Glycerolipids, Paul F. James, Andrew C. Lake, Amiya K. Hajra, Leslie K. Larkins, Mitchell Robinson, F. Gregory Buchanan, Raphael A. Zoeller
An Animal Cell Mutant With A Deficiency In Acyl/Alkyl-Dihydroxyacetone- Phosphate Reductase Activity. Effects On The Biosynthesis Of Ether-Linked And Diacyl Glycerolipids, Paul F. James, Andrew C. Lake, Amiya K. Hajra, Leslie K. Larkins, Mitchell Robinson, F. Gregory Buchanan, Raphael A. Zoeller
ETSU Faculty Works
In the accompanying paper (James, P. F., and Zoeller, R. A. (1997) J. Biol. Chem. 272, 23532-23539), we reported the isolation of a series of mutants from the fibroblast-like cell line, CHO-K1, that are deficient in the incorporation of the long chain fatty alcohol, hexadecanol, into complex lipids. All but one of these mutants, FAA.K1B, were deficient in long-chain- fatty alcohol oxidase (FAO) activity. We have further characterized this FAO+ isolate. FAA.K1B cells displayed a 40% decrease in [9,103H]hexadecanol uptake when compared with the parent strain. Although incorporation of hexadecanol into the phospholipid fraction was decreased by 52%, the cells …
Comparison Of The Dna Association Kinetics Of The Lacy Repressor Tetramet, Its Dimeric Mutant Laci(Adi), And The Native Dimeric Gal Repressor, Mark Hsieh, Michael Brenowitz
Comparison Of The Dna Association Kinetics Of The Lacy Repressor Tetramet, Its Dimeric Mutant Laci(Adi), And The Native Dimeric Gal Repressor, Mark Hsieh, Michael Brenowitz
ETSU Faculty Works
The rates of association of the tetrameric Lacy repressor (LacI), dimeric LacI(adi) (a deletion mutant of LacI), and the native dimeric Gal repressor (GalR) to DNA restriction fragments containing a single specific site were investigated using a quench-flow DNase I 'foot-printing' technique. The dimeric proteins, LacI(adi) and GalR, and tetrameric LacI possess one and two DNA binding sites, respectively. The nanomolar protein concentrations used in these studies ensured that the state of oligomerization of each protein was predominantly either dimeric or tetrameric, respectively. The bimolecular association rate constants (k(a)) determined for the LacI tetramer exceed those of the dimeric proteins. …
Auditory Filters Measured At Neighboring Center Frequencies, Marc A. Fagelson, C. A. Champlin
Auditory Filters Measured At Neighboring Center Frequencies, Marc A. Fagelson, C. A. Champlin
ETSU Faculty Works
Auditory filters were derived in 20 normal-hearing human listeners at center frequencies (CFs) of 913, 1095, 3651, and 4382 Hz using the roex (p,r) method. Comparisons were made between slopes of the filters' skirts at the neighboring CFs with filter output levels of 45 and 70 dB. The same comparisons were made with regard to filter equivalent rectangular bandwidth (ERB). In the 1000-Hz region, the low-frequency slopes (Pl) of filters centered at 913 and 1095 Hz were significantly correlated at both stimulus levels, while the high-frequency slopes (Pu) were similar only at the high test level. In the 4000-Hz region, …
In Vitro Assay And Characterization Of The Farnesylation-Dependent Prelamin A Endoprotease, Fusun Kilic, Marguerite B. Dalton, Sarah K. Burrell, John P. Mayer, Scott D. Patterson, Michael Sinensky
In Vitro Assay And Characterization Of The Farnesylation-Dependent Prelamin A Endoprotease, Fusun Kilic, Marguerite B. Dalton, Sarah K. Burrell, John P. Mayer, Scott D. Patterson, Michael Sinensky
ETSU Faculty Works
The 72-kDa nuclear lamina protein lamin A is synthesized as a 74-kDa farnesylated precursor. Conversion of this precursor to mature lamin A appears to be mediated by a specific endoprotease. Prior studies of overexpressed wild-type and mutant lamin A proteins in cultured cells have indicated that the precursor possesses the typical carboxyl-terminal S- farnesylated, cysteine methyl ester and that farnesylation is required for endoproteolysis to occur. In this report, we describe the synthesis of an S- farnesyl, cysteinyl methyl ester peptide corresponding to the carboxyl- terminal 18 amino acid residues of human prelamin A. This peptide acts as a substrate …
Beryllium Fluoride And Phalloidin Restore Polymerizability Of A Mutant Yeast Actin (V266g,L267g) With Severely Decreased Hydrophobicity In A Subdomain 3/4 Loop, Bing Kuang, Peter A. Ruhenstein
Beryllium Fluoride And Phalloidin Restore Polymerizability Of A Mutant Yeast Actin (V266g,L267g) With Severely Decreased Hydrophobicity In A Subdomain 3/4 Loop, Bing Kuang, Peter A. Ruhenstein
ETSU Faculty Works
Holmes proposed that in F-actin, hydrophobic residues in a subdomain 3/4 loop interact with a hydrophobic pocket on the opposing strand resulting in helix stabilization. We have determined how a decreased hydrophobicity of this plug affects yeast actin function. Cells harboring only the V266G, V266D, V266F, L267G, L269D, or L269K actins appear normal, although V266G cells display an altered budding pattern. However, V266G, L267G (GG) double mutant cells are cold-sensitive with randomly oriented thick actin assemblies seen in rhodamine phalloidin-stained GG cells. V266D actin polymerizes slower than wild-type actin at room temperature. At 4 °C, not only is polymerization slowed, …
Immunologic Investigations Of T-Cell Regulation Of Human Ige Antibody Secretion And Allergic Responses, David M. Essayan, Guha Krishnaswamy, Shau Ku Huang
Immunologic Investigations Of T-Cell Regulation Of Human Ige Antibody Secretion And Allergic Responses, David M. Essayan, Guha Krishnaswamy, Shau Ku Huang
ETSU Faculty Works
The pathophysiology of allergic disease is multifactorial, involving an intricate network of interactions among cells, mediators, and cytokines. Substantial progress has been made in defining the role of antigen-specific T cells and cytokines in the regulation of immunoglobulin E (IgE) synthesis and the atopic diseases. The development of antigen-specific T-cell lines and clones has facilitated efforts to characterize human T-cell subsets and their cytokine repertoires. Molecular methods currently available include techniques for the quantitative analysis of cytokine gene expression and secretion from activated T cells ex vivo as well as in tissues. The availability of these newly developed techniques has …
All About Me: Promoting Reading And Writing Through Creating Autobiographical Posters, Edward J. Dwyer
All About Me: Promoting Reading And Writing Through Creating Autobiographical Posters, Edward J. Dwyer
ETSU Faculty Works
No abstract provided.