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Transcriptional Activation Of The Cholesterol 7Α-Hydroxylase Gene (Cyp7a) By Nuclear Hormone Receptors, Maurizio Crestani, Azita Sadeghpour, Diane Stroup, Giovanni Galli, John Y.L. Chiang
Transcriptional Activation Of The Cholesterol 7Α-Hydroxylase Gene (Cyp7a) By Nuclear Hormone Receptors, Maurizio Crestani, Azita Sadeghpour, Diane Stroup, Giovanni Galli, John Y.L. Chiang
ETSU Faculty Works
The gene encoding cholesterol 7α-hydroxylase (CYP7A), the rate-limiting enzyme in bile acid synthesis, is transcriptionally regulated by bile acids and hormones. Previously, we have identified two bile acid response elements (BARE) in the promoter of the CYP7A gene. The BARE II is located in nt - 149/-118 region and contains three hormone response element (HRE)-like sequences that form two overlapping nuclear receptor binding sites. One is a direct repeat separated by one nucleotide DR1 (-146-TGGACTtAGTTCA-134) and the other is a direct repeat separated by five nucleotides DR5 (-139- AGTTCAaggccGGGTAA-123). Mutagenesis of these HRE sequences resulted in lower transcriptional activity of …
Tyrosine Phosphorylation Of C-Erbb-2 Is Regulated By The Cellular Form Of Prostatic Acid Phosphatase In Human Prostate Cancer Cells, Tzu Ching Meng, Ming Fong Lin
Tyrosine Phosphorylation Of C-Erbb-2 Is Regulated By The Cellular Form Of Prostatic Acid Phosphatase In Human Prostate Cancer Cells, Tzu Ching Meng, Ming Fong Lin
ETSU Faculty Works
Human prostatic acid phosphatase (PAcP) is a prostate epithelium- specific differentiation antigen. In prostate carcinomas, the cellular PAcP is decreased. We investigated its functional role in these cells. Several lines of evidence support the hypothesis that cellular PAcP functions as a neutral protein-tyrosine phosphatase and is involved in regulating prostate cell growth. In this study, we identify its in vivo substrate. Our results demonstrated that, in different human prostate cancer cell lines, the phosphotyrosine (Tyr(P)) level of a 185-kDa phosphoprotein (pp185) inversely correlates with the cellular activity of PAcP. On SDS-PAGE, this pp185 comigrates with the c-ErbB-2 oncoprotein. Immunodepletion experiments …
Oleate Potentiates Oxysterol Inhibition Of Transcription From Sterol Regulatory Element-1-Regulated Promoters And Maturation Of Sterol Regulatory Element-Binding Proteins, Douglas P. Thewke, Sankhavaram R. Panini, Michael Sinensky
Oleate Potentiates Oxysterol Inhibition Of Transcription From Sterol Regulatory Element-1-Regulated Promoters And Maturation Of Sterol Regulatory Element-Binding Proteins, Douglas P. Thewke, Sankhavaram R. Panini, Michael Sinensky
ETSU Faculty Works
Activation of genes containing SRE-1 (sterol regulatory element 1) sequences is known to be under the regulation of sterols through modulation of the proteolytic maturation of SREBPs (SRE-1-binding proteins). Previous work has demonstrated SREBP-mediated transcriptional activation of genes encoding enzymes of sterol and fatty acid biosynthesis. Because synthesis of both sterols and C18 fatty acids are required for cell growth, in the absence of exogenous supplements of these lipids, we examined the hypothesis that fatty acid can also be regulatory in SREBP maturation. Our data indicate that C18 fatty acids can potentiate the biological activities of a typical, regulatory sterol: …
Evidence For A High Affinity, Saturable, Prenylation-Dependent P21(Ha- Ras) Binding Site In Plasma Membranes, Afzal A. Siddiqui, John R. Garland, Marguerite B. Dalton, Michael Sinensky
Evidence For A High Affinity, Saturable, Prenylation-Dependent P21(Ha- Ras) Binding Site In Plasma Membranes, Afzal A. Siddiqui, John R. Garland, Marguerite B. Dalton, Michael Sinensky
ETSU Faculty Works
Oncogenic p21(ras) proteins can only exert their stimulation of cellular proliferation when plasma membrane-associated. This membrane association has an absolute requirement for post-translational modification with isoprenoids. The mechanism by which isoprenoids participate in the specific association of p21(ras) with plasma membranes is the subject of this report. We present in vitro evidence for a plasma membrane binding protein for p21(ras) that can recognize the isoprenoid substituent and, therefore, may facilitate the localization of p21(ras).
Making Sturdy Cloth-Bound Books Using Heat-N-Bond Ultra, Edward J. Dwyer, Evelyn Dwyer
Making Sturdy Cloth-Bound Books Using Heat-N-Bond Ultra, Edward J. Dwyer, Evelyn Dwyer
ETSU Faculty Works
The road to competence in writing is best paved with joyful experiences, especially early experiences with writing in the elementary school. One such experience is writing a story and making a beautiful cloth-bound book. Materials needed, which are easily obtained, are:(1) a commercially produced material called Heat-N-Bond (Ultra);(2) mat board or heavy weight poster board;(3)heavy weight paper in a variety of colors;(4) cloth in bright patterns or any other choice;(5) plain white photocopy paper;(6)long arm stapler; and (7) clothes irons. After assembling the book in 10 easy steps, photos or pictures from magazines or other sources maybe put on the …