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Identification Of Fluoxetine-Sert Interactions And Apo-Sert Studies Via Crosslinking Mass Spectrometry, Elizabeth Castellano
Identification Of Fluoxetine-Sert Interactions And Apo-Sert Studies Via Crosslinking Mass Spectrometry, Elizabeth Castellano
Electronic Theses and Dissertations
The serotonin transporter (SERT) is a member of the neurotransmitter sodium symporter family of transporters. SERT controls the magnitude and duration of serotonergic neurotransmission by facilitating the reuptake of serotonin back into the pre-synaptic neuron and is thus a target for antidepressants. Selective serotonin re-uptake inhibitors (SSRIs) such as fluoxetine, are commonly prescribed to treat depression. SSRIs act by blocking reuptake and prolonging serotonin signaling. However, significant problems regarding selectivity and mechanisms of action of these drugs remain unresolved. The structures of SERT and related transporters have been determined and serve as useful structural models. However, they are typically mutated …
Protein-Protein Interactions Of Human Mitochondrial Amidoxime-Reducing Component In Mammalian Cells, John Thomas
Protein-Protein Interactions Of Human Mitochondrial Amidoxime-Reducing Component In Mammalian Cells, John Thomas
Electronic Theses and Dissertations
Newly discovered pterin-molybdoenzymes mammalian proteins, mARC1, and mARC2, are though to activate pro-drug, metabolize mutated base pairs, and producing nitric oxide from nitrite. However, the physiological function of the mARC proteins remain unclear. It is hypothesized that identifying partner proteins through protein-protein interactions can provide evidence to their physiological function. This study aimed to further investigate the potential function of mARC proteins through the identification of protein-protein interaction in human cell lysate, utilizing co-immunoprecipitation, crosslinking reagent, and pull-down assays. Several proteins (e.g. mitogen-activated protein kinase 5, phosphatidylinositol 4-phospahte 3-kinase C2 domain-containing subunit beta, ADP/ATP translocase 1, and myotubularin) were identified …
Novel Solid-Phase Extraction Techniques For Biological And Environmental Analysis Using Isotope Dilution Mass Spectrometry, Andrew Boggess
Novel Solid-Phase Extraction Techniques For Biological And Environmental Analysis Using Isotope Dilution Mass Spectrometry, Andrew Boggess
Electronic Theses and Dissertations
Awareness and study of the ways in which the environment can interact with the personal genetics and epigenetics of an individual has grown substantially in recent years, resulting in the field of Exposomics. In an era of increasingly personalized medicine, novel techniques are necessary to ensure the accurate and sensitivity measurement of clinically and environmentally relevant molecules in biological and environmental samples. Addressing existing shortcomings cited in literature, methods were developed and optimized for the extraction, separation, mass analysis, and quantification of a suite of environmental organic pollutants in both biological and environmental samples, with the primary objective of improving …
Analysis Strategies For Bioactive, Polar Fatty Amides In Complex Samples, Erin Blaine Divito
Analysis Strategies For Bioactive, Polar Fatty Amides In Complex Samples, Erin Blaine Divito
Electronic Theses and Dissertations
Bioactive lipids are known to exert physiological effects and interact with neuroreceptors. Little is known about the bioregulation of primary fatty acid amides, though N-acyl glycines are thought to be their anabolic precursors. Chapter 1 details the current metabolic, physiological, and receptor interactions of primary fatty acid amides and their related congeners and reviews current strategies for isolation and detection. Chapter 2 outlines mass spectrometry collision induced fragmentation assignments pertaining to method development of multiple reaction monitoring detection of these species. Chromatographic separation methods of fatty acyls, with a focus on primary fatty acid amides and N-acyl glycines, are outlined …
Mass Spectrometry Applied To Problems In Lipid Biochemistry: Microchip Based Approach For Lipidomics Profiling And Analysis Of Lipid Metabolites By Lc-Ms/Ms, Tao Sun
Electronic Theses and Dissertations
Lipidomics and metabolomics are powerful tools for the examination of cellular metabolism and physiology. Methods for lipid analysis need to be developed that begin with small samples and do not overly dilute or disperse the sample in the separation process. Microchips provide a platform for interfacing lysis of small cell populations with on-chip solid phase extraction for isolating lipid samples to generate high quality mass spectra from very small samples. Chapter 1 of this dissertation presents a novel method for small scale lipidomics of bacterial cells by microchip based extraction coupled with untargeted profiling of glycerophospholipids using nanoelectrospray ionization mass …
Application Of Speciated Isotopes Dilution Mass Spectronmetry To The Assessment Of Human Health And Toxic Exposure, Timothy Fahrenholz
Application Of Speciated Isotopes Dilution Mass Spectronmetry To The Assessment Of Human Health And Toxic Exposure, Timothy Fahrenholz
Electronic Theses and Dissertations
Previous work by our research group demonstrated that quantitative chemical analysis of analytes, such as mercury and chromium species, in environmental matrices could be successfully carried out without using calibration curves and with correction for species interconversion by using EPA Method 6800A. This method encompasses isotope dilution mass spectrometry (IDMS) and speciated isotope dilution mass spectrometry (SIDMS), both of which are described in detail in chapter 1. Research described in this dissertation expands upon our earlier work by applying the method to the speciation of mercury in biological matrices, the speciation of glutathione in red blood cells and whole blood, …