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Kidney Development In Eleutherodactylus Coqui With Relation To Edema Syndrome, Seung Yun Lee
Kidney Development In Eleutherodactylus Coqui With Relation To Edema Syndrome, Seung Yun Lee
Electronic Theses and Dissertations
Vertebrates develop embryonic kidneys before the final adult kidney. Particularly for organisms with aquatic larvae such as frogs, the first embryonic kidney, the pronephros is essential for the larvae's survival. Those larvae with defective or without pronephroi exhibited pronounced edema. Edema is severe swelling of a body part as a result of fluid retention. To date, although the embryonic kidneys have been extensively studied in various model organisms, it has not been studied in Eleutherodactylus coqui. E. coqui is a direct developing frog and lacks a tadpole phase in its life cycle. I examined the kidney development in E. coqui …
Expresson Of The Endodermal Marker, Ecsox17, During Embryogenesis Of The Direct Developing Frog, Eleutherodactylus Coqui, Srikanth Singamsetty
Expresson Of The Endodermal Marker, Ecsox17, During Embryogenesis Of The Direct Developing Frog, Eleutherodactylus Coqui, Srikanth Singamsetty
Electronic Theses and Dissertations
In the model amphibian, Xenopus laevis, the HMG box transcription factor, Sox17, is expressed throughout the vegetal region that forms the endoderm. It is both necessary and sufficient for the endoderm formation. In order to study endoderm formation in the direct developing frog, Eleutherodactylus coqui, its Sox17 orthologue EcSox17 was cloned by Sean Willamson. To look at the expression of EcSox17, I designed primers and did RT-PCR on whole embryos. EcSox17 was expressed from cleavage to hatching of free-living froglet. EcSox17 RNA was present in the ovaries, an expression which has not been reported in any other vertebrate. Whole mount …