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A Baseline Study Of Chemical Parameters And Microbial Diversity Of Two Streams In The Ten Mile Creek Watershed In Southwestern Pennsylvania, Jennifer Rutter
A Baseline Study Of Chemical Parameters And Microbial Diversity Of Two Streams In The Ten Mile Creek Watershed In Southwestern Pennsylvania, Jennifer Rutter
Electronic Theses and Dissertations
As drilling for natural gas in the Marcellus Shale becomes increasingly prevalent, both human safety and environmental concerns have arisen. The aim of this study was to assemble an environmental baseline to make an accurate assessment of its possible impacts. Water samples and chemical parameters were collected from Bates Fork, a stream with Marcellus Shale drilling activity and Fonner Run, a sister stream with no drilling activity, on a monthly basis beginning in the summer of 2010. Bacterial DNA was also extracted from water collected at each site and then amplified using primers for the variable ribosomal internal transcribed spacer …
Enrichment, Characterization And Identification Of Microbial Communities Associated With Unconventional Shale Gas Production Water, J. Lucas Eastham
Enrichment, Characterization And Identification Of Microbial Communities Associated With Unconventional Shale Gas Production Water, J. Lucas Eastham
Electronic Theses and Dissertations
Unconventional natural gas extraction from the Marcellus Shale requires millions of gallons of water to fracture shale and release natural gas from the formation. This process produces water with high levels of total dissolved solids (TDS); and, efforts to recycle these fluids has stimulated microbial growth in produced water. The objective of this study was to analyze the ionic composition of and characterize microorganisms from Marcellus produced water samples. A semi-synthetic culture medium was designed with high TDS to enrich for halophilic microbes, which yielded robust cultures that were able to grow over a wide range of salinities. DNA extracted …