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Cloning And Expression Of Dazl In Rana Pipiens, Jamie Boorech
Cloning And Expression Of Dazl In Rana Pipiens, Jamie Boorech
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The DAZ gene on the human Y chromosome has been implicated in male fertility. Humans and other animals possess an autosomal version of this gene, referred to as DAZ-like or Dazl, which functions in gametogenesis and fertility. In order to expand our knowledge of Dazl, I cloned Dazl from the northern leopard frog Rana pipiens. To obtain the clone, I screened a R. pipiens ovary cDNA library with a RpDazl-specific PCR-generated radioactive probe. RpDazl, as determined by sequencing, is 3.5kb in length, with an 843 nucleotide open reading frame (ORF) and a 3' untranslated region (UTR) of 2635 nucleotides. At …