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Drosophila Fascin Mutants Are Rescued By Overexpression Of The Villin-Like Protein, Quail, Kelly Cant, Brenda A. Knowles, Shalina Mahajan-Miklos, Matthew Heintzelman, Lynn Cooley

Dartmouth Scholarship

Actin bundle assembly in specialized structures such as microvilli on intestinal epithelia and Drosophila bristles requires two actin bundling proteins. In these systems, the distinct biochemical properties and temporal localization of actin bundling proteins suggest that these proteins are not redundant. During Drosophila oogenesis, the formation of cytoplasmic actin bundles in nurse cells requires two actin bundling proteins, fascin encoded by the singed gene and a villin-like protein encoded by the quail gene. singed and quail mutations are fully recessive and each mutation disrupts nurse cell cytoplasmic actin bundle formation. We used P-element mediated germline transformation to overexpress quail in …

Gravitational Waves From Collapsing Vacuum Domains, Marcelo Gleiser, Ronald Roberts

Dartmouth Scholarship

The breaking of an approximate discrete symmetry, the final stages of a first order phase transition, or a postinflationary biased probability distribution for scalar fields are possible cosmological scenarios characterized by the presence of unstable domain wall networks. Combining analytical and numerical techniques, we show that the nonspherical collapse of these domains can be a powerful source of gravitational waves. We compute their contribution to the stochastic background of gravitational radiation and explore their observability by present and future gravitational wave detectors.

Hey, You Got Your Language In My Operating System!, Jon Howell, Mark Montague

Computer Science Technical Reports

Several projects in the operating systems research community suggest a trend of convergence among features once divided between operating systems and languages. We describe how partial evaluation and transformational programming systems apply to this trend by providing a general framework for application support, from compilation to run-time services. We contend that the community will no longer think of implementing a static collection of services and calling it an operating system; instead, this general framework will allow applications to be flexibly configured, and the ``operating system'' will simply be the application support that is supplied at run-time.

Snowflake: Spanning Administrative Domains, Jon Howell, David Kotz

Computer Science Technical Reports

Many distributed systems provide a ``single-system image'' to their users, so the user has the illusion that they are using a single system when in fact they are using many distributed resources. It is a powerful abstraction that helps users to manage the complexity of using distributed resources. The goal of the Snowflake project is to discover how single-system images can be made to span administrative domains. Our current prototype organizes resources in namespaces and distributes them using Java Remote Method Invocation. Challenging issues include how much flexibility should be built into the namespace interface, and how transparent the network …

The Motions Of Clusters Of Galaxies And The Dipoles Of The Peculiar Velocity Field, Riccardo Giovanelli, Martha P. Haynes, John J. Salzer, Gary Wegner

Dartmouth Scholarship

In preceding papers of this series, TF relations for galaxies in 24 clusters with radial velocities between 1000 and 9200 km s^-1 (SCI sample) were obtained, a Tully-Fisher (TF) template relation was constructed, and mean offsets of each cluster with respect to the template were obtained. Here, an estimate of the line-of-sight peculiar velocities of the clusters and their associated errors are given. It is found that cluster peculiar velocities in the cosmic microwave background reference frame do not exceed 600 km s^-1 and that their distribution has a line-of-sight dispersion of 300 km s^-1, suggesting …

Cluster Versus Field Elliptical Galaxies And Clues On Their Formation, Mariangela Bernardi, Alvio Renzini, Luiz N. Da Costa, Gary Wegner

Dartmouth Scholarship

Using new observations for a sample of 931 early-type galaxies, we investigate whether the Mg₂-σ₀ relation shows any dependence on the local environment. The galaxies have been assigned to three different environments depending on the local overdensity (clusters, groups, and field); we used our complete redshift database to guide the assignment of galaxies. It is found that cluster, group, and field early-type galaxies follow almost identical Mg₂-σ₀ relations, with the largest Mg₂ zero-point difference (clusters minus field) being only 0.007±0.002 mag. No correlation of the residuals is found with the morphological type or …

Conserved Elements Containing Nf-E2 And Tandem Gata Binding Sites Are Required For Erythroid-Specific Chromatin Structure Reorganization Within The Human Beta-Globin Locus Control Region, Oded Pomerantz, Andrew J. Goodwin, Terrence Joyce, Christopher H. Lowrey

Dartmouth Scholarship

Proper expression of the genes of the human β-globin gene locus requires the associated locus control region (LCR). Structurally, the LCR is defined by the presence of four domains of erythroid-specific chromatin structure. These domains, which have been characterized as DNase I hypersensitive sites (HSs), comprise the active elements of the LCR. The major focus of this research is to define the cis-acting elements which are required for the formation of these domains of unique chromatin structure. Our previous investigations on the formation of LCR HS4 demonstrated that NF-E2 and tandem, inverted GATA binding sites are required for the …

The Leukemic Protein Core Binding Factor Beta (Cbfbeta)-Smooth-Muscle Myosin Heavy Chain Sequesters Cbfalpha2 Into Cytoskeletal Filaments And Aggregates, Neeraj Adya, Terryl Stacy, Nancy A. Speck, Pu Paul Liu

Dartmouth Scholarship

The fusion gene CBFB-MYH11 is generated by the chromosome 16 inversion associated with acute myeloid leukemias. This gene encodes a chimeric protein involving the core binding factor β (CBFβ) and the smooth-muscle myosin heavy chain (SMMHC). Mouse model studies suggest that this chimeric protein CBFβ-SMMHC dominantly suppresses the function of CBF, a heterodimeric transcription factor composed of DNA binding subunits (CBFα1 to 3) and a non-DNA binding subunit (CBFβ). This dominant suppression results in the blockage of hematopoiesis in mice and presumably contributes to leukemogenesis. We used transient-transfection assays, in combination with immunofluorescence and green fluorescent protein-tagged proteins, to monitor …

Identification And Characterization Of Spcu, A Chaperone Required For Efficient Secretion Of The Exou Cytotoxin, Viviane Finck-Barbançon, Timothy L. Yahr, Dara W. Frank

Dartmouth Scholarship

In recent studies, we have shown that Pseudomonas aeruginosa strains that are acutely cytotoxic in vitro damage the lung epithelium in vivo. Genetic analysis indicated that the factor responsible for acute cytotoxicity was controlled by ExsA and therefore was part of the exoenzyme S regulon. The specific virulence determinant responsible for epithelial damage in vivo and cytotoxicity in vitro was subsequently mapped to the exoU locus. The present studies are focused on a genetic characterization of the exoU locus. Northern blot analyses and complementation experiments indicated that a region downstream of exoU was expressed and that the expression of this …

Strong Dissipative Behavior In Quantum Field Theory, Arjun Berera, Marcelo Gleiser, Rudnei O. Ramos

Dartmouth Scholarship

We study the conditions under which an overdamped regime can be attained in the dynamic evolution of a quantum field configuration. Using a real-time formulation of finite temperature field theory, we compute the effective evolution equation of a scalar field configuration, quadratically interacting with a given set of other scalar fields. We then show that, in the overdamped regime, the dissipative kernel in the field equation of motion is closely related to the shear viscosity coefficient, as computed in scalar field theory at finite temperature. The effective dynamics is equivalent to a time-dependent Ginzburg-Landau description of the approach to equilibrium …

Flows On Scales Of 150 Mpc?, K. R. Müller, W. Freudling, R. Watkins, G. Wegner

Dartmouth Scholarship

In order to investigate the reality of large-scale streaming motion on scales of up to 150 Mpc, we have studied the peculiar motions of ~200 early-type galaxies in three directions of the South Equatorial Strip at distances out to ~20,000 km s^-1. The new Automatic Plate Measuring Facility South Equatorial Strip Catalog (-175<δ<+25) was used to select the sample of field galaxies in three directions: (1) 15^h10^m-16^h10^m; (2) 20^h30^m-21^h50^m; (3) 00^h10^m-01^h30^m. New R-band CCD photometry and spectroscopic data for the galaxies are used. The fundamental plane distance-indicator …

The Seca Subunit Of Escherichia Coli Preprotein Translocase Is Exposed To The Periplasm, Jerry Eichler, William Wickner

Dartmouth Scholarship

SecA undergoes conformational changes during translocation, inserting domains into and across the membrane or enhancing the protease resistance of these domains. We now show that some SecA bound at SecYEG is accessible from the periplasm to a membrane-impermeant probe in cells with a permeabilized outer membrane but an intact plasma membrane.

Exoy, An Adenylate Cyclase Secreted By The Pseudomonas Aeruginosa Type Iii System, Timothy L. Yahr, Amy J. Vallis, Michael Hancock, Joseph T. Barbieri, Dara W. Frank

Dartmouth Scholarship

The exoenzyme S regulon is a set of coordinately regulated virulence genes of Pseudomonas aeruginosa. Proteins encoded by the regulon include a type III secretion and translocation apparatus, regulators of gene expression, and effector proteins. The effector proteins include two enzymes with ADP-ribosyltransferase activity (ExoS and ExoT) and an acute cytotoxin (ExoU). In this study, we identified ExoY as a fourth effector protein of the regulon. ExoY is homologous to the extracellular adenylate cyclases of Bordetella pertussis (CyaA) and Bacillus anthracis (EF). The homology among the three adenylate cyclases is limited to two short regions, one of which possesses an …

Transport Of Er Vesicles On Actin Filaments In Neurons By Myosin V, Joel S. Tabb, Bradley J. Molyneaux, Darien L. Cohen, Sergei A. Kuznetsov, George M. Langford

Dartmouth Scholarship

Axoplasmic organelles in the giant axon of the squid have been shown to move on both actin filaments and microtubules and to switch between actin filaments and microtubules during fast axonal transport. The objectives of this investigation were to identify the specific classes of axoplasmic organelles that move on actin filaments and the myosin motors involved. We developed a procedure to isolate endoplasmic reticulum (ER) from extruded axoplasm and to reconstitute its movement in vitro. The isolated ER vesicles moved on exogenous actin filaments adsorbed to coverslips in an ATP-dependent manner without the addition of soluble factors. Therefore myosin was …

Utility Driven Mobile-Agent Scheduling, Jonathan Bredin, David Kotz, Daniela Rus

Computer Science Technical Reports

Mobile agents are programs capable of migrating from one host machine to another. We propose that mobile agents purchase resource access rights from host machines thereby establishing a market for computational resources and giving agents a metric to evenly distribute themselves throughout the network. Market participation requires quantitative information about resource consumption to define demand and calculate utility. We create a formal utility model to derive user-demand functions, allowing agents to efficiently plan expenditure and deal with price fluctuations. By quantifying demand and utility, resource owners can precisely set a value for a good. We simulate our model in a …

Abstractions For Simplifying Planning In Self-Reconfigurable Robotic Systems, Craig Mcgray, Daniela Rus

Computer Science Technical Reports

In [KVRM], we described a three-dimensional self-reconfiguring robot module called the Molecule Robot. In this paper, we provide a system of abstractions for modules in self-reconfigurable robotic systems, and show how this system can be used to simplify the motion planning of the Molecule Robot system.

Hubble Space Telescope Images And Spectra Of The Remnant Of Sn 1885 In M31, Robert A. Fesen, Christopher L. Gerardy, Kevin M. Mclin, Andrew J. S. Hamilton

Dartmouth Scholarship

Near-UV Hubble Space Telescope images of the remnant of SN 1885 (S And) in M31 show a 070±005 diameter absorption disk silhouetted against M31's central bulge, at SN 1885's historically reported position. The disk's size corresponds to a linear diameter of 2.5±0.4 pc at a distance of 725±70 kpc, implying an average expansion velocity of 11,000±2000 km s-1 over 110 yr. Low-dispersion Faint Object Spectrograph spectra over 3200-4800 Å reveal that the absorption arises principally from Ca II H and K (equivalent width 215 Å), with weaker absorption features of Ca I 4227 Å and Fe I 3720 Å. The …

Peculiar Velocity Dipoles Of Field Galaxies, Riccardo Giovanelli, Martha P. Haynes, Wolfram Freudling, Luiz N. Da Costa, John J. Salzer, Gary Wegner

Dartmouth Scholarship

The Tully-Fisher (TF) relation is applied to obtain peculiar velocities of field spiral galaxies and to calculate dipoles of the peculiar velocity field to cz8000 km s^-1. The field galaxy sample is spatially coextensive with and completely independent of a cluster sample, for which dipole characteristics are given in a separate paper. Dipoles of the peculiar velocity field are obtained separately by applying (1) an inverse version of the TF relation and selecting galaxies by redshift windowing and (2) a direct TF relation, with velocities corrected for the inhomogeneous Malmquist bias and windowing galaxies by TF distance. …

Transport Of Axl2p Depends On Erv14p, An Er–Vesicle Protein Related To The Drosophila Cornichon Gene Product, Jacqueline Powers, Charles Barlowe

Dartmouth Scholarship

COPII-coated ER-derived transport vesicles from Saccharomyces cerevisiae contain a distinct set of membrane-bound polypeptides. One of these polypeptides, termed Erv14p (ER-vesicle protein of 14 kD), corresponds to an open reading frame on yeast chromosome VII that is predicted to encode an integral membrane protein and shares sequence identity with the Drosophila cornichon gene product. Experiments with an epitope-tagged version of Erv14p indicate that this protein localizes to the ER and is selectively packaged into COPII-coated vesicles. Haploid cells that lack Erv14p are viable but display a modest defect in bud site selection because a transmembrane secretory protein, Axl2p, is not …

Change In U.S. Tariffs: The Role Of Import Prices And Commercial Policies, Douglas A. Irwin

Dartmouth Scholarship

No abstract is available for this item.

Comparison Of The Sfi Peculiar Velocities With The Iras 1.2-Jy Gravity Field, L. N. Da Costa, A. Nusser, W. Freudling, R. Giovanelli, M. P. Haynes, J J. Salzer, G. Wegner

Dartmouth Scholarship

We present a comparison between the peculiar velocity fields measured from a recently completed I-band Tully-Fisher survey of field spirals (SFI) and that derived from the IRAS 1.2-Jy redshift survey galaxy distribution. The analysis is based on the expansion of these data in redshift space using smooth orthonormal functions, and is performed using low- and high-resolution expansions, with an effective smoothing scale which increases almost linearly with redshift. The effective smoothing scales at 3000 km s⁻¹ are 1500 and 1000 km s⁻¹ for the low- and high-resolution filters. The agreement between the high- and low-resolution SFI velocity …

Developmental Regulation Of A Cyclin-Dependent Kinase Inhibitor Controls Postembryonic Cell Cycle Progression In Caenorhabditis Elegans, Yang Hong, Richard Roy, Victor Ambros

Dartmouth Scholarship

C. elegans cki-1 encodes a member of the CIP/KIP family of cyclin-dependent kinase inhibitors, and functions to link postembryonic developmental programs to cell cycle progression. The expression pattern of cki-1::GFP suggests that cki-1 is developmentally regulated in blast cells coincident with G1, and in differentiating cells. Ectopic expression of CKI-1 can prematurely arrest cells in G1, while reducing cki-1 activity by RNA-mediated interference (RNAi) causes extra larval cell divisions, suggesting a role for cki-1 in the developmental control of

INTRODUCTION

The proper development of a multicellular organism requires the precise orchestration of cell proliferation and differentiation. Despite considerable progress toward …

Applications Of Parallel I/O, Ron Oldfield, David Kotz

Computer Science Technical Reports

Scientific applications are increasingly being implemented on massively parallel supercomputers. Many of these applications have intense I/O demands, as well as massive computational requirements. This paper is essentially an annotated bibliography of papers and other sources of information about scientific applications using parallel I/O. It will be updated periodically.

Hot White Dwarfs In The Extreme ‐ Ultraviolet Explorer Survey. Iv. Da White Dwarfs With Bright Companions, Stephane Vennes, Damian J. Christian, John R. Thorstensen

Dartmouth Scholarship

We present an analysis of optical, ultraviolet, and X-ray spectral properties of a sample of 13 hot hydrogen-rich (DA) white dwarfs, each paired with a luminous unresolved companion. Using low-dispersion International Ultraviolet Explorer spectra, ROSAT photometry, and Extreme-Ultraviolet Explorer photometry and spectroscopy, we estimate the effective temperature, mass, and distance of the white dwarfs. Additionally, we examine the question of their atmospheric composition. We establish orbital properties for most binaries by means of high-dispersion optical spectroscopy obtained with the Hamilton echelle spectrograph at Lick Observatory; the same data help uncover evidence of activity in some of the secondary …

Cd4(+) T Cells Tolerized Ex Vivo To Host Alloantigen By Anti-Cd40 Ligand (Cd40l:Cd154) Antibody Lose Their Graft-Versus-Host Disease Lethality Capacity But Retain Nominal Antigen Responses., Bruce R. Blazar, Patricia A. Taylor, Randolph J. Noelle, Daniel A. Vallera

Dartmouth Scholarship

A major goal of the transplant field is to tolerize donor T cells to prevent graft-versus-host disease (GVHD) (1). We describe an ex vivo approach in which the blockade of CD40 ligand (CD40L:CD154):CD40 interactions, a pathway required for optimal T cell expansion, induces donor CD4(+) T cells to become tolerant to host alloantigens (2). High doses of tolerized cells did not cause GVHD lethality in vivo. T cells had intact responses to antigens not present during tolerization. Tolerance was long lived and not readily reversible in vivo. These data have significant implications for the use of tolerization approaches to prevent …

Molecular Basis For Effects Of Carcinogenic Heavy Metals On Inducible Gene Expression, Joshua W. Hamilton, Ronald C. Kaltreider, Olga V. Bajenova, Michael A. Ihnat, Jennifer Mccaffrey, Bruce W. Turpie, Erin E. Rowell, Jannet Oh, Michael J. Nemeth, Carrie A. Pesce, Jean P. Lariviere

Dartmouth Scholarship

Certain forms of the heavy metals arsenic and chromium are considered human carcinogens, although they are believed to act through very different mechanisms. Chromium(VI) is believed to act as a classic and mutagenic agent, and DNA/chromatin appears to be the principal target for its effects. In contrast, arsenic(III) is considered nongenotoxic, but is able to target specific cellular proteins, principally through sulfhydryl interactions. We had previously shown that various genotoxic chemical carcinogens, including chromium (VI), preferentially altered expression of several inducible genes but had little or no effect on constitutive gene expression. We were therefore interested in whether these carcinogenic …

Design Of An Epidemiologic Study Of Drinking Water Arsenic Exposure And Skin And Bladder Cancer Risk In A U.S. Population, Margaret R. Karagas, Tor D. Tosteson, Joel Blum, J Steven Morris, John A. Baron, Bjoern Klaue

Dartmouth Scholarship

Ingestion of arsenic-contaminated drinking water is associated with an increased risk of several cancers, including skin and bladder malignancies; but it is not yet clear whether such adverse effects are present at levels to which the U.S. population is exposed. In New Hampshire, detectable levels of arsenic have been reported in drinking water supplies throughout the state. Therefore, we have begun a population-based epidemiologic case-control study in which residents of New Hampshire diagnosed with primary squamous cell (n = 900) and basal cell (n = 1200) skin cancers are being selected from a special statewide skin cancer incidence survey; patients …

Expression Of Toxoplasma Gondii-Specific Heat Shock Protein 70 During In Vivo Conversion Of Bradyzoites To Tachyzoites, Neide M. Silva, Ricardo T. Gazzinelli, Deise A. O. Silva, Eloisa A. V. Ferro, Lloyd H. Kasper, Jose R. Mineo

Dartmouth Scholarship

Stage conversion between bradyzoites and tachyzoites was investigated in C57BL/6 mice chronically infected with the ME-49 strain of Toxoplasma gondii. In order to promote bradyzoite-tachyzoite conversion, mice were treated in vivo with neutralizing doses of anti-gamma interferon (IFN-gamma) or anti-tumor necrosis factor alpha (TNF-alpha) antibodies. Expression of parasite-specific antigens SAG-1, SAG-2, and heat shock protein 70 (Hsp-70) was visualized in the central nervous system by immunocytochemistry and measured by photometric assay. The immunosuppressive effect of anti-IFN-gamma or anti-TNF-alpha treatment was immediate, leading to parasite stage conversion as indicated by the increased expression of tachyzoite-specific antigens (SAG-1 and SAG-2) and by …

Hormone Replacement Therapy And Risk Of Hip Fracture: Population Based Case-Control Study, Karl Michaëlsson, John A. Baron, Bahman Y. Farahmand, Olof Johnell, Cecilia Magnusson, Per-Gunnar Persson, Ingemar Persson, Sverker Ljunghall

Dartmouth Scholarship

Objective: To determine the relative risk of hip fracture associated with postmenopausal hormone replacement therapy including the effect of duration and recency of treatment, the addition of progestins, route of administration, and dose. Design: Population based case-control study. Setting: Six counties in Sweden. Subjects: 1327 women aged 50-81 years with hip fracture and 3262 randomly selected controls. Main outcome measure: Use of hormone replacement therapy. Results: Compared with women who had never used hormone replacement therapy, current users had an odds ratio of 0.35 (95% confidence interval 0.24 to 0.53) for hip fracture and former users had an odds ratio …

A V And I Ccd Mosaic Survey Of The Ursa Minor Dwarf Spheroidal Galaxy, J. T. Kleyna, M. J. Geller, S. J. Kenyon, M. J. Kurtz, J. R. Thorstensen

Dartmouth Scholarship

We discuss a Johnson-Cousins V- and I-band CCD mosaic survey of the Ursa Minor dwarf spheroidal galaxy (dSph) to V ~ 22. We covered UMi with 27 105 × 105 overlapping CCD frames, each frame consisting of two 300 s exposures in each of V and I. We also observed several regions ~3° from UMi to obtain an estimate of contamination by galaxies and Galactic stars. We report the first H-R diagram of an entire dSph. Separation of dwarf stars from foreground stars by color allows a robust estimation of the structural parameters of UMi. We examine …