Open Access. Powered by Scholars. Published by Universities.®
- Publication
Articles 1 - 2 of 2
Full-Text Articles in Entire DC Network
The Role Of Broad In Determining Neuronal Composition In The Drosophila Brain, Alper Dincer
The Role Of Broad In Determining Neuronal Composition In The Drosophila Brain, Alper Dincer
Honors Theses
To elucidate the individual roles of the four Broad-Complex (BR-C) isoforms, Z1-Z4, on neuronal composition in the mushroom body, I undertook a series of overexpression experiments and created tools for knockdown experiments. Specifically, I imaged and analyzed Drosophila brains from earlier experiments in which BR-C isoforms Z1 and Z3 were individually overexpressed in the MB. The knockdown experiments required the creation of the molecular tools necessary for isoform-specific RNA interference (RNAi). For these I performed PCR to amplify DNA sequences unique to each isoform and inserted those into the pWIZ vector, which will permit expression of loopless hairpin double stranded …
The Overexpression Of Homeotic Complex Gene Ultrabithorax In The Post-Embryonic Neuronal Lineages Of The Ventral Nervous System In Drosophila Melanogaster, Katie Dry
Master’s Theses
This study uses a molecular technique called MARCM (Mosaic Analysis with a Repressible Cell Marker) to label neuronal lineages that overexpress the Hox gene Ultrabithorax (Ubx) in an unlabeled, wild type background. The results indicate that the overexpression of Ubx is sufficient to transform more anterior neuronal lineages to themorphology of their more posterior counterparts. The data presented here begin to elucidate the role that the Hox genes have in shaping segment-specific neural connections in the post-embryonic ventral nervous system.