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Environmental Issues In Russia, Laura A. Henry, Vladimir Douhovnikoff
Environmental Issues In Russia, Laura A. Henry, Vladimir Douhovnikoff
Biology Faculty Publications
This review examines the literature available on the state of the environment and environmental protection in the Russian Federation. As the largest country on Earth, rich in natural resources and biodiversity, Russia's problems and policies have global consequences. Environmental quality and management are influenced by the legacy of Soviet economic planning and authoritarian governance, as well as by Russia's post-Soviet economic recession and current strategies of economic development. Russia achieved a reduction in some pollutants owing to the collapse of industrial production in the 1990s, but many environmental indicators suggest growing degradation. Russia has signed on to a number of …
A Simple Polymerase Chain Reaction-Based Method For The Construction Of Recombinase-Mediated Cassette Exchange Donor Vectors, Jack R. Bateman, C. Ting Wu
A Simple Polymerase Chain Reaction-Based Method For The Construction Of Recombinase-Mediated Cassette Exchange Donor Vectors, Jack R. Bateman, C. Ting Wu
Biology Faculty Publications
Here we describe a simple method for generating donor vectors suitable for targeted transgenesis via recombinase-mediated cassette exchange (RMCE) using the ΦC31 integrase. This PCR-based strategy employs small attB "tails" on the primers used to amplify a sequence of interest, permitting the rapid creation of transgenes for in vivo analysis. Copyright © 2008 by the Genetics Society of America.
A Genomewide Survey Argues That Every Zygotic Gene Product Is Dispensable For The Initiation Of Somatic Homolog Pairing In Drosophila, Jack R. Bateman, C. Ting Wu
A Genomewide Survey Argues That Every Zygotic Gene Product Is Dispensable For The Initiation Of Somatic Homolog Pairing In Drosophila, Jack R. Bateman, C. Ting Wu
Biology Faculty Publications
Studies from diverse organisms show that distinct interchromosomal interactions are associated with many developmental events. Despite recent advances in uncovering such phenomena, our understanding of how interchromosomal interactions are initiated and regulated is incomplete. During the maternal-to-zygotic transition (MZT) of Drosophila embryogenesis, stable interchromosomal contacts form between maternal and paternal homologous chromosomes, a phenomenon known as somatic homolog pairing. To better understand the events that initiate pairing, we performed a genomewide assessment of the zygotic contribution to this process. Specifically, we took advantage of the segregational properties of compound chromosomes to generate embryos lacking entire chromosome arms and, thus, all …
Molecular Basis Of The Copulatory Plug Polymorphism In Caenorhabditis Elegans, Michael F. Palopoli, Matthew V. Rockman, Aye Tinmaung, Camden Ramsay, Stephen Curwen, Andrea Aduna, Jason Laurita, Leonid Kruglyak
Molecular Basis Of The Copulatory Plug Polymorphism In Caenorhabditis Elegans, Michael F. Palopoli, Matthew V. Rockman, Aye Tinmaung, Camden Ramsay, Stephen Curwen, Andrea Aduna, Jason Laurita, Leonid Kruglyak
Biology Faculty Publications
Heritable variation is the raw material for evolutionary change, and understanding its genetic basis is one of the central problems in modern biology. We investigated the genetic basis of a classic phenotypic dimorphism in the nematode Caenorhabditis elegans. Males from many natural isolates deposit a copulatory plug after mating, whereas males from other natural isolates-including the standard wild-type strain (N2 Bristol) that is used in most research laboratories-do not deposit plugs. The copulatory plug is a gelatinous mass that covers the hermaphrodite vulva, and its deposition decreases the mating success of subsequent males. We show that the plugging polymorphism results …
Pheromones Enhance Somatosensory Processing In Newt Brains Through A Vasotocin-Dependent Mechanism, R. R. Thompson, P. S. Dickinson, J. D. Rose, K. A. Dakin, G. M. Civiello, A. Segerdahl, R. Bartlett
Pheromones Enhance Somatosensory Processing In Newt Brains Through A Vasotocin-Dependent Mechanism, R. R. Thompson, P. S. Dickinson, J. D. Rose, K. A. Dakin, G. M. Civiello, A. Segerdahl, R. Bartlett
Biology Faculty Publications
We tested whether the sex pheromones that stimulate courtship clasping in male roughskin newts do so, at least in part, by amplifying the somatosensory signals that directly trigger the motor pattern associated with clasping and, if so, whether that amplification is dependent on endogenous vasotocin (VT). Female olfactory stimuli increased the number of action potentials recorded in the medulla of males in response to tactile stimulation of the cloaca, which triggers the clasp motor reflex, as well as to tactile stimulation of the snout and hindlimb. That enhancement was blocked by exposing the medulla to a V1a receptor antagonist before …
The Pyloric Neural Circuit Of The Herbivorous Crab Pugettia Producta Shows Limited Sensitivity To Several Neuromodulators That Elicit Robust Effects In More Opportunistically Feeding Decapods, Patsy S. Dickinson, Elizabeth A. Stemmler, Andrew E. Christie
The Pyloric Neural Circuit Of The Herbivorous Crab Pugettia Producta Shows Limited Sensitivity To Several Neuromodulators That Elicit Robust Effects In More Opportunistically Feeding Decapods, Patsy S. Dickinson, Elizabeth A. Stemmler, Andrew E. Christie
Biology Faculty Publications
Modulation of neural circuits in the crustacean stomatogastric nervous system (STNS) allows flexibility in the movements of the foregut musculature. The extensive repertoire of such resulting motor patterns in dietary generalists is hypothesized to permit these animals to process varied foods. The foregut and STNS of Pugettia producta are similar to those of other decapods, but its diet is more uniform, consisting primarily of kelp. We investigated the distribution of highly conserved neuromodulators in the stomatogastric ganglion (STG) and neuroendocrine organs of Pugettia, and documented their effects on its pyloric rhythm. Using immunohistochemistry, we found that the distributions of Cancer …
Probing The Mechanism Of Recognition Of Ssdna By The Cdc13-Dbd, Aimee M. Eldridge, Deborah S. Wuttke
Probing The Mechanism Of Recognition Of Ssdna By The Cdc13-Dbd, Aimee M. Eldridge, Deborah S. Wuttke
Biology Faculty Publications
The Saccharomyces cerevisiae protein Cdc13 tightly and specifically binds the conserved G-rich single-stranded overhang at telomeres and plays an essential role in telomere end-protection and length regulation. The 200 residue DNA-binding domain of Cdc13 (Cdc13-DBD) binds an 11mer single-stranded representative of the yeast telomeric sequence [Tel11, d(GTGTGGGTGTG)] with a 3 pM affinity and specificity for three bases (underlined) at the 5′ end. The structure of the Cdc13-DBD bound to Tel11 revealed a large, predominantly aromatic protein interface with several unusual features. The DNA adopts an irregular, extended structure, and the binding interface includes a long (∼30 amino acids) structured loop …