Open Access. Powered by Scholars. Published by Universities.®
Articles 1 - 2 of 2
Full-Text Articles in Entire DC Network
Accelerating Variety Release With Double Haploids, Sue Broughton
Accelerating Variety Release With Double Haploids, Sue Broughton
Journal of the Department of Agriculture, Western Australia, Series 4
The use of plant tissue culture to produce special lines called doubled haploids is reducing the time taken to breed new varieties of cereal crops by up to three years. Sue Broughton outlines what doubled haploids are, how they are produced, and why they have been able to short circuit the usual lengthy breeding process.
Marker Gene Delivery To Mature Wheat Embryos Via Particle Bombardment, Hüseyi̇n Avni̇ Öktem, Füsun İnci̇ Eyi̇doğan, Fahri̇ye Setenci̇ Ertuğrul, Meral Yücel, Barnabas Jenes, Otto Toldi
Marker Gene Delivery To Mature Wheat Embryos Via Particle Bombardment, Hüseyi̇n Avni̇ Öktem, Füsun İnci̇ Eyi̇doğan, Fahri̇ye Setenci̇ Ertuğrul, Meral Yücel, Barnabas Jenes, Otto Toldi
Turkish Journal of Botany
The possibility of transferring genes to mature wheat embryos ( Triticum aestivumL. and T. durum Desf.) via accelerated and DNA-coated tungsten particles was investigated. Mature embryos isolated from bread (cv. Atay) and durum (cv. Çakmak) wheat were utilised as targets for bombardment. DNA in the form of circular plasmid (pBSGUSINT) was precipitated on tungsten particles (ca. 2 \mum diameter) using the calcium nitrate method. Mature embryos were bombarded by a microprocessor-controlled particle delivery instrument (GENEBOOSTER TM ) driven by compressed nitrogen gas. Bombardment was carried out at various gas pressure and in a chamber vacuum. The target material was subjected …