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Presence Of A Cs-Resistant Transient Outward Current Which Distorts Ica Measurements In Guinea-Pig Single Ventricular Cells, Georges Christé, Brigitte Delachapelle, Fouad Lemtiri-Chlieh, Carlos Ojeda
Presence Of A Cs-Resistant Transient Outward Current Which Distorts Ica Measurements In Guinea-Pig Single Ventricular Cells, Georges Christé, Brigitte Delachapelle, Fouad Lemtiri-Chlieh, Carlos Ojeda
fouad Lemtiri-Chlieh
To correctlyr analyse the physical properties of ion channels and the actions of drugs upon them, it is important that the membrane conducts only the ionic species believed permeant. In guinea-pig ventricular cells it has been assumed that only ICaL flows in the potential range -50 to +60 mV in the presence of intra- and extracellular Cs (> 20 mM). We report here that this is not the case due to the presence of a Cs-insensitive, transient outward current.
Measurements Of Cytosolic Free Ca In Cultured Rat Neonate And Adult Guinea-Pig Ventricular Cardiac Myocytes, Jean-Claude Bernengo, Fouad Lemtiri-Chlieh, Carlos Ojeda, Nadine Pltonoff
Measurements Of Cytosolic Free Ca In Cultured Rat Neonate And Adult Guinea-Pig Ventricular Cardiac Myocytes, Jean-Claude Bernengo, Fouad Lemtiri-Chlieh, Carlos Ojeda, Nadine Pltonoff
fouad Lemtiri-Chlieh
Cytosolic free Ca was measured with Indo-1 (Grynkiewicz et al. 1985) as free acid (in guinea-pig cells) or as the ester (in rat neonate cells). Using image analysis we obtained concentration maps at 40 ms intervals at two wavelengths (Cannell et al. 1986). In rat neonate cells, at rest and during contraction, calcium was apparently non-uniformly distributed, in contrast to guinea-pig adult cells. Using a dual PM spectroscopic system adapted to an inverted microscope we found: (1) the conversion of Indo-AM to Indo- 1 in the cytosol is not complete (< 40% contribution to the total light); (2) rat neonate cells buffer Ca (at rest 130-170 nM) only for [Ca]. between 1 and 3 mM, whereas in adult guinea-pig cells the range extended to 10 mM; (3) during contraction [Ca]i rose from 200 nm to no more than 1 microM. Digitoxin increases basal and peak [Ca]i; (4) during spontaneous contractions, in guinea-pig cells, [Ca]i rises from 70 to 200 nm when [Ca]o = 0, and from 170 nm to about 1 microM when [Ca]o = 2 mM; (5) in voltage-clamp conditions the rise in [Ca]i does not exceed 2 microM and oscillations occur in the presence of forskolin. When loading with Indo-1, cells frequently cease to contract (Powell et al. 1988) and the variations in [Ca]i are then very small (about 200 nm at peak ICa). In both cases these values are small compared to the total charge crossing the membrane.
Phosphorus Fertilization Through Drip Irrigation, Robert Mikkelsen
Phosphorus Fertilization Through Drip Irrigation, Robert Mikkelsen
Robert Mikkelsen
Although P fertilization through drip irrigation systems has not been widely recommended, a review of currently available literature indicates this fertilizer application technique can offer many advantages when performed properly. Using drip irrigation to apply P fertilizer allows nutrient placement directly into the plant root zone during critical periods of nutrient demand. Less P fertilizer is generally required to achieve sufficient tissue P concentrations and equivalent yields when it is drip-applied than with other application methods. The distribution of drip-applied P in soil depends on soil properties, the source of P fertilizer, the rate of application, and the amount of …
Factors Affecting Selenium Accumulation By Agricultural Crops, Robert Mikkelsen, Albert L. Page, Frank T. Bingham
Factors Affecting Selenium Accumulation By Agricultural Crops, Robert Mikkelsen, Albert L. Page, Frank T. Bingham
Robert Mikkelsen
The accumulation of selenium by plants is of concern worldwide. Many regions grow crops that contain insufficient Se to meet animal nutritional requirements. In these locations with low Se, efforts have been made to increase tissue Se concentrations. Other areas have problems with excessive Se found in vegetation grown on seleniferous soils. Plant species vary in their ability to accumulate and tolerate Se. Selenium accumulator plants can accumulate extremely high Se concentrations (several thousand mg Se kg-1) when grown in seleniferous soils, whereas typical agricultural crops have a much lower Se tolerance (<50 mg Se kg>-1 ). It has been suggested that …