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Identification And Characterization Of Isoflavone Reductase Family Members In Soybean, Negin Azizkhani

Electronic Thesis and Dissertation Repository

Soybean’s yield is threatened by Phytophthora sojae, a pathogen responsible for stem and root rot disease. Glyceollins, unique antimicrobial agents specific to soybeans in partially preventing P. sojae infection, are derived from the isoflavonoid branch of the general phenylpropanoid pathway. One pivotal enzyme exclusively involved in glyceollin synthesis in soybean is the isoflavone reductase (GmIFR), which catalyzes the 2'-hydroxydaidzein conversion to 2'-hydroxy-2,3-dihydrodaidzein as a precursor for glyceollin biosynthesis. To comprehensively identify all members of the GmIFR gene family within the soybean genome, keyword and blast protein searches were conducted, identifying 98 putative GmIFRs. Among these candidates, …

A Robust Agroinfiltration Method, Bryce N. Trull

Masters Theses

Stable transformation of soybean (Glycine max) is a markedly slow and laborious process. Thus, a tool that enables rapid evaluation of genetic elements in planta is critical to advance complex research and genetic engineering in soybean. To that end, a substantially robust agroinfiltration method was innovated in this work. Agroinfiltration is a technique that leverages Agrobacterium-mediated transient transformation to deliver genetic elements to the cells of whole plant tissues, usually leaves. Several factors were found to be relevant to successful soybean leaf agroinfiltration, including genotype, surfactant, developmental stage, and Agrobacterium culture medium. The research represents not only …

Evaluation Of Weed Control Efficacy And Crop Tolerance To Florpyrauxifen-Benzyl Coated Onto Urea, Bodie Lyn Cotter

Graduate Theses and Dissertations

Frequent off-target movement occurrences to soybean [Glycine max (L.) Merr.], rice (Oryza sativa L.) injury, and a lack of barnyardgrass [Echinochloa crus-galli (L.) P. Beauv.] control followed the commercial launch of florpyrauxifen-benzyl in 2018. Florpyrauxifen-benzyl is a synthetic auxin, Herbicide Resistance Action Committee (HRAC)/Weed Science Society of America (WSSA) Group 4 herbicide used for postemergence weed control in rice and labeled for application at 30 g ae ha-1. Previous research documented that soybean was susceptible to injury following spray applications of florpyrauxifen-benzyl at less-than-labeled rates, and certain varieties of rice are less tolerant to spray-applied florpyrauxifen-benzyl at 30 g ae …

Survey And Prevalence Of Palmer Amaranth Herbicide Resistance In South Carolina, Mitchell Williams

All Theses

Palmer amaranth is a troublesome weed for growers to control, not only due to its aggressive growth characteristics that limit row-crop production, but because of its resistance to different herbicide modes of action. The first case of herbicide resistance in Palmer amaranth was detected in 1989 and has since grown to nine different herbicide classes throughout the United States. New herbicide modes of action have not been developed since the 1980s, so proper stewardship of the remaining modes of action is important for effective control of Palmer amaranth. Increased herbicide resistance from states bordering South Carolina have been reported; therefore, …

Precision Management Of Inputs In Cotton And Soybean Production In South Carolina, Kyle Smith

All Theses

The adoption of precision agriculture technologies and developing specific product use recommendations in cotton and soybean production could help farmers reduce input costs and optimize overall farm profitability. The objectives of this research were to evaluate whether or not the use of variable rate seeding in cotton could increase profitability and to determine the rainfast interval of commonly used insecticides in cotton and soybean production. The first trial, variable rate seeding in cotton, was implemented at the Edisto Research and Education Center near Blackville, SC across five years to evaluate variable rate seeding in cotton. Results from trials in South …

Effect Od Different Sources And Application Rates Of Sulfur On Corn And Soybean Production Systems In Louisiana, Diego Mayorga Valladares

LSU Master's Theses

Sulfur (S) is a structural component of amino acids such as cysteine and methionine and is involved in important functions within the plant like photosynthesis, carbon and nitrogen metabolism, protein synthesis of oils, and detoxification mechanisms. Sulfur deficiency in crops has intensified around the world. Some of the reasons are improvement in controlling SO₂ emissions from industries, growing usage of high analysis S-free fertilizers and augmented cropping intensity. Sulfur fertilization has become an important factor in crop production systems and fertilization guidelines for S need to be up-to-date to improve use efficiency and compensate for rising prices of fertilizers. …

Metagenomic Analysis Of Root-Associated Microbiome Of Healthy And Taproot Decline-Affected Soybeans And Identification Of Healthy Soybean Root Endophytes With Protective Activity Against The Causal Agent, Xylaria Necrophora, Uyen Wesser

Theses and Dissertations

Plant roots are associated with a diverse microbial community of bacteria and fungi. The root microbiome communities associate with the root surface or penetrate the superficial layers of root tissues. It is of high agronomic interest to characterize root microbiomes and identify microbes with beneficial effects on plant defense against pathogens. Soybean taproot decline (TRD) is an emerging soil-borne disease caused by a fungus classified as Xylaria necrophora, challenging to control and with devastating effects on yield. Our group has initiated a study of the soybean root microbiome with three primary experiments: (I) perform a comparative study of root …

Characterization And Management Of Auxin-Resistant Palmer Amaranth In Tennessee, Delaney C. Foster

Doctoral Dissertations

Palmer amaranth has a long history of evolving resistance to herbicides to the point it has become a significant row crop production obstacle. Cotton and soybean growers were offered new technologies in 2016, expanding in-crop herbicide options to include dicamba or 2,4-D. Within three years of commercialization, dicamba use in these crops increased ten-fold and growers began to report Palmer amaranth escapes in west Tennessee auxin-tolerant production systems. A survey of Palmer amaranth escapes in dicamba and 2,4-D-tolerant cotton and soybean fields in Tennessee was conducted in the fall of 2021 with the objective of determining if poor control was …

Functional Analysis Of Soybean Proteinase Inhibitor Genes And Cyst Nematode-Inducible Synthetic Promoters For Insects And Nematode-Resistance In Plants, Mst Shamira Sultana

Doctoral Dissertations

Proteinase inhibitors (PIs) from legumes have the potential for use as protectants in response to pests and pathogens. Soybean (Glycine max) contains two trypsin inhibitors (TIs): Kunitz trypsin inhibitor (KTI) and Bowman-Birk inhibitor (BBI). In this study, the possible role of soybean TIs in plant defense against insects and nematodes was investigated. In addition to the three known TIs (KTI1, KTI2 and KTI3), novel inhibitors KTI5, KTI7, and BBI5 were identified in soybean. Their functional role was further examined by overexpression in soybean and Arabidopsis. In vitro enzyme inhibitory assays showed significant increase in trypsin and chymotrypsin inhibitory …

Breeding Soybean [Glycine Max (L) Merr.] Under Reduced Irrigation, Francia Seconde Ravelombola

Graduate Theses and Dissertations

Soybean [Glycine max (L) Merr.], a legume species native to East Asia in the Fabaceae family, ranks among the most important food crops in the world. It is widely grown and known for its high protein and oil concentration. Soybean is valuable because its seeds have multiple applications in food, feed, pharmaceutical, and industrial enterprises. Even though seed yield is the most important trait, breeders have recently given a significant attention to quality traits, such as high protein or modified oil concentration. Soybean seed protein inheritance has been extensively studied; however, genetics of high-protein ‘BARC-7’ soybean are still unknown.On the …

Root Phenotyping Of Peptide-Treated Glycine Max, Salem Jackson

Biological Sciences Undergraduate Honors Theses

Plant elicitor peptides (Peps) – endogenous chains of amino acids involved in natural plant defense – have been shown to decrease damage from herbivores and pathogens by inducing an immune response, increasing the emission of volatile organic compounds (VOCS), transcripts, and metabolites. Exogenous treatment of soybean seeds with plant elicitor peptide GmPep3 has been shown to induce these broad-spectrum defenses and offers a new method for increasing crop yield. However, the effects of GmPep3 on indicators of soybean health – root characteristics, growth stages, etc. – have not been fully realized.

Using the root-phenotyping platform RhizoVision Explorer, several root traits …

Re-Evaluation Of Late Season Action Thresholds And The Effect Of Seed Treatments On Early Season Colonization Of Threecornered Alfalfa Hopper, Spissistilus Festinus (Say), In Louisiana Soybean, Glycine Max (L) Merr, Tyler Ray Tagle Musgrove

LSU Master's Theses

Soybean, Glycine max (L.) Merr, is considered the most economically important oil and feed crop in the US and is grown on nearly 35,000,000 ha on an annual basis. Factors that limit yield include weed pressure, disease infection, and of particular interest to entomologists, insect herbivory. Soybean is targeted by a range of above and below ground pests which are primarily controlled by insecticides. Neonicotinoids are a recently popularized insecticide class that when applied as a seed treatment, can provide protection from early season pests. The most economically significant early season pest is threecornered alfalfa hopper, Spissistilus festinus (Say), whose …

Impact Of New Technologies On Weed Control In Louisiana Rice Production, David C. Walker

LSU Doctoral Dissertations

Field studies were conducted in 2018-2021 at the LSU Agricultural Center’s H.R. Caffey Rice Research Station (RRS) near Crowley, LA, and the Dean Lee Research and Extension Center (DLREC) near Alexandria, LA to evaluate off-target florpyrauxifen movement on soybean. Soybean was treated with florpyrauxifen at the V4-V5 or R1-R2 growth stages. Soybean injury was evaluated at 1, 7, 14, and 28 days after treatment (DAT) and soybean plant height (cm) was recorded at 7, 14, and 28 DAT. At soybean maturity, yield and individual yield components were subjected to regression analysis to describe the relationship between florpyrauxifen rate and soybean …

Design Of Affordable Polyaniline - Based Wearable Sensor For Real Time Plant Growth Monitoring, Temitope Borode

Electronic Theses and Dissertations

The need for precision agriculture in today and future farming cannot be overstressed. Recent agriculture is characterized by numerous challenges such as environmental stressors and resource constraints resulting in low crop yield and unsustainable agriculture practices. Understanding plant growth mechanism and growth rate under the external influence is a bedrock towards precision agriculture practices ensuring optimum yield and sustainable utilization of resources. The current study addresses this need by developing and applying an affordable and stable sensor for monitoring plant growth. The sensor was fabricated by in-situ chemical polymerization of aniline on an elastic band substrate by dip coating. The …

Relative Soybean Response To Aminocyclopyrachlor, 2,4-D, Dicamba, And Aminopyralid, Trey I. Clark

Masters Theses

This research compared the visual symptomology of four auxin herbicides applied at three rates to simulate tank contamination on non-auxin tolerant soybeans. The herbicides examined were aminocyclopyrachlor (ACP), 2,4-D, dicamba, and aminopyralid. The standard labeled rates (SLR) used to base the application rates were 0.12 kg ae ha^-1 for ACP and aminopyralid while dicamba and 2,4-D used 1.12 kg ae ha^-1. These four herbicides exhibit similar symptoms on soybeans which is problematic when diagnosing soybean response. One goal of this project was to obtain high-quality images of the four herbicide’s effect on soybeans, with the hope of …

Influence Of Planting Date, Maturity Group, Harvest Aids And Fungicide Application On Soybean (Glycine Max (L.) Seed Quality, Ana Priscila Campos

LSU Master's Theses

Field studies evaluated the influence of planting date, maturity group, and harvest timing on soybean yield and seed quality at the LSU AgCenter Northeast (NERS), Macon Ridge, and Dean Lee Research Stations in 2018-2020. In addition, the influence of harvest aid and fungicide application on seed quality impact from delayed harvest and environment was investigated at NERS in 2019 and 2020 in both field and environmentally controlled growth chambers. Soybean yield was maximized when maturity group IV and V soybean varieties were planted between Mid-April and Mid-May. Earlier and later planting dates did not result in maximized yield. Planting date …

Fungicide Sensitivity Screening Of Corynespora Cassiicola In Us Soybean And Cotton, Tyler Miles Smith

Doctoral Dissertations

Target spot, caused by the fungus Corynespora cassiicola, is a foliar disease of cotton and soybean. Target spot has become a disease of concern in soybean and cotton production systems. Data for fungicide sensitivity and understanding potential impact on yield is lacking for C. cassiicola. Fungicide resistance is also a concern in C. cassiicola with the increase in resistant pathogens in soybean. With the lack of knowledge about baseline fungicide sensitivity and the concern of fungicide resistance the objectives of this study were: (i) to evaluate fungicide products and mixes in small plot soybean and cotton trials, (ii) …

Screening And Breeding Soybean For Flood Tolerance, Maria Roberta De Oliveira

Graduate Theses and Dissertations

Waterlogging can be detrimental to soybean [Glycine max (L.) Merr.] growth and development, with effects ranging from chlorosis and stunting to yield loss and plant death. Soybean responses to, and the effects of, waterlogging are dependent on the growth stage of the plant at the initiation of waterlogging. The objectives of this study were: (1) to assess the effectiveness of Genomic Selection (GS), Marker Assisted Selection (MAS) and Phenotypic Selection for flood tolerance at the progeny row stage as compared to random selection, for the development of high-yielding flood-tolerant lines; and (2) to compare field-screening and hydroponic greenhouse screening methodologies …

Identifying Drought Tolerance Traits In Tennessee Soybean Genotypes: Recovery From Severe Water Deficit Stress, Samuel W. Purdom

Masters Theses

Ninety-four percent of U.S. grown soybeans are produced under rainfed conditions with intermittent droughts occurring late in the season during reproductive growth stages. Due to the temporary nature of drought, the ability of a crop to survive and recover effectively from water deficit stress is important for ensuring yield stability. In 2019 and 2020, two greenhouse experiments and two field studies were conducted to screen eleven soybean genotypes for transpiration response and recovery from water deficit stress and high vapor pressure deficit (VPD). In the first greenhouse study, soybean plants were grown in pots sealed to prevent evaporation and plants …

The Role Of The Auxin Biosynthesis And Signaling In Soybean Root Nodule Development, Mucahid Bozkus

Electronic Theses and Dissertations

Nitrogen(N) is one of the most important plant nutrients for plant growth and yield, however, its abundance in the soil is not sufficient for profitable crop production. The use of chemical fertilizers helps address soil N deficiency in agriculture. However, due to the environmental pollution resulting from excessive use of fertilizers, alternative forms of N for agriculture are a necessity. Leguminous plants such as soybean (Glycine max) form a symbiotic association with N-fixing rhizobia to meet their N demands. Legume-rhizobia symbiosis results in the formation of unique structures called nodules where rhizobia convert atmospheric nitrogen into plant usable forms, thus …

Crop Response To Low-Dose Dicamba, Mason Castner

Graduate Theses and Dissertations

The introduction of dicamba-resistant (DR) soybean [Glycine max (L.) Merr.] and cotton (Gossypium hirsutum L.) followed by a label allowing over-the-top applications of dicamba in the 2017 provided growers an additional option for broadleaf weed control. Because non-DR soybean is sensitive to low concentrations of dicamba, postemergence dicamba applications present growers choosing to plant non-DR soybean with concerns of damage from off-target movement through physical drift and volatilization of the herbicide as well as tank-contamination from prior dicamba application. Consequences of low-dose dicamba exposure on sensitive vegetative and reproductive soybean have been well researched, but little is known regarding the …

Association Mapping For Soybean (Glycine Max L. Merr.) Protein And Oil Content, Joseph Najjar

Graduate Theses and Dissertations

Soybean [Glycine Max (L.) Merrill] is an important economic crop because of its high content of seed protein and oil. Seed oil and protein content are quantitative inherited traits. The genetics of seed protein and oil levels have been extensively studied, with 367 QTL reported for protein and 475 QTL reported for oil to date. Validation of such QTLs, and identification of easily-automatable molecular markers around these QTL will aid the progression of breeding for such traits. The focus of this research was to discover novel and verify previously reported QTL related to protein and/or oil content via Genome-Wide Association …

Sensitivity Of Enlisttm And Roundup Ready Xtend® Technologies To Auxin Herbicides And Comparison Of Resistance To Susceptible Cotton And Soybean Cultivars, James Rose

Graduate Theses and Dissertations

Increases in weed resistance have led to the development of new herbicide-resistant crop technologies, namely the Roundup Ready Xtend® and EnlistTM weed control systems. These new technologies provide resistance to applications of the synthetic auxins dicamba or 2,4-D, respectively, in traited cotton or soybean. It is unknown if there is any resistance to other herbicides within the synthetic auxin group (WSSA Group 4) in these traited crops. Experiments were conducted with auxin herbicides representing all five families within WSSA Group 4. Herbicides were applied either preemergence (PRE) or postemergence (POST) relative to the crop in a single application. Applications were …

Genetics Of Physiological Traits Associated With Drought Tolerance In Soybean (Glycine Max), Sumandeep Kaur Bazzer

Graduate Theses and Dissertations

Soybean (Glycine max L.) is one of the major row crops in the United States, and its production is often limited by drought stress. Physiological traits from exotic germplasm that confer drought tolerance may be useful in improving commercial soybean production. For example, carbon isotope ratio (δ13C) is positively correlated with water use efficiency (WUE), and nitrogen isotope ratio (δ15N) is negatively correlated with N2 fixation; canopy temperature (CT) is an indicator for genetic variation in transpiration and stomatal conductance. Therefore, the objectives of this research were to identify the genomic regions associated with: (1) δ13C and δ15N using a …

Developing A High-Throughput Method To Screen Soybean Germplasm For Hypoxia Tolerance In Hydroponic Systems, Derrick Harrison

Graduate Theses and Dissertations

Soybean [Glycine max (Merr.)], the second most planted crop in the United States, is sensitive to field flooding due to depletion of oxygen and accumulation of CO2 in the rhizosphere. There is a need to breed cultivars that are adapted to areas prone to flooding, but field evaluations in the U.S. are limited because of time (one generation per year) and availability of resources (impermeable soils, irrigation, equipment to build levees). The purpose of this study was to develop and execute a protocol of germplasm screening for hypoxia tolerance using hydroponics in a controlled greenhouse environment. Germination rates and vigor …

Cover Cropping In Soybean-Corn Rotation System: Economic, Agronomic, And Soil Fertility Impact, Jose Rodolfo Mite

LSU Doctoral Dissertations

Planting winter-annual cover crops prevent soil erosion, reduces water runoff, and improves soil structure and soil quality. This research was conducted from 2017 to 2019 to evaluate the nutrient turnover of different species of cover crops in soils under different row crop production systems in Northeast and Central Louisiana. In Northeast Louisiana (Site 1, 2, and 3), treatments (cover crops and no cover crop) were arranged in a strip trial with three replications. At the Ben Hur Research Station, the treatments included three planting dates (September, October, and November) with [7 kg ha^-1 of phosphorus (P) and potassium (K)] …

Soybean Root Exudates Increase The Physiological Diversity Of Bacteria In Cadmium-Treated Soil, Leily Kazemi Movahed

Electronic Thesis and Dissertation Repository

Three soybean cultivars with contrasting retention of cadmium (Cd) in the root were grown in Cd-spiked nutrient solution and used to determine that symplastic compartmentalization of Cd in roots is probably responsible for retention of Cd in roots. Roots of the low Cd-accumulator AC Hime treated with 30 mM Cd exuded up to 10-fold higher concentrations of citric, succinic, fumaric and malic acids into the hydroponic solution when compared to control; concentrations of the same organic acids from the high Cd-accumulator Westag 97 increased by up to 3-fold. The same cultivars were grown in Cd-spiked soil and the physiological profiles …

In-Season Crop Management Effect On Soybean Yield And Grain Quality In Eastern South Dakota, Kelsey Bergman

Electronic Theses and Dissertations

Soybean [Glycine max (L.) Merr.] yield has increased over time by introduction of newer varieties and improved agronomic practices. However, grain protein concentration has been decreasing at the same time. New field studies in 2018 and 2019 investigated inseason crop management practices effect such as use of foliar protection application, fertilizer application, different maturity length or different planting dates on grain yield and grain quality. The studies were established near Brookings, SD and Beresford, SD. In-season treatments were targeting nutrient availability or protecting soybean canopy during the grain filling period. Such treatments included the use of fungicide, insecticide, or supplying …

Characterization Of Bacterial Endophytes Isolated From Brassica Carinata And Their Potential Use To Decrease Nutrient Requirements In Crops, Alex Soupir

Electronic Theses and Dissertations

Bacterial endophytes have the capability to enhance plant growth by producing plant growth hormones, solubilizing phosphates, suppressing pathogenic fungi, and reducing plant stress hormones. These capabilities make them desirable limiting the amount of nutrients and pesticides that are applied to crops. Through these assays and isolations, it is possible to identify novel bacterial species. In-vitro testing had shown 9 of the 20 isolates possess the ability to produce indole-3-acetic acid (IAA) with Pantoea agglomerans BC09 producing a concentration of 30.2 ng/μl over 4 days. BC09, Bacillus subilis BC10, and Pantoea sp. BC12 were able to solubilize calcium phosphate, 7 endophytes …

The Effects Of Seed-Applied Fluopyram On Root Penetration And Development Of Meloidogyne Incognita On Cotton And Soybean, Tracy Hawk

Graduate Theses and Dissertations

Plant-parasitic nematodes are major pests of cotton and soybean in Arkansas, and across the Southern United States. These nematodes cause more than $3 billion worth of crop losses each year, in part due to lack of available control tactics, such as nematicides. Fluopyram has recently been registered as a seed-treatment nematicide in agronomic crops. The toxicity of fluopyram against Meloidogyne incognita infection has been reported, however, information on root protection provided by fluopyram against Meloidogyne incognita is lacking. The first objective of this research was to evaluate the effect seed-applied fluopyram had on nematode development, root galling, and reproduction on …