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Genetic Characterization Of Centromere-Mediated Uniparental Genome Elimination In Arabidopsis, Diana Spencer
Genetic Characterization Of Centromere-Mediated Uniparental Genome Elimination In Arabidopsis, Diana Spencer
Electronic Theses and Dissertations
The production of haploids through crossing was first discovered from instances of interspecies crosses in Nicotiana spp. in 1924. Since then, haploid induction crosses has been used to improve plant breeding programs and commercially utilized in a number of crop industries. The generation of doubled haploid instantaneously creates a pure homozygous line, therefore eliminating the need for several generations of inbreeding. There are several pathways to induce haploids in plants: of these methods, centromere-mediated genome elimination pathway engenders the highest haploid induction rate (HIR) with up to 45% in Arabidopsis compared to 15% through phospholipase-mediated haploid induction in maize. Centromere-mediated …
Dynamics Of Redox-Driven Molecular Processes In Local And Systemic Plant Immunity, Philip Berg
Dynamics Of Redox-Driven Molecular Processes In Local And Systemic Plant Immunity, Philip Berg
Theses and Dissertations
The work here presents two main parts. In the first part, chapters 1 – 3 focus on dynamical systems modeling in plant immunity, whereas chapters 4 – 6 describe contributions to computational modeling and analysis of proteomics and genomics data. Chapter 1 investigates dynamical and biochemical patterns of reversibly oxidized cysteines (RevOxCys) during effector-triggered immunity (ETI) in Arabidopsis, examines the regulatory patterns associated with Arabidopsis thimet oligopeptidase 1 and 2’s (TOP1 and TOP2), roles in the RevOxCys events during ETI, and analyzes the redox phenotype of the top1top2 mutant. The second chapter investigates the peptidome dynamics during ETI …
Determination Of The Functional Role Of Rab-Ggt In Physcomitrium Patens., Hyun Jin Jung
Determination Of The Functional Role Of Rab-Ggt In Physcomitrium Patens., Hyun Jin Jung
Electronic Theses and Dissertations
Protein prenylation, a common lipid post-translational modification, is required for growth and development in eukaryotes. Rab geranylgeranylation involves the addition of one or two 20-carbon geranylgeranyl moieties to Rab-GTPase target proteins, which regulate intracellular vesicle trafficking. The reaction is carried out by heterodimeric Rab geranylgeranyltransferase (Rab-GGT), which is composed of two associated α- and β-subunits, with the assistance of an additional protein called Rab escort protein (REP). Loss of function of the Rab-GGT α subunit RGTA1 has not been reported in any plant. While knockout of either of the two β subunits RGTB1 or RGTB2 results in …