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Applications Of Site-Specific Recombination Systems In Transgene Expression And Marker Gene Removal, Mehmet Aydin Akbudak
Applications Of Site-Specific Recombination Systems In Transgene Expression And Marker Gene Removal, Mehmet Aydin Akbudak
Graduate Theses and Dissertations
Site Specific Recombination systems, such as FLP-FRT and Cre-lox, have been successfully used for site-specific gene integration and marker-gene deletion in plant systems. They are very useful tools in the integration of single-copy full-length transgene cassettes into the genome because the transgene integration via conventional methods often generate multi-copy locus. Such complex locus containing direct and inverted repeats of full-length and truncated copies of the transgene cassette generate aberrant RNA resulting in gene silencing. Therefore, for stable gene expression, a single copy transgene locus is preferred. However, even single copy locus sometimes succumbs to gene silencing. Although the mechanism is …
Investigating Heavy Metal Accumulation And Oxidative Stress Tolerance Of Non-Accumulators And Hyperaccumulators In The Brassicaceae Plant Family, Fritzie Joy Into
Investigating Heavy Metal Accumulation And Oxidative Stress Tolerance Of Non-Accumulators And Hyperaccumulators In The Brassicaceae Plant Family, Fritzie Joy Into
Theses and Dissertations - UTB/UTPA
Physiological and enzymatic analysis indicated that the non-accumulator A. thaliana showed an oxidative stress response in all assays except for protein carbonylation. Other non-accumulator plant species showed a significant difference in oxidative stress response in the TBARS assay; however, for some assays no stress response was evident. The hyperaccumulator plant species showed no significant difference in oxidative stress as indicated by the all of the assays with the exception of T. montanum var. montanum which showed an oxidative stress response in the SOD assay. Basal catalase enzyme activity was notably higher in the hyperaccumulators T. montanum var. montanum and T. …
Analysis Of The Response Of Medicago Truncatula Calcium Oxalate Mutants To Abiotic Stress, Wayra Gabriela Navia-Gine
Analysis Of The Response Of Medicago Truncatula Calcium Oxalate Mutants To Abiotic Stress, Wayra Gabriela Navia-Gine
Graduate Theses and Dissertations
Calcium oxalate crystals are found in most plant species. In Medicago truncatula wild-type A17, crystals accumulate in leaves along the secondary veins and the only role attributed to them so far is defense against chewing insects. Calcium oxalate deficient (cod) mutants were isolated in M. truncatula; the cod mutants include cod5, which completely lack crystals in the leaves and cod6 that accumulates fewer and smaller crystals compared to A17. We analyzed gene expression in the cod mutants and A17 using GeneChip® Medicago Genome Arrays and found important differences in transcriptome between the three genotypes. In particular, we found a gene …
Ethnobotanical History And Seed Oil Chemistry Of The Physic Nut, Jatropha Curcas, Nathan M. Leclear
Ethnobotanical History And Seed Oil Chemistry Of The Physic Nut, Jatropha Curcas, Nathan M. Leclear
Theses and Dissertations - UTB/UTPA
Jatropha curcas L., Euphorbiaceae, is a small tree native to the neotropics. Despite renewed economic interests in Physic Nut, mainly for biodiesel production, a comprehensive analysis of the ethnobotanical history of J. curcas is lacking. An account of the pre-Columbian neotropical distribution of this species and its historical uses are presented. Using capillary gas chromatography, studies were conducted to quantify the natural variation in seed oil content and fatty acid composition of the seed oil from several species of Jatropha, including assessment of how these parameters change during seed maturation. Natural variation in seed oil content was detected (F7,93 = …