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Insights Into The World Of Pea Nodulation Using The Low Nodulator R50, Scott Clemow
Insights Into The World Of Pea Nodulation Using The Low Nodulator R50, Scott Clemow
Theses and Dissertations (Comprehensive)
Cytokinin oxidase (CKX) is the enzyme responsible for the degradation of cytokinin, a class of adenine-based plant hormones that stimulate cell division, among other physiological processes. The pea mutant R50 is characterized by having a pale leaf phenotype, dwarf stature, few lateral roots, low nodule formation, and elevated levels of endogenous cytokinins in its shoots, roots and nodules. When compared to that of the wild-type Sparkle, total CKX activity is low but the transcript levels of PsCKX1 are significantly higher. In this study, I investigated the expression of PsCKX1 throughout the development of a nodule and the localization of PsCKX1 …
The Pleiotropic Mutant R50 (Sym16): A Link Between Cytokinin And Starch Metabolism May Explain Its Seed Phenotype, Chengli Long
The Pleiotropic Mutant R50 (Sym16): A Link Between Cytokinin And Starch Metabolism May Explain Its Seed Phenotype, Chengli Long
Theses and Dissertations (Comprehensive)
Cytokinins are a class of adenine-based plant hormones that stimulate cell divisions and are therefore essential for organ growth and development. The role that cytokinin plays in nodulation and that of R50 (syml6) in particular is very complex. In this study, a cytokinin antagonist and a cytokinin oxidase (CKX) inhibitor were used as tools to study the effects of cytokinin perception or cytokinin degradation on nodulation in pea, the cytokinin transduction pathway, especially that induced by CRE1/AHK4, and CKX play a significant role in pea nodulation. Comparison of the full length sequences PsCKX2 cDNAs from Sparkle and R50 …
Molecular Studies Of 5-Azacytidine-Induced Early-Flowering Lines Of Flax, Megan A. House
Molecular Studies Of 5-Azacytidine-Induced Early-Flowering Lines Of Flax, Megan A. House
Theses and Dissertations (Comprehensive)
Several early-flowering flax (Linum usitatissimum L.) lines were derived from treatment of germinating seeds with 5-azacytidine in 1990. These lines are also shorter, have fewer leaves, and their DNA is hypomethylated, relative to their corresponding controls. The work presented in this thesis used early-flowering and control lines of the Royal (R) flax genotype, and the Large (L) flax genotroph. Firstly, levels of cytosine methylation were measured over a 24-hour period in the early-flowering line RE2 and its control (RC), using an HPLC method. Secondly, to determine the response of the flax lines to short-day conditions, control and early-flowering …