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Low Co-Cultivation Temperature At 20oc Improved Agrobacterium Tumefaciens-Mediated Transformation Of Tobacco Leaf Disks, Guiying Su
LSU Master's Theses
I investigated the effect of co-cultivation temperature and binary Ti vectors on the fresh weight yield of leaf disks of tobacco (Nicotiana tabacum L. cv. Xanthi (nn, Smith) after Agrobacterium tumefaciens-mediated transformation. I concluded that the temperature at 20ºC during co-cultivation of leaf disks with A. tumefaciens was the most critical factor on the reproducible enhancement of fresh weight yield. The highest fresh weight increase and presumably transformation efficiency was achieved under the following experimental conditions: A. tumefaciens strain LBA4404 containing pLSU binary Ti vector was grown overnight at 28oC in liquid Agrobacterium medium and the concentration was adjusted to …
Physiological, Biochemical And Molecular Characteristics Associated With Virulence Of Burkholderia Glumae: The Major Causative Agent Of Bacterial Panicle Blight Of Rice, Hari Sharan Karki
Physiological, Biochemical And Molecular Characteristics Associated With Virulence Of Burkholderia Glumae: The Major Causative Agent Of Bacterial Panicle Blight Of Rice, Hari Sharan Karki
LSU Master's Theses
Burkholderia glumae is the bacterial pathogen causing bacterial panicle blight disease of rice (BPB). The pathogen, whose growth and pathogenicity is favored by high temperatures, has become a serious threat to rice production around the world possibly due to the current global climate changes. In this study, previously reported avirulent B. glumae strains were characterized in respect to the production of known virulence factors, toxoflavin, lipase and motility, and potential virulence factors, including polygalacturnase and type III secretion system (hypersensitive response). Considerable phenotypic variation was observed among the strains tested. Virulence of the B. glumae strains was closely related to …
A Comparison Of Soil And Corn Kernel Aspergillus Flavus Populations: Evidence For Niche Specialization, Rebecca Ruth Sweany
A Comparison Of Soil And Corn Kernel Aspergillus Flavus Populations: Evidence For Niche Specialization, Rebecca Ruth Sweany
LSU Master's Theses
Aspergillus flavus is an opportunistic fungal pathogen that infects peanuts, cotton, corn and tree nuts. Aspergillus flavus is a major problem globally due to the production of acutely toxic and carcinogenic aflatoxins. Louisiana climatic conditions lead to annual threats of corn aflatoxin contamination. The purpose of this study was to determine the specific ability of different strains of A. flavus to infect corn. Five soil samples and 10 corn ears were collected from each of seven corn fields throughout Louisiana. In addition, Francis Deville of Monsanto Company collected 7, 6, 2, and 4 soil samples and corn ears from four …
Effects Of Environmental Variables And Crop Growth On Development Of Brown Rust Epidemics In Sugarcane, Wilmer Anibal Barrera
Effects Of Environmental Variables And Crop Growth On Development Of Brown Rust Epidemics In Sugarcane, Wilmer Anibal Barrera
LSU Master's Theses
Natural epidemics of brown rust, caused by Puccinia melanocephala, affecting sugarcane were studied to determine the crop and/or environmental factors that affect epidemic onset, severity and eventual decline. Environmental and crop growth variables were monitored along with disease severity in two susceptible cultivars, LCP 85-384 and Ho 95-988, each grown at a different location in Louisiana. During two seasons, correlation and multiple regression analyses identified leaf wetness and temperature as important determinants of disease severity for both cultivars. The results suggested that crop growth variables were not determinants for epidemics. Controlled conditions experiments assessing the interaction of leaf wetness and …