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Study Of Host-Fungus Interactions Between Soybean And Phakopsora Pachyrhizi Using Proteomics, Sunjung Park
Study Of Host-Fungus Interactions Between Soybean And Phakopsora Pachyrhizi Using Proteomics, Sunjung Park
LSU Doctoral Dissertations
Asian soybean rust, caused by Phakopsora pachyrhizi, is an emerging disease in the continental U. S. and resistant commercial varieties have not been reported. In an effort to understand the interactions during rust infection of soybean, protein profile changes were examined over a 14-day period in soybean leaves of one susceptible commercial line (Pioneer 93M60) with or without soybean rust inoculation using proteomics in this study. Forty protein spots differentially expressed after rust inoculation were identified and fourteen of them were recovered and sequenced. These included proteins involved in plant defense, stress, metabolism, and other biological processes. During the time-course …
New Binary Ti Vectors With Co-Directional Replicons For Agrobacterium Tumefaciens-Mediated Transformation Of Higher Plants, Seokhyun Lee
New Binary Ti Vectors With Co-Directional Replicons For Agrobacterium Tumefaciens-Mediated Transformation Of Higher Plants, Seokhyun Lee
LSU Doctoral Dissertations
Small, high-yielding binary Ti vectors of Agrobacterium tumefaciens were constructed. All four basic components of the vector, ColE1 replicon (715 bp) for Escherichia coli, VS1 replicon (2,659 bp) for A. tumefaciens, a bacterial kanamycin resistance gene (999 bp), and the T-DNA region (170 bp), were modified to reduce the vector size to 4,566 bp and to introduce a number of mutations to increase the copy number and other functionality. The transcriptional direction of VS1 replicon can be the same as that of ColE1 replicon (co-directional transcription), or opposite (head-on transcription) as in the case of widely used vectors (pPZP or …