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Low Co-Cultivation Temperature At 20oc Improved Agrobacterium Tumefaciens-Mediated Transformation Of Tobacco Leaf Disks, Guiying Su
LSU Master's Theses
I investigated the effect of co-cultivation temperature and binary Ti vectors on the fresh weight yield of leaf disks of tobacco (Nicotiana tabacum L. cv. Xanthi (nn, Smith) after Agrobacterium tumefaciens-mediated transformation. I concluded that the temperature at 20ºC during co-cultivation of leaf disks with A. tumefaciens was the most critical factor on the reproducible enhancement of fresh weight yield. The highest fresh weight increase and presumably transformation efficiency was achieved under the following experimental conditions: A. tumefaciens strain LBA4404 containing pLSU binary Ti vector was grown overnight at 28oC in liquid Agrobacterium medium and the concentration was adjusted to …